FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070296004> ?p ?o ?g. }

• W2070296004 endingPage "1228" @default.
• W2070296004 startingPage "1220" @default.
• W2070296004 abstract "Transforming growth factor-beta (TGFbeta) plays a central role in the stimulation of matrix production during liver fibrosis. The action of TGFbeta in different systems has been shown to be influenced by alpha2-macroglobulin (alpha2M), a serum protein with strong protease-scavenging and cytokine-binding properties.In the present study, alpha2M derived from normal human plasma has been tested for its ability to modulate the TGFbeta-induced collagen production by human liver fat-storing cells (FSC), which had transformed into alpha-smooth muscle actin-expressing myofibroblasts in culture.Alpha2M has been tested after activation with methylamine (alpha2M-Me), an in vitro equivalent of protease activated alpha2M. The binding of 125I-TGFbeta1 to activated forms of alpha2M was demonstrated by rate electrophoresis. Collagen synthesis was examined in human liver myofibroblast cultures obtained from three different human livers by incorporation of 3H-proline into TCA-precipitable, specific collagenase degradable proteins. Uptake of alpha2M was studied by means of immunofluorescence.TGFbeta (1 ng/ml) significantly stimulated collagen synthesis of controls in the absence of TGFbeta. Alpha2M-Me reduced this TGFbeta-induced collagen synthesis dose-dependently, reaching significant inhibition from 10 microg/ml alpha2M-Me onward. Upon addition of 100 microg/ml alpha2M-Me the effect of TGFbeta was reduced by 60% to 128+/-31% (mean+/-SD) of control values in the absence of TGFbeta. Human liver myofibroblasts endocytosed alpha2M-Me added to the cultures as detected by immunofluorescence. Accordingly, reduction of TGFbeta-activity by alpha2M-Me may be explained by receptor-mediated clearance of alpha2M-TGFbeta complexes by the cells.TGFbeta-induced collagen formation by human liver myofibroblasts obtained from three different livers is reduced in vitro by activated alpha2M. From these results, we hypothesize that alpha2M may have an antifibrogenic effect in vivo by interference with TGFbeta-induced matrix synthesis during liver fibrosis." @default.
• W2070296004 created "2016-06-24" @default.
• W2070296004 creator A5000062119 @default.
• W2070296004 creator A5039243503 @default.
• W2070296004 creator A5039491429 @default.
• W2070296004 creator A5055592755 @default.
• W2070296004 creator A5084417339 @default.
• W2070296004 date "1997-07-01" @default.
• W2070296004 modified "2023-09-25" @default.
• W2070296004 title "Transforming growth factor-β-induced collagen synthesis by human liver myofibroblasts is inhibited by α2-macroglobulin" @default.
• W2070296004 cites W1494827648 @default.
• W2070296004 cites W1512861794 @default.
• W2070296004 cites W1551935161 @default.
• W2070296004 cites W1556540426 @default.
• W2070296004 cites W1560768456 @default.
• W2070296004 cites W1569439329 @default.
• W2070296004 cites W1576487122 @default.
• W2070296004 cites W1591835416 @default.
• W2070296004 cites W1630663138 @default.
• W2070296004 cites W1758096854 @default.
• W2070296004 cites W1870388044 @default.
• W2070296004 cites W1952851700 @default.
• W2070296004 cites W1970938829 @default.
• W2070296004 cites W1972181390 @default.
• W2070296004 cites W1988769263 @default.
• W2070296004 cites W2002257314 @default.
• W2070296004 cites W2010257968 @default.
• W2070296004 cites W2012852656 @default.
• W2070296004 cites W2023368861 @default.
• W2070296004 cites W2041665122 @default.
• W2070296004 cites W2044803173 @default.
• W2070296004 cites W2046824846 @default.
• W2070296004 cites W2054876489 @default.
• W2070296004 cites W2055796324 @default.
• W2070296004 cites W2061881061 @default.
• W2070296004 cites W2064286345 @default.
• W2070296004 cites W2065430193 @default.
• W2070296004 cites W2068037889 @default.
• W2070296004 cites W2068934482 @default.
• W2070296004 cites W2074644545 @default.
• W2070296004 cites W2077683521 @default.
• W2070296004 cites W2083158390 @default.
• W2070296004 cites W2114138818 @default.
• W2070296004 cites W2121033933 @default.
• W2070296004 cites W2122081319 @default.
• W2070296004 cites W2125748383 @default.
• W2070296004 cites W2131211068 @default.
• W2070296004 cites W2136002823 @default.
• W2070296004 cites W2151376215 @default.
• W2070296004 cites W2162984201 @default.
• W2070296004 cites W2283733718 @default.
• W2070296004 cites W2323637528 @default.
• W2070296004 cites W2335838053 @default.
• W2070296004 cites W243631893 @default.
• W2070296004 cites W2912986512 @default.
• W2070296004 cites W30147252 @default.
• W2070296004 doi "https://doi.org/10.1016/s0168-8278(97)80455-2" @default.
• W2070296004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9210607" @default.
• W2070296004 hasPublicationYear "1997" @default.
• W2070296004 type Work @default.
• W2070296004 sameAs 2070296004 @default.
• W2070296004 citedByCount "40" @default.
• W2070296004 countsByYear W20702960042013 @default.
• W2070296004 countsByYear W20702960042014 @default.
• W2070296004 countsByYear W20702960042016 @default.
• W2070296004 countsByYear W20702960042017 @default.
• W2070296004 countsByYear W20702960042018 @default.
• W2070296004 countsByYear W20702960042019 @default.
• W2070296004 countsByYear W20702960042020 @default.
• W2070296004 countsByYear W20702960042021 @default.
• W2070296004 countsByYear W20702960042022 @default.
• W2070296004 crossrefType "journal-article" @default.
• W2070296004 hasAuthorship W2070296004A5000062119 @default.
• W2070296004 hasAuthorship W2070296004A5039243503 @default.
• W2070296004 hasAuthorship W2070296004A5039491429 @default.
• W2070296004 hasAuthorship W2070296004A5055592755 @default.
• W2070296004 hasAuthorship W2070296004A5084417339 @default.
• W2070296004 hasBestOaLocation W20702960042 @default.
• W2070296004 hasConcept C118131993 @default.
• W2070296004 hasConcept C126322002 @default.
• W2070296004 hasConcept C134018914 @default.
• W2070296004 hasConcept C153911025 @default.
• W2070296004 hasConcept C181199279 @default.
• W2070296004 hasConcept C185592680 @default.
• W2070296004 hasConcept C2778394429 @default.
• W2070296004 hasConcept C2781054842 @default.
• W2070296004 hasConcept C55493867 @default.
• W2070296004 hasConcept C71924100 @default.
• W2070296004 hasConcept C86803240 @default.
• W2070296004 hasConceptScore W2070296004C118131993 @default.
• W2070296004 hasConceptScore W2070296004C126322002 @default.
• W2070296004 hasConceptScore W2070296004C134018914 @default.
• W2070296004 hasConceptScore W2070296004C153911025 @default.
• W2070296004 hasConceptScore W2070296004C181199279 @default.
• W2070296004 hasConceptScore W2070296004C185592680 @default.
• W2070296004 hasConceptScore W2070296004C2778394429 @default.
• W2070296004 hasConceptScore W2070296004C2781054842 @default.
• W2070296004 hasConceptScore W2070296004C55493867 @default.

• next