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- W2070295619 abstract "İdrarda testosteron (T) ve epitestosteronun (E) tayini için, ince tabaka kromatografisi ile kombine basit ve duyarlı gaz kromatografik bir yöntem tanımlanmıştır. İdrardaki testosteron ve epitestosteron asit hidrolizi ve ekstraksiyondan sonra ince tabaka kromatografisi (İTK) ile ayrılmış ve elüe edilmiştir. Elüat asetillenmiş, tekrar İTK ile izole edilmiş ve iç standart ilave edilip gaz sıvı kromatografisinde (GSK) analiz edilmiştir. Bu yöntemle verim testosteron için % 73.79 ± 3.9 ve epitestosteron için % 75.94 ± 7.9, duyarlılık ise 0.05 µg olarak bulunmuştur. Geliştirilen yöntem normal erkek ve kadın idrarları ile ilaç verilen kişilerin idrarlarına uygulanarak T E oranlan saptanmıştır. Normal erkek idrarlarında bu oran 1.96 ± 0.62, normal kadın idrarlarında 2.62 ± 0.32, ilaç verilen kişilerin idrarlarında ise 4.88 ± 1.78 olarak bulunmuştur. Bulgular doping kontrolü açısından değerlendirilmiştir. and then eluted. The eluates acetylated, applied to TLC for further isolation and analyzed by gas liquid chromatography (GLC) by using internal standard. The recovery was found 73.79 ± 3.9 % for testosterone and 75.94 + 7.9 % for epitestosterone with 0.05 µg sensitivity. T/E ratio in urine samples of normal males, females and testosterone administred male subjects were determined by the described" @default.
- W2070295619 created "2016-06-24" @default.
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- W2070295619 date "1989-01-01" @default.
- W2070295619 modified "2023-10-17" @default.
- W2070295619 title "İdrarda Testosteron ve Epitestosteronun İnce Tabaka Kromatografisi ve Gaz Sıvı Kromatografisi İle Tayini" @default.
- W2070295619 doi "https://doi.org/10.1501/eczfak_0000000304" @default.
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