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- W2071325233 abstract "Current knowledge of protein-protein interactions in proteome is very limitted due to the lack of high throughput and efficient techniques at the protein level. In this perspective, we briefly discuss the techniques currently available to determine protein-protein interactions and then focus on a novel technique developed by manipulating genome composition in common wheat. Genome manipulation has been carried out either by deleting chromosomes-an arm (ditelocentric lines), a part of chromosome (fine deletion), one of the three genomes, A, B, D-or by introducing a pair of chromosomes (disomic addition line) in the genome composition of common wheat. The network of protein-protein interactions in the wheat seed proteome was determined by quantitative analysis of the expressed proteins. Protein expression in the proteome was found to be controlled by a network. Deletion of chromosome(s), either an arm, a part of an arm or one of the three genomes, has resulted a serious imbalance in the network of protein expression. Addition of extra chromosomes, hence proteins, from the alien species did not affect much to the net work of protein expression. This result indicates that the expression of proteins in proteome is not totally independent, rather there is a network of proteins which determines protein expression in the proteome, and the deletion of chromosome(s), hence proteins, from the network causes a serious irregularities in protein expression as compared to the addition." @default.
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- W2071325233 date "2003-01-01" @default.
- W2071325233 modified "2023-10-16" @default.
- W2071325233 title "Exploring protein-protein interactions in wheat proteome by manipulating genome composition: a perspective" @default.
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- W2071325233 doi "https://doi.org/10.2198/sbk.47.33" @default.
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