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• W2071161992 abstract "Semiconducting nanoparticle ion-sensitive field-effect transistors (ISFETs) are used to detect immunoglobulin G (IgG) in the conventional enzyme-linked immunosorbent assay (ELISA). Indium oxide and silica nanoparticles were layer-by-layer self-assembled with the oppositely charged polyelectrolyte as the electrochemical transducer and antibody immobilization site, respectively. The assay was conducted on a novel platform of indium oxide nanoparticle ISFETs, where the electric signals are generated in response to the concentration of target IgG using the labeled detecting antibody. The sandwiched ELISA structure catalyzed the conversion of the acidic substrate into neutral substance with the aid of horseradish peroxidase. The pH change in the substrate solution was detected by nanoparticle ISFETs. Normal rabbit IgG was used as a model antigen whose detection limit of 0.04 ng ml−1 was found. The facile electric detection in the conventional assay through the semiconducting nanoparticle ISFET has potential applications as a point-of-care detection or a sensing element in a lab-on-a-chip system." @default.
• W2071161992 created "2016-06-24" @default.
• W2071161992 creator A5018478717 @default.
• W2071161992 creator A5072415165 @default.
• W2071161992 date "2011-12-20" @default.
• W2071161992 modified "2023-09-27" @default.
• W2071161992 title "An electric detection of immunoglobulin G in the enzyme-linked immunosorbent assay using an indium oxide nanoparticle ion-sensitive field-effect transistor" @default.
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• W2071161992 doi "https://doi.org/10.1088/0960-1317/22/1/015009" @default.
• W2071161992 hasPublicationYear "2011" @default.
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