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- W2070639142 abstract "Uropathogenic Escherichia coli express pili that mediate binding to host tissue cells. We demonstrate with in situ force measuring optical tweezers that the ability of P and type 1 pili to elongate by unfolding under exposure to stress is a shared property with some differences. The unfolding force of the quaternary structures under equilibrium conditions is similar, 28 ± 2 and 30 ± 2 pN for P pili and type 1 pili, respectively. However, type 1 pili are found to be more rigid than P pili through their stronger layer-to-layer bonds. It was found that type 1 pili enter a dynamic regime at elongation speeds of 6 nm/s, compared to 400 nm/s for P pili; i.e., it responds faster to an external force. This possibly helps type 1 to withstand the irregular urine flow in the urethra as compared to the more constant urine flow in the upper urinary tract. Also, it was found that type 1 pili refold during retraction at two different levels that possibly could be related to several possible configurations. Our findings highlight functions that are believed to be of importance for the bacterial ability to sustain a basic antimicrobial mechanism of the host and for bacterial colonization." @default.
- W2070639142 created "2016-06-24" @default.
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- W2070639142 date "2007-11-01" @default.
- W2070639142 modified "2023-09-27" @default.
- W2070639142 title "The Biomechanical Properties of E. coli Pili for Urinary Tract Attachment Reflect the Host Environment" @default.
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- W2070639142 doi "https://doi.org/10.1529/biophysj.107.110643" @default.
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