Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070797966> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2070797966 endingPage "108" @default.
- W2070797966 startingPage "100" @default.
- W2070797966 abstract "Synovial tissue is rarely available from patients with early synovitis, with the exception of synovial biopsies. However, T cell populations early in the development of synovitis may be enriched in antigen-specific cells and critical to disease pathogenesis. To investigate the T cell repertoire in early synovitis, we utilized a PCR protocol for detection of T cell receptor (TCR) transcripts present in small amounts of synovial tissue. To expand the substrate for PCR, preamplification of cDNA was performed with a 3' constant region primer plus either a mixture of variable region primers or random hexanucleotides. Utilizing this method improved the sensitivity of detection. This technique is applied here to the analysis of TCR transcripts in synovial biopsies from individuals with early rheumatoid arthritis (RA) and non-RA synovitis. TCR alpha-chain transcripts were detectable in 5/5 RA and 4/4 non-RA specimens evaluated, with beta-chain transcripts detected in 4/5 early RA and 4/4 non-RA specimens evaluated. Confirmation of transcripts by sequencing of cloned PCR products verified the specificity of amplification. The most frequently expressed TCR V region families in early RA synovitis were V alpha 11, V alpha 14, V alpha 28, V beta 7, V beta 9 and V beta 17. Several of these V regions have previously been implicated in studies of chronic RA synovitis. J alpha and J beta region usage was similar to that seen in chronic RA, and conserved N region motifs were apparent. We conclude that it is possible to detect TCR transcripts in small synovial biopsies from individuals with early arthritis.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2070797966 created "2016-06-24" @default.
- W2070797966 creator A5006951712 @default.
- W2070797966 creator A5009445173 @default.
- W2070797966 creator A5019900494 @default.
- W2070797966 creator A5029796262 @default.
- W2070797966 creator A5029973420 @default.
- W2070797966 creator A5054041214 @default.
- W2070797966 creator A5057036371 @default.
- W2070797966 creator A5076651401 @default.
- W2070797966 creator A5080816215 @default.
- W2070797966 creator A5086946489 @default.
- W2070797966 date "1995-01-01" @default.
- W2070797966 modified "2023-10-16" @default.
- W2070797966 title "Detection of T Cell Receptors in Early Rheumatoid Arthritis Synovial Tissue" @default.
- W2070797966 doi "https://doi.org/10.1159/000163940" @default.
- W2070797966 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8554698" @default.
- W2070797966 hasPublicationYear "1995" @default.
- W2070797966 type Work @default.
- W2070797966 sameAs 2070797966 @default.
- W2070797966 citedByCount "8" @default.
- W2070797966 countsByYear W20707979662013 @default.
- W2070797966 crossrefType "journal-article" @default.
- W2070797966 hasAuthorship W2070797966A5006951712 @default.
- W2070797966 hasAuthorship W2070797966A5009445173 @default.
- W2070797966 hasAuthorship W2070797966A5019900494 @default.
- W2070797966 hasAuthorship W2070797966A5029796262 @default.
- W2070797966 hasAuthorship W2070797966A5029973420 @default.
- W2070797966 hasAuthorship W2070797966A5054041214 @default.
- W2070797966 hasAuthorship W2070797966A5057036371 @default.
- W2070797966 hasAuthorship W2070797966A5076651401 @default.
- W2070797966 hasAuthorship W2070797966A5080816215 @default.
- W2070797966 hasAuthorship W2070797966A5086946489 @default.
- W2070797966 hasConcept C104317684 @default.
- W2070797966 hasConcept C142724271 @default.
- W2070797966 hasConcept C153911025 @default.
- W2070797966 hasConcept C178790620 @default.
- W2070797966 hasConcept C185592680 @default.
- W2070797966 hasConcept C187882448 @default.
- W2070797966 hasConcept C19317047 @default.
- W2070797966 hasConcept C203014093 @default.
- W2070797966 hasConcept C204787440 @default.
- W2070797966 hasConcept C2776090121 @default.
- W2070797966 hasConcept C2776164576 @default.
- W2070797966 hasConcept C2776268809 @default.
- W2070797966 hasConcept C2777563447 @default.
- W2070797966 hasConcept C2777575956 @default.
- W2070797966 hasConcept C2777652487 @default.
- W2070797966 hasConcept C2779244835 @default.
- W2070797966 hasConcept C2780942790 @default.
- W2070797966 hasConcept C54355233 @default.
- W2070797966 hasConcept C71924100 @default.
- W2070797966 hasConcept C86803240 @default.
- W2070797966 hasConcept C8891405 @default.
- W2070797966 hasConceptScore W2070797966C104317684 @default.
- W2070797966 hasConceptScore W2070797966C142724271 @default.
- W2070797966 hasConceptScore W2070797966C153911025 @default.
- W2070797966 hasConceptScore W2070797966C178790620 @default.
- W2070797966 hasConceptScore W2070797966C185592680 @default.
- W2070797966 hasConceptScore W2070797966C187882448 @default.
- W2070797966 hasConceptScore W2070797966C19317047 @default.
- W2070797966 hasConceptScore W2070797966C203014093 @default.
- W2070797966 hasConceptScore W2070797966C204787440 @default.
- W2070797966 hasConceptScore W2070797966C2776090121 @default.
- W2070797966 hasConceptScore W2070797966C2776164576 @default.
- W2070797966 hasConceptScore W2070797966C2776268809 @default.
- W2070797966 hasConceptScore W2070797966C2777563447 @default.
- W2070797966 hasConceptScore W2070797966C2777575956 @default.
- W2070797966 hasConceptScore W2070797966C2777652487 @default.
- W2070797966 hasConceptScore W2070797966C2779244835 @default.
- W2070797966 hasConceptScore W2070797966C2780942790 @default.
- W2070797966 hasConceptScore W2070797966C54355233 @default.
- W2070797966 hasConceptScore W2070797966C71924100 @default.
- W2070797966 hasConceptScore W2070797966C86803240 @default.
- W2070797966 hasConceptScore W2070797966C8891405 @default.
- W2070797966 hasIssue "2" @default.
- W2070797966 hasLocation W20707979661 @default.
- W2070797966 hasLocation W20707979662 @default.
- W2070797966 hasOpenAccess W2070797966 @default.
- W2070797966 hasPrimaryLocation W20707979661 @default.
- W2070797966 hasRelatedWork W1979499339 @default.
- W2070797966 hasRelatedWork W2021299421 @default.
- W2070797966 hasRelatedWork W2095011332 @default.
- W2070797966 hasRelatedWork W2111012346 @default.
- W2070797966 hasRelatedWork W2118897875 @default.
- W2070797966 hasRelatedWork W2369375039 @default.
- W2070797966 hasRelatedWork W2370293128 @default.
- W2070797966 hasRelatedWork W2373507912 @default.
- W2070797966 hasRelatedWork W2417962227 @default.
- W2070797966 hasRelatedWork W330405577 @default.
- W2070797966 hasVolume "63" @default.
- W2070797966 isParatext "false" @default.
- W2070797966 isRetracted "false" @default.
- W2070797966 magId "2070797966" @default.
- W2070797966 workType "article" @default.