Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071256502> ?p ?o ?g. }
- W2071256502 endingPage "87" @default.
- W2071256502 startingPage "79" @default.
- W2071256502 abstract "Glucose oxidase (GOx) from Penicillium amagasakiense has a higher specific activity than the more commonly studied Aspergillus niger enzyme, and may therefore be preferred in many medical and industrial applications. The enzyme rapidly inactivates on storage at pH 7.0–7.6 at temperatures between 30 and 40 °C. Results of fluorimetry and circular dichroism spectroscopy indicate that GOx inactivation under these conditions is associated with release of the cofactor FAD and molten globule formation, indicated by major loss of tertiary structure but almost complete retention of secondary structure. Inactivation of GOx at pH < 7 leads to precipitation, but at pH ≥ 7 it leads to non-specific formation of small soluble aggregates detectable by PAGE and size-exclusion chromatography (SEC). Inactivation of P. amagasakiense GOx differs from that of A. niger GOx in displaying complete rather than partial retention of secondary structure and in being promoted rather than prevented by NaCl. The contrasting salt effects may reflect differences in the nature of the interface between subunits in the native dimers and/or the quantity of secondary structure loss upon inactivation." @default.
- W2071256502 created "2016-06-24" @default.
- W2071256502 creator A5011743972 @default.
- W2071256502 creator A5049252208 @default.
- W2071256502 creator A5077541639 @default.
- W2071256502 creator A5091184798 @default.
- W2071256502 date "2011-06-01" @default.
- W2071256502 modified "2023-09-27" @default.
- W2071256502 title "The mechanism of inactivation of glucose oxidase from Penicillium amagasakiense under ambient storage conditions" @default.
- W2071256502 cites W114830531 @default.
- W2071256502 cites W1515119897 @default.
- W2071256502 cites W1521958691 @default.
- W2071256502 cites W1963828758 @default.
- W2071256502 cites W1964905166 @default.
- W2071256502 cites W1967681306 @default.
- W2071256502 cites W1971263364 @default.
- W2071256502 cites W1980102925 @default.
- W2071256502 cites W1983315233 @default.
- W2071256502 cites W1984869639 @default.
- W2071256502 cites W1992733327 @default.
- W2071256502 cites W1993032194 @default.
- W2071256502 cites W2018023840 @default.
- W2071256502 cites W2024593390 @default.
- W2071256502 cites W2026096911 @default.
- W2071256502 cites W2028991761 @default.
- W2071256502 cites W2036187082 @default.
- W2071256502 cites W2045050777 @default.
- W2071256502 cites W2046153401 @default.
- W2071256502 cites W2049174303 @default.
- W2071256502 cites W2056871617 @default.
- W2071256502 cites W2068794270 @default.
- W2071256502 cites W2073756357 @default.
- W2071256502 cites W2076874480 @default.
- W2071256502 cites W2085365687 @default.
- W2071256502 cites W2087401269 @default.
- W2071256502 cites W2089074525 @default.
- W2071256502 cites W2096631030 @default.
- W2071256502 cites W2097100200 @default.
- W2071256502 cites W2104571730 @default.
- W2071256502 cites W2108403767 @default.
- W2071256502 cites W2118830268 @default.
- W2071256502 cites W2129827948 @default.
- W2071256502 cites W2133067061 @default.
- W2071256502 cites W2140260269 @default.
- W2071256502 cites W2142807591 @default.
- W2071256502 cites W2144339897 @default.
- W2071256502 cites W2150809337 @default.
- W2071256502 cites W2172012446 @default.
- W2071256502 cites W2172382055 @default.
- W2071256502 doi "https://doi.org/10.1016/j.enzmictec.2011.03.004" @default.
- W2071256502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22112275" @default.
- W2071256502 hasPublicationYear "2011" @default.
- W2071256502 type Work @default.
- W2071256502 sameAs 2071256502 @default.
- W2071256502 citedByCount "29" @default.
- W2071256502 countsByYear W20712565022012 @default.
- W2071256502 countsByYear W20712565022013 @default.
- W2071256502 countsByYear W20712565022014 @default.
- W2071256502 countsByYear W20712565022015 @default.
- W2071256502 countsByYear W20712565022016 @default.
- W2071256502 countsByYear W20712565022017 @default.
- W2071256502 countsByYear W20712565022018 @default.
- W2071256502 countsByYear W20712565022020 @default.
- W2071256502 countsByYear W20712565022021 @default.
- W2071256502 countsByYear W20712565022022 @default.
- W2071256502 crossrefType "journal-article" @default.
- W2071256502 hasAuthorship W2071256502A5011743972 @default.
- W2071256502 hasAuthorship W2071256502A5049252208 @default.
- W2071256502 hasAuthorship W2071256502A5077541639 @default.
- W2071256502 hasAuthorship W2071256502A5091184798 @default.
- W2071256502 hasConcept C133571119 @default.
- W2071256502 hasConcept C181199279 @default.
- W2071256502 hasConcept C185592680 @default.
- W2071256502 hasConcept C197957613 @default.
- W2071256502 hasConcept C2775960407 @default.
- W2071256502 hasConcept C2777197210 @default.
- W2071256502 hasConcept C43617362 @default.
- W2071256502 hasConcept C55493867 @default.
- W2071256502 hasConcept C62614982 @default.
- W2071256502 hasConceptScore W2071256502C133571119 @default.
- W2071256502 hasConceptScore W2071256502C181199279 @default.
- W2071256502 hasConceptScore W2071256502C185592680 @default.
- W2071256502 hasConceptScore W2071256502C197957613 @default.
- W2071256502 hasConceptScore W2071256502C2775960407 @default.
- W2071256502 hasConceptScore W2071256502C2777197210 @default.
- W2071256502 hasConceptScore W2071256502C43617362 @default.
- W2071256502 hasConceptScore W2071256502C55493867 @default.
- W2071256502 hasConceptScore W2071256502C62614982 @default.
- W2071256502 hasIssue "1" @default.
- W2071256502 hasLocation W20712565021 @default.
- W2071256502 hasLocation W20712565022 @default.
- W2071256502 hasOpenAccess W2071256502 @default.
- W2071256502 hasPrimaryLocation W20712565021 @default.
- W2071256502 hasRelatedWork W1164537646 @default.
- W2071256502 hasRelatedWork W169735115 @default.
- W2071256502 hasRelatedWork W2035731844 @default.
- W2071256502 hasRelatedWork W2071256502 @default.
- W2071256502 hasRelatedWork W2074566080 @default.