Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070293051> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2070293051 abstract "Introduction: In recent years, accumulating experimental evidence has suggested that tumors have a hierarchical organisation where only a subset of parenchymal cells, the cancer stem cells (CSCs), has tumor‐initiating properties. To date, several antibodies directed against surface antigens have been employed to prospectively isolate CSCs from a broad spectrum of malignancies, though not from esophageal adenocarcinoma. Here, we conducted a series of experiments to test whether Barrett9s esophagus and esophageal adenocarcinoma may serve as a disease model for the cancer stem cell concept. Methods: We employed a panel of previously established CSC markers (CD24, CD29, CD44, CD133, CD166, EpCAM) for immunohistochemistry (IHC) analysis and in vivo transplantation assays. Transplantation of serially diluted bulk cancer cells of human tumors into NOD‐SCID mice was performed to investigate the presence of CSCs in esophageal adenocarcinoma. Analogous assays were carried out by injecting esophageal cancer cells sorted by FACS using the same panel of CSC markers as employed for IHC analysis. Results: IHC revealed that the intensity of CD24 and CD29 staining tended to increase along the metaplasia — dysplasia — carcinoma sequence. Cells positive for CD24 or CD29 were mainly located in the basal compartment of the metaplastic and dysplastic epithelium. CD44 showed membranous staining mainly in adenocarcinomas, whereas CD166 revealed pronounced cytoplasmic staining in all stages. Serial transplantation experiments with bulk tumor cells indicated that the frequency of tumor‐initiating cells in the Lin‐ population of esophageal adenocarcinoma is approximately 1:50,000 cells. However, to date no tumor growth was observed when tumor cells of subpopulations expressing the above‐described CSC markers were transplanted at lower multiplicities. Conclusions: Tumor‐initiating cells in esophageal adenocarcinoma are rare, reflecting a hierarchal organisation. However, surface‐antigens that have been established as CSC markers in other malignancies do not seem to enrich for tumor initiating cells in esophageal adenocarcinoma. Citation Information: Cancer Res 2009;69(23 Suppl):C6." @default.
- W2070293051 created "2016-06-24" @default.
- W2070293051 creator A5005487497 @default.
- W2070293051 creator A5006983831 @default.
- W2070293051 creator A5043992572 @default.
- W2070293051 creator A5051658337 @default.
- W2070293051 creator A5052116403 @default.
- W2070293051 creator A5058967460 @default.
- W2070293051 creator A5063731084 @default.
- W2070293051 creator A5072902637 @default.
- W2070293051 date "2009-12-01" @default.
- W2070293051 modified "2023-09-26" @default.
- W2070293051 title "Abstract C6: Barrett's esophagus and esophageal adenocarcinoma: A paradigm for the cancer stem cell model?" @default.
- W2070293051 doi "https://doi.org/10.1158/0008-5472.fbcr09-c6" @default.
- W2070293051 hasPublicationYear "2009" @default.
- W2070293051 type Work @default.
- W2070293051 sameAs 2070293051 @default.
- W2070293051 citedByCount "0" @default.
- W2070293051 crossrefType "proceedings-article" @default.
- W2070293051 hasAuthorship W2070293051A5005487497 @default.
- W2070293051 hasAuthorship W2070293051A5006983831 @default.
- W2070293051 hasAuthorship W2070293051A5043992572 @default.
- W2070293051 hasAuthorship W2070293051A5051658337 @default.
- W2070293051 hasAuthorship W2070293051A5052116403 @default.
- W2070293051 hasAuthorship W2070293051A5058967460 @default.
- W2070293051 hasAuthorship W2070293051A5063731084 @default.
- W2070293051 hasAuthorship W2070293051A5072902637 @default.
- W2070293051 hasConcept C121608353 @default.
- W2070293051 hasConcept C126322002 @default.
- W2070293051 hasConcept C142724271 @default.
- W2070293051 hasConcept C1491633281 @default.
- W2070293051 hasConcept C2775894508 @default.
- W2070293051 hasConcept C2777819096 @default.
- W2070293051 hasConcept C2778589982 @default.
- W2070293051 hasConcept C2779742542 @default.
- W2070293051 hasConcept C2780932548 @default.
- W2070293051 hasConcept C2781182431 @default.
- W2070293051 hasConcept C28328180 @default.
- W2070293051 hasConcept C2908647359 @default.
- W2070293051 hasConcept C2911091166 @default.
- W2070293051 hasConcept C502942594 @default.
- W2070293051 hasConcept C54355233 @default.
- W2070293051 hasConcept C55427017 @default.
- W2070293051 hasConcept C71924100 @default.
- W2070293051 hasConcept C86803240 @default.
- W2070293051 hasConcept C99454951 @default.
- W2070293051 hasConceptScore W2070293051C121608353 @default.
- W2070293051 hasConceptScore W2070293051C126322002 @default.
- W2070293051 hasConceptScore W2070293051C142724271 @default.
- W2070293051 hasConceptScore W2070293051C1491633281 @default.
- W2070293051 hasConceptScore W2070293051C2775894508 @default.
- W2070293051 hasConceptScore W2070293051C2777819096 @default.
- W2070293051 hasConceptScore W2070293051C2778589982 @default.
- W2070293051 hasConceptScore W2070293051C2779742542 @default.
- W2070293051 hasConceptScore W2070293051C2780932548 @default.
- W2070293051 hasConceptScore W2070293051C2781182431 @default.
- W2070293051 hasConceptScore W2070293051C28328180 @default.
- W2070293051 hasConceptScore W2070293051C2908647359 @default.
- W2070293051 hasConceptScore W2070293051C2911091166 @default.
- W2070293051 hasConceptScore W2070293051C502942594 @default.
- W2070293051 hasConceptScore W2070293051C54355233 @default.
- W2070293051 hasConceptScore W2070293051C55427017 @default.
- W2070293051 hasConceptScore W2070293051C71924100 @default.
- W2070293051 hasConceptScore W2070293051C86803240 @default.
- W2070293051 hasConceptScore W2070293051C99454951 @default.
- W2070293051 hasLocation W20702930511 @default.
- W2070293051 hasOpenAccess W2070293051 @default.
- W2070293051 hasPrimaryLocation W20702930511 @default.
- W2070293051 hasRelatedWork W1525061042 @default.
- W2070293051 hasRelatedWork W1562191471 @default.
- W2070293051 hasRelatedWork W1706553974 @default.
- W2070293051 hasRelatedWork W2011561516 @default.
- W2070293051 hasRelatedWork W2023445598 @default.
- W2070293051 hasRelatedWork W2062238622 @default.
- W2070293051 hasRelatedWork W2064909516 @default.
- W2070293051 hasRelatedWork W2098435943 @default.
- W2070293051 hasRelatedWork W2135635131 @default.
- W2070293051 hasRelatedWork W2147732443 @default.
- W2070293051 hasRelatedWork W2192486833 @default.
- W2070293051 hasRelatedWork W2253401643 @default.
- W2070293051 hasRelatedWork W2313760878 @default.
- W2070293051 hasRelatedWork W2398891622 @default.
- W2070293051 hasRelatedWork W2494977919 @default.
- W2070293051 hasRelatedWork W2535562092 @default.
- W2070293051 hasRelatedWork W2592759488 @default.
- W2070293051 hasRelatedWork W2904319062 @default.
- W2070293051 hasRelatedWork W2975665350 @default.
- W2070293051 hasRelatedWork W3199540023 @default.
- W2070293051 isParatext "false" @default.
- W2070293051 isRetracted "false" @default.
- W2070293051 magId "2070293051" @default.
- W2070293051 workType "article" @default.