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• W2070798950 abstract "Xenopus oocytes injected with human U2 snRNA genes synthesize mature U2 as well as a U2 precursor with about 10 extra 3′ nucleotides (human pre-U2 RNA). Formation of the pre-U2 3' end requires a downstream element located between position +16 and +37 in the U2 3′-flanking sequence. The distance between this element and the U2 coding region can be increased without affecting formation of the pre-U2 3′ end. When the natural sequence surrounding the pre-U2 3′ end is changed, novel 3′ ends are still generated within a narrow range upstream from the element. The 3′ terminal stem-loop of U2 snRNA is not required for pre-U2 3′ end formation. A sequence within the 3′ element (GTTTN0-3AAAPuNNAGA) is conserved among snRNA genes transcribed by RNA polymerase II. Our results suggest that the 3′ ends of pre-U2 RNA and histone mRNA may be generated by related but distinct RNA processing mechanisms. Xenopus oocytes injected with human U2 snRNA genes synthesize mature U2 as well as a U2 precursor with about 10 extra 3′ nucleotides (human pre-U2 RNA). Formation of the pre-U2 3' end requires a downstream element located between position +16 and +37 in the U2 3′-flanking sequence. The distance between this element and the U2 coding region can be increased without affecting formation of the pre-U2 3′ end. When the natural sequence surrounding the pre-U2 3′ end is changed, novel 3′ ends are still generated within a narrow range upstream from the element. The 3′ terminal stem-loop of U2 snRNA is not required for pre-U2 3′ end formation. A sequence within the 3′ element (GTTTN0-3AAAPuNNAGA) is conserved among snRNA genes transcribed by RNA polymerase II. Our results suggest that the 3′ ends of pre-U2 RNA and histone mRNA may be generated by related but distinct RNA processing mechanisms." @default.
• W2070798950 created "2016-06-24" @default.
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• W2070798950 date "1985-08-01" @default.
• W2070798950 modified "2023-10-14" @default.
• W2070798950 title "Sequences required for 3′ end formation of human U2 small nuclear RNA" @default.
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• W2070798950 doi "https://doi.org/10.1016/s0092-8674(85)80115-x" @default.
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