Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071027165> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2071027165 endingPage "459" @default.
- W2071027165 startingPage "453" @default.
- W2071027165 abstract "Glia-derived nexin (GDN) is a 43-kDa glycoprotein isolated from rat glioma cell cultures. It promotes neurite extension in cultures of neuroblastoma cells and chick sympathetic neurons. Moreover, GDN is a potent serine protease inhibitor (serpin), belonging to the family of protease nexins. We report here the expression of rat GDN in the Saccharomyces cerevisiae strain GRF18 under the control of the PHO5 promoter. We describe the purification of more than 6 mg total GDN from the cellular extract of 1 liter of yeast culture. The amino acid composition and the sequence of CNBr-fragments of the recombinant protein correlate with the values deduced from the rat GDN cDNA. We provide evidence that the recombinant GDN has exactly the same properties as the glioma-derived protein with respect to its protease-inhibitory activity and its ability to promote the extension of neurites from neuroblastoma cells. The large amounts of recombinant protein obtained from this expression system will allow further biochemical and physiological analysis of GDN and of the serpins in general." @default.
- W2071027165 created "2016-06-24" @default.
- W2071027165 creator A5015924778 @default.
- W2071027165 creator A5023462355 @default.
- W2071027165 creator A5034069386 @default.
- W2071027165 creator A5061211776 @default.
- W2071027165 creator A5076408948 @default.
- W2071027165 date "1989-12-01" @default.
- W2071027165 modified "2023-09-27" @default.
- W2071027165 title "Synthesis of glia-derived nexin in yeast" @default.
- W2071027165 cites W1463270837 @default.
- W2071027165 cites W1520082587 @default.
- W2071027165 cites W1571398079 @default.
- W2071027165 cites W168949879 @default.
- W2071027165 cites W1910136064 @default.
- W2071027165 cites W1969696437 @default.
- W2071027165 cites W2002471001 @default.
- W2071027165 cites W2003117013 @default.
- W2071027165 cites W2005881556 @default.
- W2071027165 cites W2007672783 @default.
- W2071027165 cites W2014669590 @default.
- W2071027165 cites W2016432377 @default.
- W2071027165 cites W2025591509 @default.
- W2071027165 cites W2033964802 @default.
- W2071027165 cites W2063450941 @default.
- W2071027165 cites W2071339552 @default.
- W2071027165 cites W2083968879 @default.
- W2071027165 cites W2085625230 @default.
- W2071027165 cites W2091437216 @default.
- W2071027165 cites W2100837269 @default.
- W2071027165 doi "https://doi.org/10.1016/0378-1119(89)90439-3" @default.
- W2071027165 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2697643" @default.
- W2071027165 hasPublicationYear "1989" @default.
- W2071027165 type Work @default.
- W2071027165 sameAs 2071027165 @default.
- W2071027165 citedByCount "28" @default.
- W2071027165 countsByYear W20710271652016 @default.
- W2071027165 crossrefType "journal-article" @default.
- W2071027165 hasAuthorship W2071027165A5015924778 @default.
- W2071027165 hasAuthorship W2071027165A5023462355 @default.
- W2071027165 hasAuthorship W2071027165A5034069386 @default.
- W2071027165 hasAuthorship W2071027165A5061211776 @default.
- W2071027165 hasAuthorship W2071027165A5076408948 @default.
- W2071027165 hasConcept C104317684 @default.
- W2071027165 hasConcept C113246987 @default.
- W2071027165 hasConcept C153911025 @default.
- W2071027165 hasConcept C181199279 @default.
- W2071027165 hasConcept C187882448 @default.
- W2071027165 hasConcept C202751555 @default.
- W2071027165 hasConcept C2776714187 @default.
- W2071027165 hasConcept C2777576037 @default.
- W2071027165 hasConcept C2777807008 @default.
- W2071027165 hasConcept C2778965386 @default.
- W2071027165 hasConcept C2779222958 @default.
- W2071027165 hasConcept C40767141 @default.
- W2071027165 hasConcept C55493867 @default.
- W2071027165 hasConcept C86803240 @default.
- W2071027165 hasConceptScore W2071027165C104317684 @default.
- W2071027165 hasConceptScore W2071027165C113246987 @default.
- W2071027165 hasConceptScore W2071027165C153911025 @default.
- W2071027165 hasConceptScore W2071027165C181199279 @default.
- W2071027165 hasConceptScore W2071027165C187882448 @default.
- W2071027165 hasConceptScore W2071027165C202751555 @default.
- W2071027165 hasConceptScore W2071027165C2776714187 @default.
- W2071027165 hasConceptScore W2071027165C2777576037 @default.
- W2071027165 hasConceptScore W2071027165C2777807008 @default.
- W2071027165 hasConceptScore W2071027165C2778965386 @default.
- W2071027165 hasConceptScore W2071027165C2779222958 @default.
- W2071027165 hasConceptScore W2071027165C40767141 @default.
- W2071027165 hasConceptScore W2071027165C55493867 @default.
- W2071027165 hasConceptScore W2071027165C86803240 @default.
- W2071027165 hasIssue "2" @default.
- W2071027165 hasLocation W20710271651 @default.
- W2071027165 hasLocation W20710271652 @default.
- W2071027165 hasOpenAccess W2071027165 @default.
- W2071027165 hasPrimaryLocation W20710271651 @default.
- W2071027165 hasRelatedWork W1988507202 @default.
- W2071027165 hasRelatedWork W2338137066 @default.
- W2071027165 hasRelatedWork W2350146650 @default.
- W2071027165 hasRelatedWork W2363249581 @default.
- W2071027165 hasRelatedWork W2373690171 @default.
- W2071027165 hasRelatedWork W2376921901 @default.
- W2071027165 hasRelatedWork W2379425332 @default.
- W2071027165 hasRelatedWork W2382285667 @default.
- W2071027165 hasRelatedWork W2386419125 @default.
- W2071027165 hasRelatedWork W2413314792 @default.
- W2071027165 hasVolume "85" @default.
- W2071027165 isParatext "false" @default.
- W2071027165 isRetracted "false" @default.
- W2071027165 magId "2071027165" @default.
- W2071027165 workType "article" @default.