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- W2070955173 abstract "Agnoprotein encoded by human polyomavirus BK (BKV) is a late cytoplasmic protein of 66 amino acids (aa) of unknown function. Immunofluorescence microscopy revealed a fine granular and a vesicular distribution in donut-like structures. Using BKV(Dunlop)-infected or agnoprotein-transfected cells, we investigated agnoprotein co-localization with subcellular structures. We found that agnoprotein co-localizes with lipid droplets (LD) in primary human renal tubular epithelial cells as well as in other cells supporting BKV replication in vitro (UTA, Vero cells). Using agnoprotein-enhanced green fluorescent protein (EGFP) fusion constructs, we demonstrate that agnoprotein aa 20–42 are required for targeting LD, whereas aa 1–20 or aa 42–66 were not. Agnoprotein aa 22–40 are predicted to form an amphipathic helix, and mutations A25D and F39E, disrupting its hydrophobic domain, prevented LD targeting. However, changing the phosphorylation site serine-11 to alanine or aspartic acid did not alter LD co-localization. Our findings provide new clues to unravel agnoprotein function." @default.
- W2070955173 created "2016-06-24" @default.
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- W2070955173 date "2010-04-01" @default.
- W2070955173 modified "2023-10-06" @default.
- W2070955173 title "The polyomavirus BK agnoprotein co-localizes with lipid droplets" @default.
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- W2070955173 doi "https://doi.org/10.1016/j.virol.2010.01.011" @default.
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