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- W2071222747 abstract "A simple, rapid procedure for disrupting nuclei of mammalian cells has been characterized. This procedure involves treating isolated nuclear suspensions with the natural polyanion heparin in 0.125 m sucrose buffered between pH 7.0 and 8.0. The rate and extent of nuclear lysis are dependent upon the ratio of nuclei concentration to heparin concentration. This procedure avoids use of intense mechanical disruption, enzymatic digestion, and high salt concentrations for achieving optimum lysis of the nuclei. This method can also be used for large-scale nuclear membrane preparations." @default.
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- W2071222747 date "1976-09-01" @default.
- W2071222747 modified "2023-10-14" @default.
- W2071222747 title "A rapid method for preparation of nuclear membranes from mammalian cells" @default.
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- W2071222747 doi "https://doi.org/10.1016/0003-2697(76)90079-8" @default.
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