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- W2070749071 abstract "Constitutive membrane trafficking events are regulated by heterotrimeric G-proteins (G-proteins) in addition to their regulation by small GTP-binding proteins (smgs). Here, we used streptolysin O-permeabilized mouse pancreatic acini and compounds that interact with G-proteins, but not smgs, to examine whether G-proteins are also involved in regulated pancreatic exocytosis. The wasp venom mastoparan (10 μM inhibited by 25–50% amylase release from permeabilized acini stimulated by various combinations of Ca2+, cyclic AMP (cAMP), 12-O-tetradecanoylphorbol 13-acetate, and guanosine (5'-[γ-thio]triphosphate (GTPγS), while the inactive analogue Mas17 was without effect. Pretreatment of intact acini with pertussis toxin resulted in an ∼30% reduction of amylase secretion from cells subsequently permeabilized and stimulated with calcium and GTPγS. Pretreatment of intact acini with cholera toxin increased stimulated amylase release by 30% from subsequently permeabilized cells, and this effect was mimicked by 8-Br-cAMP. The cAMP-dependent protein kinase inhibitor H-89 (3 μM) largely reversed the effect of cholera toxin, indicating that cholera toxin's effect is due to increased cellular cAMP levels. The inhibitory effects of mastoparan and pertussis toxin suggest that a Gi/Go-type G-protein(s) is (are) involved in the regulation of exocytosis. Since mastoparan inhibited exocytosis stimulated by all intracellular mediators tested, it indicates that the G-protein acts at a distal step in the exocytic process." @default.
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- W2070749071 date "1995-05-01" @default.
- W2070749071 modified "2023-10-12" @default.
- W2070749071 title "Evidence of Heterotrimeric G-Protein Involvement in Regulated Exocytosis from Permeabilized Pancreatic Acini" @default.
- W2070749071 doi "https://doi.org/10.1097/00006676-199505000-00009" @default.
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