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• W2071170016 abstract "ABSTRACT Hantaan virus (HTNV)-infected Vero E6 cells undergo cell fusion with both infected and uninfected cells under low-pH conditions. Flow cytometry and fluorescence microscopy of HTNV-infected Vero E6 cells showed that envelope glycoproteins (GPs) were located both on the cell surface and in the cytoplasm. Neutralizing monoclonal antibodies (MAbs) against the G1 and G2 envelope GPs inhibited cell fusion, whereas nonneutralizing MAbs against G1 or G2 and MAbs against the nucleocapsid protein (NP) did not. Transfected Vero E6 cells that expressed GPs but not those that expressed NP fused and formed syncytia. These results indicate that HTNV GPs act as fusogens at the cell surface. No fusion activity was observed either in infected Vero cells that were passaged more than 150 times or in BHK-21 cells, although GPs appeared to localize to the cell surface. This variability in fusion induction suggests the involvement of host cell factors in the process of cell membrane fusion." @default.
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• W2071170016 date "2004-10-01" @default.
• W2071170016 modified "2023-10-16" @default.
• W2071170016 title "Cell Fusion Activities of Hantaan Virus Envelope Glycoproteins" @default.
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• W2071170016 doi "https://doi.org/10.1128/jvi.78.19.10776-10782.2004" @default.
• W2071170016 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/516380" @default.
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