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- W2071358260 abstract "We report about the electrocatalytic properties of electrodes modified by adsorption of nitro-fluorenone derivatives. We use their high catalytic activity in the electrooxidation of NADH to enhance the electron transfer kinetics between enzymes of the dehydrogenase family and the electrode. The presence of a carboxyl substituent in the mediator molecule allows us to improve the catalytic rate constants when adding calcium ions to the solution. This is due to a specific complexation between the mediator and NADH. This interaction, as well as the molecular recognition of NADH/NAD+ by dehydrogenases is used to modify the electrode surface in a “molecular lego” approach. The obtained supramolecular sandwich (mediator/Ca2+/NAD+/dehydrogenase) is able to detect specifically the presence of various substrates as a function of the type of dehydrogenase, and constitutes therefore a versatile approach for the construction of biosensors." @default.
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- W2071358260 date "2001-05-01" @default.
- W2071358260 modified "2023-10-10" @default.
- W2071358260 title "Effect of Ca2+ on the Amperometric Determination of Dehydrogenase Substrates with Nitro-Fluorenone Modified Electrodes" @default.
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- W2071358260 doi "https://doi.org/10.1002/1521-4109(200105)13:8/9<770::aid-elan770>3.0.co;2-j" @default.
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