FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071094953> ?p ?o ?g. }

• W2071094953 endingPage "242" @default.
• W2071094953 startingPage "227" @default.
• W2071094953 abstract "Ribosomal RNA biosynthesis in metabolically active spheroplasts of the yeast Saccharomyces cerevisiae was selected as a model system for the study of nucleo-cytoplasmic interactions. Examination of the total cellular RNA synthesized during short exposures to labeled nucleosides demonstrated the existence of three large, short-lived RNA species of 35 s, 27 s and 20 s, in addition to the mature ribosomal RNA's, 25 s and 18 s. Little or no RNA larger than 35 s is found. The kinetics of synthesis of these RNA molecules was pursued by continuous labeling and pulse-chase experiments, using [methyl-3H]methionine to label only ribosomal species. These results demonstrated the following precursor-product relationship: In the presence of cycloheximide the terminal processing steps leading to the mature ribosomal RNA's are severely inhibited, indicating that the maturation of ribosomal precursor RNA requires continuous protein synthesis. RNA denaturation studies using dimethyl sulfoxide showed the precursors to be single polyribonucleotide chains larger than the mature rRNA's, rather than aggregates of smaller molecules, or conformational isomers of the ribosomal species. Molecular sizes of these RNA's were estimated by electrophoretic mobility in acrylamide gels. The 35 s precursor is 2.5 × 106 daltons. Its cleavage appears to be conservative, producing intermediate molecules of 1.6 × 106 and 0.8 × 106 daltons. The final maturation steps yield mature rRNA's of 1.3 × 106 and 0.7 × 106 daltons with the concomitant loss of about 20% of the original precursor molecule. In addition, it was concluded that the small RNA species, 5.8 s (6 × 104 daltons) found non-covalently bound to the 25 s RNA, is generated during the final cleavage of the 27 s precursor molecule. In contrast, 5 s RNA is synthesized independently. These results support the contention that all eukaryotes synthesize ribosomes similarly and that yeast may be a useful organism for the investigation of intracellular communication, particularly across the nuclear membrane." @default.
• W2071094953 created "2016-06-24" @default.
• W2071094953 creator A5041733646 @default.
• W2071094953 creator A5054693718 @default.
• W2071094953 date "1972-03-01" @default.
• W2071094953 modified "2023-09-25" @default.
• W2071094953 title "Ribosomal RNA synthesis in Saccharomyces cerevisiae" @default.
• W2071094953 cites W1490179123 @default.
• W2071094953 cites W1550374375 @default.
• W2071094953 cites W1565339037 @default.
• W2071094953 cites W1603236137 @default.
• W2071094953 cites W1607813503 @default.
• W2071094953 cites W1808632021 @default.
• W2071094953 cites W1811297709 @default.
• W2071094953 cites W1833411966 @default.
• W2071094953 cites W1841036831 @default.
• W2071094953 cites W1907745154 @default.
• W2071094953 cites W1936762528 @default.
• W2071094953 cites W1976497610 @default.
• W2071094953 cites W1993749032 @default.
• W2071094953 cites W1999064404 @default.
• W2071094953 cites W1999292075 @default.
• W2071094953 cites W2000212207 @default.
• W2071094953 cites W2020090524 @default.
• W2071094953 cites W2023227497 @default.
• W2071094953 cites W2024622869 @default.
• W2071094953 cites W2031167587 @default.
• W2071094953 cites W2033276522 @default.
• W2071094953 cites W2037120427 @default.
• W2071094953 cites W2037198776 @default.
• W2071094953 cites W2041491250 @default.
• W2071094953 cites W2057370817 @default.
• W2071094953 cites W2057434874 @default.
• W2071094953 cites W2066121359 @default.
• W2071094953 cites W2076343846 @default.
• W2071094953 cites W2083785398 @default.
• W2071094953 cites W2090525002 @default.
• W2071094953 cites W2153184117 @default.
• W2071094953 cites W2240726600 @default.
• W2071094953 cites W2284872392 @default.
• W2071094953 cites W2396891102 @default.
• W2071094953 doi "https://doi.org/10.1016/0022-2836(72)90279-3" @default.
• W2071094953 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4557192" @default.
• W2071094953 hasPublicationYear "1972" @default.
• W2071094953 type Work @default.
• W2071094953 sameAs 2071094953 @default.
• W2071094953 citedByCount "357" @default.
• W2071094953 countsByYear W20710949532012 @default.
• W2071094953 countsByYear W20710949532013 @default.
• W2071094953 countsByYear W20710949532014 @default.
• W2071094953 countsByYear W20710949532015 @default.
• W2071094953 countsByYear W20710949532016 @default.
• W2071094953 countsByYear W20710949532017 @default.
• W2071094953 countsByYear W20710949532018 @default.
• W2071094953 countsByYear W20710949532019 @default.
• W2071094953 countsByYear W20710949532021 @default.
• W2071094953 countsByYear W20710949532022 @default.
• W2071094953 countsByYear W20710949532023 @default.
• W2071094953 crossrefType "journal-article" @default.
• W2071094953 hasAuthorship W2071094953A5041733646 @default.
• W2071094953 hasAuthorship W2071094953A5054693718 @default.
• W2071094953 hasConcept C104317684 @default.
• W2071094953 hasConcept C1248232 @default.
• W2071094953 hasConcept C153911025 @default.
• W2071094953 hasConcept C153957851 @default.
• W2071094953 hasConcept C185592680 @default.
• W2071094953 hasConcept C25281209 @default.
• W2071094953 hasConcept C2777576037 @default.
• W2071094953 hasConcept C2779222958 @default.
• W2071094953 hasConcept C41258723 @default.
• W2071094953 hasConcept C55493867 @default.
• W2071094953 hasConcept C67705224 @default.
• W2071094953 hasConcept C67905577 @default.
• W2071094953 hasConcept C86803240 @default.
• W2071094953 hasConcept C88478588 @default.
• W2071094953 hasConcept C98098607 @default.
• W2071094953 hasConceptScore W2071094953C104317684 @default.
• W2071094953 hasConceptScore W2071094953C1248232 @default.
• W2071094953 hasConceptScore W2071094953C153911025 @default.
• W2071094953 hasConceptScore W2071094953C153957851 @default.
• W2071094953 hasConceptScore W2071094953C185592680 @default.
• W2071094953 hasConceptScore W2071094953C25281209 @default.
• W2071094953 hasConceptScore W2071094953C2777576037 @default.
• W2071094953 hasConceptScore W2071094953C2779222958 @default.
• W2071094953 hasConceptScore W2071094953C41258723 @default.
• W2071094953 hasConceptScore W2071094953C55493867 @default.
• W2071094953 hasConceptScore W2071094953C67705224 @default.
• W2071094953 hasConceptScore W2071094953C67905577 @default.
• W2071094953 hasConceptScore W2071094953C86803240 @default.
• W2071094953 hasConceptScore W2071094953C88478588 @default.
• W2071094953 hasConceptScore W2071094953C98098607 @default.
• W2071094953 hasIssue "2" @default.
• W2071094953 hasLocation W20710949531 @default.
• W2071094953 hasLocation W20710949532 @default.
• W2071094953 hasOpenAccess W2071094953 @default.
• W2071094953 hasPrimaryLocation W20710949531 @default.
• W2071094953 hasRelatedWork W1607813503 @default.
• W2071094953 hasRelatedWork W2001657242 @default.

• next