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- W2071330500 abstract "A specialized lambda transducing phage carrying the mutH gene and several deletion derivatives of this phage were characterized by restriction enzyme analysis. This analysis localized the mutH gene to a small region of bacterial DNA on the transducing phage and facilitated the subsequent cloning of this gene into the multicopy plasmid pBR322. The mutH gene is contained entirely on a 1.5-kb HindIII fragment as judged by the ability of plasmids carrying this fragment to complement mutH- alleles on the bacterial chromosome. Using recombinant plasmids containing the 1.5-kb HindIII fragment, we identified an Mr 25000 protein as the product of the mutH gene in an in vitro transcription-translation system as well as in maxicells. Various deletion derivatives of the mutH-containing plasmids that exhibit a Mut- phenotype also have lost the Mr 25000 protein." @default.
- W2071330500 created "2016-06-24" @default.
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- W2071330500 date "1983-05-01" @default.
- W2071330500 modified "2023-09-23" @default.
- W2071330500 title "Cloning of mutH and identification of the gene product" @default.
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- W2071330500 doi "https://doi.org/10.1016/0378-1119(83)90109-9" @default.
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