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- W2071333144 abstract "Despite the enormous success of cryocrystallographic techniques, there has been surprisingly little understanding of the fundamental mechanisms relevant to their successful application. I will describe a variety of experiments designed to provide a quantitative, rational basis for cryoprotection and flash cooling [1]. These experiments have resulted in a new approach to cryopreserving protein crystals. Both the cryoprotectant concentrations required to inhibit hexagonal ice formation and the mosaicities of frozen crystals are dramatically reduced over current best practices, simplifying cryoprotectant screening and yielding higher resolution structural information. At the same time, there are many excellent reasons to collect diffraction data at room temperature. Room temperature screening can diagnose problems in as-grown crystal order and in cryoprotective procedures, allowing problem crystals to be weeded out early in the diffraction pipeline. Room temperature may also yield higher resolution structures that more faithfully represent the biologically relevant conformation. I will describe a simple technology [2] that makes room temperature data collection as easy as at low temperatures, and that can be implemented in a high-throughput environment." @default.
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- W2071333144 date "2006-08-06" @default.
- W2071333144 modified "2023-09-27" @default.
- W2071333144 title "New approaches to cryo- and room-temperature crystallography" @default.
- W2071333144 doi "https://doi.org/10.1107/s0108767306099600" @default.
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