FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2070862082> ?p ?o ?g. }

• W2070862082 endingPage "394" @default.
• W2070862082 startingPage "384" @default.
• W2070862082 abstract "GlcN(acyl)PtdIns, a derivative of phosphatidylinositol (PtdIns) in which glucosamine and a fatty acid are linked to inositol hydroxyl groups, has been proposed to be an intermediate in the mammalian biosynthetic pathway for glycosylphosphatidylinositol (glycosyl-PtdIns) anchors of membrane proteins. In this report, GlcN(acyl)PtdIns metabolically labeled with [3H]inositol is shown to accumulate in a HeLa S3 cell subline. The amount of GlcN(acyl)PtdIns in these HeLa S3 cells is about 10(7) molecules/cell, a level comparable to those of the most abundant glycosyl-PtdIns-containing molecules reported to date. GlcN(acyl)PtdIns was purified by a two-step procedure involving octyl-Sepharose and thin-layer chromatography. Octyl-Sepharose separated phospholipids according to their number of hydrocarbon chains: one in 2-lysoPtdIns, two in PtdIns, and three in GlcN(acyl)PtdIns. Purification also was aided by prior treatment of lipid extracts with bee venom phospholipase A2, an enzyme that did not cleave GlcN(acyl)PtdIns. The GlcN-inositol head group in purified GlcN(acyl)PtdIns was confirmed by a number of procedures, including cation-exchange chromatography and mass spectrometry; after radiomethylation, an equal molar ratio of GlcN(Me)2/inositol was measured. Fatty acid analysis indicated an overall stoichiometry of 2.3 mol fatty acid/mol inositol with palmitic (16:0), stearic (18:0) and oleic (18:1) acids being predominant. Analysis of GlcN(acyl)inositol produced by HF fragmentation showed that palmitate was the acyl group attached to inositol and indicated that stearic and oleic acids were in the glycerolipid. Base methanolysis revealed that about 15% of the purified GlcN(acyl)PtdIns contained alkylglycerol. A substantial conversion of GlcN(acyl)PtdIns to a slightly more polar lipid occurred after overnight incubation in even mildly alkaline buffers. Although the current data do not allow proposal of a structure for this lipid, its formation from GlcN(acyl)PtdIns may be important because the conversion appeared to occur in vivo." @default.
• W2070862082 created "2016-06-24" @default.
• W2070862082 creator A5002695228 @default.
• W2070862082 creator A5014699018 @default.
• W2070862082 creator A5021659131 @default.
• W2070862082 date "1995-10-01" @default.
• W2070862082 modified "2023-09-30" @default.
• W2070862082 title "Compositional analysis of Glucosaminyl(acyl)phosphatidylinositol Accumulated in HeLa S3 Cells" @default.
• W2070862082 cites W1480176123 @default.
• W2070862082 cites W1482241944 @default.
• W2070862082 cites W1484518952 @default.
• W2070862082 cites W1485988308 @default.
• W2070862082 cites W1487665505 @default.
• W2070862082 cites W1492544808 @default.
• W2070862082 cites W1493219418 @default.
• W2070862082 cites W1494639580 @default.
• W2070862082 cites W1508389393 @default.
• W2070862082 cites W1532360582 @default.
• W2070862082 cites W1536399816 @default.
• W2070862082 cites W1538608251 @default.
• W2070862082 cites W1543271228 @default.
• W2070862082 cites W1543999675 @default.
• W2070862082 cites W1549823484 @default.
• W2070862082 cites W1550837995 @default.
• W2070862082 cites W1554779196 @default.
• W2070862082 cites W1557890613 @default.
• W2070862082 cites W1562203178 @default.
• W2070862082 cites W1568628251 @default.
• W2070862082 cites W1571485829 @default.
• W2070862082 cites W1583769399 @default.
• W2070862082 cites W1642077255 @default.
• W2070862082 cites W1738235581 @default.
• W2070862082 cites W1767192041 @default.
• W2070862082 cites W1884967233 @default.
• W2070862082 cites W1912410317 @default.
• W2070862082 cites W1943630073 @default.
• W2070862082 cites W1944304173 @default.
• W2070862082 cites W1949772429 @default.
• W2070862082 cites W1982095481 @default.
• W2070862082 cites W1994238274 @default.
• W2070862082 cites W2018164924 @default.
• W2070862082 cites W2045136758 @default.
• W2070862082 cites W2047831885 @default.
• W2070862082 cites W2051441654 @default.
• W2070862082 cites W2053434732 @default.
• W2070862082 cites W2057007315 @default.
• W2070862082 cites W2063287165 @default.
• W2070862082 cites W2065698593 @default.
• W2070862082 cites W2068496808 @default.
• W2070862082 cites W2080464418 @default.
• W2070862082 cites W2100285359 @default.
• W2070862082 cites W2110403618 @default.
• W2070862082 cites W2135556297 @default.
• W2070862082 cites W4232828420 @default.
• W2070862082 cites W4254625887 @default.
• W2070862082 doi "https://doi.org/10.1111/j.1432-1033.1995.384_1.x" @default.
• W2070862082 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7588771" @default.
• W2070862082 hasPublicationYear "1995" @default.
• W2070862082 type Work @default.
• W2070862082 sameAs 2070862082 @default.
• W2070862082 citedByCount "23" @default.
• W2070862082 countsByYear W20708620822021 @default.
• W2070862082 crossrefType "journal-article" @default.
• W2070862082 hasAuthorship W2070862082A5002695228 @default.
• W2070862082 hasAuthorship W2070862082A5014699018 @default.
• W2070862082 hasAuthorship W2070862082A5021659131 @default.
• W2070862082 hasBestOaLocation W20708620821 @default.
• W2070862082 hasConcept C12927208 @default.
• W2070862082 hasConcept C1491633281 @default.
• W2070862082 hasConcept C161790260 @default.
• W2070862082 hasConcept C170493617 @default.
• W2070862082 hasConcept C178790620 @default.
• W2070862082 hasConcept C181199279 @default.
• W2070862082 hasConcept C185592680 @default.
• W2070862082 hasConcept C193557335 @default.
• W2070862082 hasConcept C2777366897 @default.
• W2070862082 hasConcept C2777427919 @default.
• W2070862082 hasConcept C2778125326 @default.
• W2070862082 hasConcept C2778597717 @default.
• W2070862082 hasConcept C2778918659 @default.
• W2070862082 hasConcept C2779516699 @default.
• W2070862082 hasConcept C2780263894 @default.
• W2070862082 hasConcept C2780610907 @default.
• W2070862082 hasConcept C2780743229 @default.
• W2070862082 hasConcept C41625074 @default.
• W2070862082 hasConcept C543025807 @default.
• W2070862082 hasConcept C55493867 @default.
• W2070862082 hasConcept C62478195 @default.
• W2070862082 hasConcept C71240020 @default.
• W2070862082 hasConceptScore W2070862082C12927208 @default.
• W2070862082 hasConceptScore W2070862082C1491633281 @default.
• W2070862082 hasConceptScore W2070862082C161790260 @default.
• W2070862082 hasConceptScore W2070862082C170493617 @default.
• W2070862082 hasConceptScore W2070862082C178790620 @default.
• W2070862082 hasConceptScore W2070862082C181199279 @default.
• W2070862082 hasConceptScore W2070862082C185592680 @default.
• W2070862082 hasConceptScore W2070862082C193557335 @default.
• W2070862082 hasConceptScore W2070862082C2777366897 @default.

• next