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- W2070600324 endingPage "e44912" @default.
- W2070600324 startingPage "e44912" @default.
- W2070600324 abstract "Embryonic stem cell-derived hepatocyte precursor cells represent a promising model for clinical transplantations to diseased livers, as well as for establishment of in vitro systems for drug metabolism and toxicology investigations. This study aimed to establish an in vitro culture system for scalable generation of hepatic progenitor cells. We used stable transgenic clones of murine embryonic stem cells possessing a reporter/selection vector, in which the enhanced green fluorescent protein- and puromycin N-acetyltransferase-coding genes are driven by a common alpha-fetoprotein gene promoter. This allowed for “live” monitoring and puromycin selection of the desired differentiating cell type possessing the activated alpha-fetoprotein gene. A rotary culture system was established, sequentially yielding initially partially selected hepatocyte lineage-committed cells, and finally, a highly purified cell population maintained as a dynamic suspension spheroid culture, which progressively developed the hepatic gene expression phenotype. The latter was confirmed by quantitative RT-PCR analysis, which showed a progressive up-regulation of hepatic genes during spheroid culture, indicating development of a mixed hepatocyte precursor-/fetal hepatocyte-like cell population. Adherent spheroids gave rise to advanced differentiated hepatocyte-like cells expressing hepatic proteins such as albumin, alpha-1-antitrypsin, cytokeratin 18, E-cadherin, and liver-specific organic anion transporter 1, as demonstrated by fluorescent immunostaining. A fraction of adherent cells was capable of glycogen storage and of reversible up-take of indocyanine green, demonstrating their hepatocyte-like functionality. Moreover, after transplantation of spheroids into the mouse liver, the spheroid-derived cells integrated into recipient. These results demonstrate that large-scale hepatocyte precursor-/hepatocyte-like cultures can be established for use in clinical trials, as well as in in vitro screening assays." @default.
- W2070600324 created "2016-06-24" @default.
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- W2070600324 date "2012-09-24" @default.
- W2070600324 modified "2023-10-17" @default.
- W2070600324 title "Differentiation and Selection of Hepatocyte Precursors in Suspension Spheroid Culture of Transgenic Murine Embryonic Stem Cells" @default.
- W2070600324 cites W1496136136 @default.
- W2070600324 cites W1571853300 @default.
- W2070600324 cites W1580549116 @default.
- W2070600324 cites W1748043009 @default.
- W2070600324 cites W1844312938 @default.
- W2070600324 cites W1870688656 @default.
- W2070600324 cites W1879968869 @default.
- W2070600324 cites W1948275303 @default.
- W2070600324 cites W1966450565 @default.
- W2070600324 cites W1967598939 @default.
- W2070600324 cites W1968699130 @default.
- W2070600324 cites W1971497482 @default.
- W2070600324 cites W1972137353 @default.
- W2070600324 cites W1972139648 @default.
- W2070600324 cites W1976183817 @default.
- W2070600324 cites W1977088306 @default.
- W2070600324 cites W1983707878 @default.
- W2070600324 cites W1987286203 @default.
- W2070600324 cites W1988801780 @default.
- W2070600324 cites W1988849672 @default.
- W2070600324 cites W1989069505 @default.
- W2070600324 cites W1989245663 @default.
- W2070600324 cites W1993438857 @default.
- W2070600324 cites W1994963086 @default.
- W2070600324 cites W1996884864 @default.
- W2070600324 cites W1998223524 @default.
- W2070600324 cites W1999459866 @default.
- W2070600324 cites W2006009416 @default.
- W2070600324 cites W2009359841 @default.
- W2070600324 cites W2011028760 @default.
- W2070600324 cites W2012777643 @default.
- W2070600324 cites W2014527236 @default.
- W2070600324 cites W2015884084 @default.
- W2070600324 cites W2017906550 @default.
- W2070600324 cites W2018708343 @default.
- W2070600324 cites W2019049336 @default.
- W2070600324 cites W2021718747 @default.
- W2070600324 cites W2022792733 @default.
- W2070600324 cites W2023419501 @default.
- W2070600324 cites W2024324088 @default.
- W2070600324 cites W2032105048 @default.
- W2070600324 cites W2032325711 @default.
- W2070600324 cites W2033662140 @default.
- W2070600324 cites W2033672481 @default.
- W2070600324 cites W2035481959 @default.
- W2070600324 cites W2040253875 @default.
- W2070600324 cites W2043875880 @default.
- W2070600324 cites W2046011628 @default.
- W2070600324 cites W2047454701 @default.
- W2070600324 cites W2049778754 @default.
- W2070600324 cites W2052190295 @default.
- W2070600324 cites W2058276205 @default.
- W2070600324 cites W2059108509 @default.
- W2070600324 cites W2065122365 @default.
- W2070600324 cites W2066758609 @default.
- W2070600324 cites W2069176893 @default.
- W2070600324 cites W2074991881 @default.
- W2070600324 cites W2075464063 @default.
- W2070600324 cites W2077547257 @default.
- W2070600324 cites W2077796674 @default.
- W2070600324 cites W2080037764 @default.
- W2070600324 cites W2080991023 @default.
- W2070600324 cites W2087135943 @default.
- W2070600324 cites W2091623520 @default.
- W2070600324 cites W2092727179 @default.
- W2070600324 cites W2093391705 @default.
- W2070600324 cites W2094689158 @default.
- W2070600324 cites W2100491180 @default.
- W2070600324 cites W2100715279 @default.
- W2070600324 cites W2105649511 @default.
- W2070600324 cites W2107277218 @default.
- W2070600324 cites W2114683402 @default.
- W2070600324 cites W2115562189 @default.
- W2070600324 cites W2116140480 @default.
- W2070600324 cites W2117597304 @default.
- W2070600324 cites W2127320439 @default.
- W2070600324 cites W2134045039 @default.
- W2070600324 cites W2136185033 @default.
- W2070600324 cites W2140807026 @default.
- W2070600324 cites W2150373282 @default.
- W2070600324 cites W2152032405 @default.
- W2070600324 cites W2153773005 @default.
- W2070600324 cites W2155158652 @default.
- W2070600324 cites W2156689536 @default.