FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070451951> ?p ?o ?g. }

• W2070451951 endingPage "4569" @default.
• W2070451951 startingPage "4559" @default.
• W2070451951 abstract "Preparation of high-quality proteins from cotton fiber tissues is difficult due to high endogenous levels of polysaccharides, polyphenols, and other interfering compounds. To establish a routine procedure for the application of proteomic analysis to cotton fiber tissues, a new protocol for protein extraction was developed by optimizing a phenol extraction method combined with methanol/ammonium acetate precipitation. The protein extraction for 2-DE was remarkably improved by the combination of chemically and physically modified processes including polyvinylpolypyrrolidone (PVPP) addition, acetone cleaning, and SDS replacement. The protocol gave a higher protein yield and vastly greater resolution and spot intensity. The efficiency of this protocol and its feasibility in fiber proteomic study were demonstrated by comparison of the cotton fiber proteomes at two growth stages. Furthermore, ten protein spots changed significantly were identified by MS/tandem MS and their potential relationships to fiber development were discussed. To the best of our knowledge, this is the first time that a protocol for protein extraction from cotton fiber tissues appears to give satisfactory and reproductive 2-D protein profiles. The protocol is expected to accelerate the process of the proteomic study of cotton fibers and also to be applicable to other recalcitrant plant tissues." @default.
• W2070451951 created "2016-06-24" @default.
• W2070451951 creator A5035431515 @default.
• W2070451951 creator A5037286379 @default.
• W2070451951 creator A5063293122 @default.
• W2070451951 date "2006-11-01" @default.
• W2070451951 modified "2023-10-16" @default.
• W2070451951 title "An efficient protein preparation for proteomic analysis of developing cotton fibers by 2-DE" @default.
• W2070451951 cites W1523949295 @default.
• W2070451951 cites W1606423922 @default.
• W2070451951 cites W1899526134 @default.
• W2070451951 cites W1969497924 @default.
• W2070451951 cites W1972433895 @default.
• W2070451951 cites W1973114737 @default.
• W2070451951 cites W1983002021 @default.
• W2070451951 cites W1988419377 @default.
• W2070451951 cites W1989775081 @default.
• W2070451951 cites W2002631115 @default.
• W2070451951 cites W2007164264 @default.
• W2070451951 cites W2007960184 @default.
• W2070451951 cites W2009333744 @default.
• W2070451951 cites W2021236905 @default.
• W2070451951 cites W2030244758 @default.
• W2070451951 cites W2031861479 @default.
• W2070451951 cites W2035369337 @default.
• W2070451951 cites W2046726975 @default.
• W2070451951 cites W2048333869 @default.
• W2070451951 cites W2048399999 @default.
• W2070451951 cites W2058674203 @default.
• W2070451951 cites W2065295207 @default.
• W2070451951 cites W2065356685 @default.
• W2070451951 cites W2077912973 @default.
• W2070451951 cites W2090787790 @default.
• W2070451951 cites W2099121315 @default.
• W2070451951 cites W2114623563 @default.
• W2070451951 cites W2118577066 @default.
• W2070451951 cites W2121180756 @default.
• W2070451951 cites W2132901471 @default.
• W2070451951 cites W2133991317 @default.
• W2070451951 cites W2139299708 @default.
• W2070451951 cites W2144913010 @default.
• W2070451951 cites W2150630769 @default.
• W2070451951 cites W2159149313 @default.
• W2070451951 cites W75890918 @default.
• W2070451951 doi "https://doi.org/10.1002/elps.200600111" @default.
• W2070451951 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17058302" @default.
• W2070451951 hasPublicationYear "2006" @default.
• W2070451951 type Work @default.
• W2070451951 sameAs 2070451951 @default.
• W2070451951 citedByCount "85" @default.
• W2070451951 countsByYear W20704519512012 @default.
• W2070451951 countsByYear W20704519512013 @default.
• W2070451951 countsByYear W20704519512014 @default.
• W2070451951 countsByYear W20704519512015 @default.
• W2070451951 countsByYear W20704519512016 @default.
• W2070451951 countsByYear W20704519512017 @default.
• W2070451951 countsByYear W20704519512018 @default.
• W2070451951 countsByYear W20704519512019 @default.
• W2070451951 countsByYear W20704519512020 @default.
• W2070451951 countsByYear W20704519512021 @default.
• W2070451951 countsByYear W20704519512022 @default.
• W2070451951 countsByYear W20704519512023 @default.
• W2070451951 crossrefType "journal-article" @default.
• W2070451951 hasAuthorship W2070451951A5035431515 @default.
• W2070451951 hasAuthorship W2070451951A5037286379 @default.
• W2070451951 hasAuthorship W2070451951A5063293122 @default.
• W2070451951 hasConcept C104317684 @default.
• W2070451951 hasConcept C104397665 @default.
• W2070451951 hasConcept C135650642 @default.
• W2070451951 hasConcept C162356407 @default.
• W2070451951 hasConcept C178790620 @default.
• W2070451951 hasConcept C185592680 @default.
• W2070451951 hasConcept C2778004101 @default.
• W2070451951 hasConcept C2781213060 @default.
• W2070451951 hasConcept C31827203 @default.
• W2070451951 hasConcept C43617362 @default.
• W2070451951 hasConcept C46111723 @default.
• W2070451951 hasConcept C4725764 @default.
• W2070451951 hasConcept C519885992 @default.
• W2070451951 hasConcept C55493867 @default.
• W2070451951 hasConcept C67705224 @default.
• W2070451951 hasConcept C70899900 @default.
• W2070451951 hasConcept C8985478 @default.
• W2070451951 hasConceptScore W2070451951C104317684 @default.
• W2070451951 hasConceptScore W2070451951C104397665 @default.
• W2070451951 hasConceptScore W2070451951C135650642 @default.
• W2070451951 hasConceptScore W2070451951C162356407 @default.
• W2070451951 hasConceptScore W2070451951C178790620 @default.
• W2070451951 hasConceptScore W2070451951C185592680 @default.
• W2070451951 hasConceptScore W2070451951C2778004101 @default.
• W2070451951 hasConceptScore W2070451951C2781213060 @default.
• W2070451951 hasConceptScore W2070451951C31827203 @default.
• W2070451951 hasConceptScore W2070451951C43617362 @default.
• W2070451951 hasConceptScore W2070451951C46111723 @default.
• W2070451951 hasConceptScore W2070451951C4725764 @default.
• W2070451951 hasConceptScore W2070451951C519885992 @default.
• W2070451951 hasConceptScore W2070451951C55493867 @default.
• W2070451951 hasConceptScore W2070451951C67705224 @default.

• next