FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070403963> ?p ?o ?g. }

• W2070403963 abstract "Myoepithelial cells (MEC) are essential to the maintenance of normal breast function, and loss of normal MEC function is commonly associated with breast cancer. Most established invasive breast carcinomas develop through an in situ phase known as ductal carcinoma in situ (DCIS). We have identified up-regulation of β6 integrin on MEC in a subset of DCIS. Normal MEC exhibit potent tumour suppressor function, but it is not clear whether this is compromised in DCIS. The aim of the present study is to investigate the effect of β6 expression on myoepithelial tumour suppressor function.Immunohistochemical analysis of DCIS of different grades with and without an invasive breast cancer was carried out to determine the β6 status. For in vitro studies magnetic bead sorting was used to isolate a pure normal-like myoepithelial cell line from the immortalised 1089 cell line (N-1089 MEC). These were used to generate overexpressing myoepithelial cells (DCIS-modified MEC) by β6 retroviral transduction. The lines were characterised by immuno-fluorescence and flow cytometry, and the tumour suppressor function of both lines was compared with primary normal and DCIS MEC in coculture with breast cancer cell lines.Analysis of a series of primary DCIS tissues (n > 400) demonstrated induction of β6 in MEC in a subset of cases, predominantly high grade. Upregulation was almost universal in DCIS associated with invasive disease. Initial characterisation of the 1089 line revealed a mixed phenotype from which pure MEC were selected on the basis of β4 integrin. This population exhibited all characteristic myoepithelial markers. Coculture assays demonstrated that N-1089 MEC could produce the same tumour suppressor effect as primary MEC, leading to significant reduction in tumour invasion (P < 0.001) and proliferation (P < 0.001). N-1089 MEC transduced with αvβ6 (DCIS-1089 MEC) demonstrated enhanced binding and migration to the β6 ligand LAP, and activation of a transforming growth factor beta reporter, indicating that the αvβ6 was functional. Whilst primary DCIS MEC showed loss of suppressor function (P < 0.05), DCIS-1089 MEC exhibit altered behaviour with a more migratory phenotype then the normal counterpart, but at least some of the tumour-suppressor function was maintained.We have shown that MEC exhibit an altered phenotype in DCIS with de novo expression of αvβ6. We have generated normal-like cell lines that exhibit all the characteristics of primary MEC and recapitulate primary MEC tumour suppressor function. Primary DCIS MEC show loss of suppressor function whereas β6-overexpressing MEC (which resemble DCIS-like MEC) promote or suppress breast cancer cell invasion in a cell-type-specific manner. These findings suggest that changes in MEC during DCIS may influence disease progression, and these cell lines provide a powerful model to study further the mechanisms involved." @default.
• W2070403963 created "2016-06-24" @default.
• W2070403963 creator A5008605236 @default.
• W2070403963 creator A5017945507 @default.
• W2070403963 creator A5074278844 @default.
• W2070403963 creator A5074918071 @default.
• W2070403963 creator A5076743192 @default.
• W2070403963 creator A5078072484 @default.
• W2070403963 date "2008-05-01" @default.
• W2070403963 modified "2023-10-18" @default.
• W2070403963 title "De novo expression of αvβ6 integrin by myoepithelial cells in ductal carcinoma in situ may be an important marker of disease progression" @default.
• W2070403963 cites W1973268287 @default.
• W2070403963 cites W2009007206 @default.
• W2070403963 cites W2062544730 @default.
• W2070403963 doi "https://doi.org/10.1186/bcr1912" @default.
• W2070403963 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3300731" @default.
• W2070403963 hasPublicationYear "2008" @default.
• W2070403963 type Work @default.
• W2070403963 sameAs 2070403963 @default.
• W2070403963 citedByCount "2" @default.
• W2070403963 countsByYear W20704039632013 @default.
• W2070403963 countsByYear W20704039632017 @default.
• W2070403963 crossrefType "journal-article" @default.
• W2070403963 hasAuthorship W2070403963A5008605236 @default.
• W2070403963 hasAuthorship W2070403963A5017945507 @default.
• W2070403963 hasAuthorship W2070403963A5074278844 @default.
• W2070403963 hasAuthorship W2070403963A5074918071 @default.
• W2070403963 hasAuthorship W2070403963A5076743192 @default.
• W2070403963 hasAuthorship W2070403963A5078072484 @default.
• W2070403963 hasBestOaLocation W20704039631 @default.
• W2070403963 hasConcept C104317684 @default.
• W2070403963 hasConcept C121608353 @default.
• W2070403963 hasConcept C126322002 @default.
• W2070403963 hasConcept C140884156 @default.
• W2070403963 hasConcept C142724271 @default.
• W2070403963 hasConcept C143998085 @default.
• W2070403963 hasConcept C150194340 @default.
• W2070403963 hasConcept C178790620 @default.
• W2070403963 hasConcept C185592680 @default.
• W2070403963 hasConcept C204232928 @default.
• W2070403963 hasConcept C2777546739 @default.
• W2070403963 hasConcept C2777661416 @default.
• W2070403963 hasConcept C2777822432 @default.
• W2070403963 hasConcept C2780140570 @default.
• W2070403963 hasConcept C2780862961 @default.
• W2070403963 hasConcept C502942594 @default.
• W2070403963 hasConcept C530470458 @default.
• W2070403963 hasConcept C54355233 @default.
• W2070403963 hasConcept C71924100 @default.
• W2070403963 hasConcept C81439078 @default.
• W2070403963 hasConcept C86803240 @default.
• W2070403963 hasConceptScore W2070403963C104317684 @default.
• W2070403963 hasConceptScore W2070403963C121608353 @default.
• W2070403963 hasConceptScore W2070403963C126322002 @default.
• W2070403963 hasConceptScore W2070403963C140884156 @default.
• W2070403963 hasConceptScore W2070403963C142724271 @default.
• W2070403963 hasConceptScore W2070403963C143998085 @default.
• W2070403963 hasConceptScore W2070403963C150194340 @default.
• W2070403963 hasConceptScore W2070403963C178790620 @default.
• W2070403963 hasConceptScore W2070403963C185592680 @default.
• W2070403963 hasConceptScore W2070403963C204232928 @default.
• W2070403963 hasConceptScore W2070403963C2777546739 @default.
• W2070403963 hasConceptScore W2070403963C2777661416 @default.
• W2070403963 hasConceptScore W2070403963C2777822432 @default.
• W2070403963 hasConceptScore W2070403963C2780140570 @default.
• W2070403963 hasConceptScore W2070403963C2780862961 @default.
• W2070403963 hasConceptScore W2070403963C502942594 @default.
• W2070403963 hasConceptScore W2070403963C530470458 @default.
• W2070403963 hasConceptScore W2070403963C54355233 @default.
• W2070403963 hasConceptScore W2070403963C71924100 @default.
• W2070403963 hasConceptScore W2070403963C81439078 @default.
• W2070403963 hasConceptScore W2070403963C86803240 @default.
• W2070403963 hasIssue "S2" @default.
• W2070403963 hasLocation W20704039631 @default.
• W2070403963 hasLocation W20704039632 @default.
• W2070403963 hasLocation W20704039633 @default.
• W2070403963 hasOpenAccess W2070403963 @default.
• W2070403963 hasPrimaryLocation W20704039631 @default.
• W2070403963 hasRelatedWork W107025639 @default.
• W2070403963 hasRelatedWork W1946670552 @default.
• W2070403963 hasRelatedWork W2011400119 @default.
• W2070403963 hasRelatedWork W2024189977 @default.
• W2070403963 hasRelatedWork W2055053153 @default.
• W2070403963 hasRelatedWork W2098284063 @default.
• W2070403963 hasRelatedWork W2412169407 @default.
• W2070403963 hasRelatedWork W260221542 @default.
• W2070403963 hasRelatedWork W2975326754 @default.
• W2070403963 hasRelatedWork W3094057919 @default.
• W2070403963 hasVolume "10" @default.
• W2070403963 isParatext "false" @default.
• W2070403963 isRetracted "false" @default.
• W2070403963 magId "2070403963" @default.
• W2070403963 workType "article" @default.