Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071388714> ?p ?o ?g. }
- W2071388714 endingPage "1759" @default.
- W2071388714 startingPage "1753" @default.
- W2071388714 abstract "The immunodepleting effects of alemtuzumab on peripheral blood progenitor cell (PBPC) grafts for stem cell transplantation need to be better defined. The optimal graft cell concentration, antibody dose, need for complement, and whether alemtuzumab is infused with the graft during transplantation remain unclear. PBPC from 6 normal allogeneic stem cell donors harvested by apheresis were first quantitated and the cellular content defined by flow cytometry. Mononuclear cells were then incubated with incremental concentrations of alemtuzumab (.00001, .0001, .001, and .01 mg/mL) for 30 minutes at 20°C or in cell dose responses with 1, 5, and 10 × 10(6) mononuclear cells/mL added to a fixed dose of .001 mg/mL of alemtuzumab with or without a source of complement. Cells were enumerated and analyzed by flow cytometry before and after exposure to alemtuzumab. To determine the presence of unbound anti-CD52, the supernatant of the cell dose responses were tested using the ELISA assay. Selected CD34+ lineage-negative cells were incubated with antibody at the same working concentrations and conditions and cultured in granulocyte-macrophage colony-forming unit assay. The colony numbers were compared with control cultures devoid of the antibody. Incremental concentrations of alemtuzumab led to a significant (2 log) reduction in CD3, CD4, and CD8 populations, which plateaued at .001 mg/mL. Addition of complement led to a further significant reduction in the CD4 and CD8 cells. The maximum CD4 (3 log) and CD8 (2 log) cell death was obtained at 10 × 10(6) cells/mL. Analysis of supernatants for soluble alemtuzumab by ELISA showed a significant reduction in the free antibody concentration when the cell number was increased from 1 to 10 × 10(6) cells/mL implying utilization/binding of the antibody by target cells. Incremental concentrations of alemtuzumab did not affect the number of granulocyte-macrophage colony-forming units. Alemtuzumab depletes all cells expressing the CD52 antigen and has higher activity on CD3, CD8, and particularly on CD4 cells, which are depleted in excess of 2 logs. From this study, we were able to derive that the optimal cell kill in the graft without detectable free alemtuzumab in the supernatant can be achieved with 1 mg of antibody per 100 mL containing 10 × 10(9) cells and active complement (AB serum)." @default.
- W2071388714 created "2016-06-24" @default.
- W2071388714 creator A5025511668 @default.
- W2071388714 creator A5047289156 @default.
- W2071388714 creator A5060292533 @default.
- W2071388714 creator A5070187198 @default.
- W2071388714 date "2013-12-01" @default.
- W2071388714 modified "2023-09-26" @default.
- W2071388714 title "Definition of the Variables Affecting Efficacy of Immunodepletion Ex Vivo of Peripheral Blood Progenitor Cell Grafts by Alemtuzumab (Campath in the Bag)" @default.
- W2071388714 cites W1532162953 @default.
- W2071388714 cites W1972843591 @default.
- W2071388714 cites W1973218385 @default.
- W2071388714 cites W1974997618 @default.
- W2071388714 cites W1981316580 @default.
- W2071388714 cites W1981435011 @default.
- W2071388714 cites W1988239930 @default.
- W2071388714 cites W1989053318 @default.
- W2071388714 cites W2001843840 @default.
- W2071388714 cites W2010450410 @default.
- W2071388714 cites W2021724645 @default.
- W2071388714 cites W2024651597 @default.
- W2071388714 cites W2027732045 @default.
- W2071388714 cites W2029032380 @default.
- W2071388714 cites W2042961427 @default.
- W2071388714 cites W2058483168 @default.
- W2071388714 cites W2072900979 @default.
- W2071388714 cites W2077294419 @default.
- W2071388714 cites W2084177625 @default.
- W2071388714 cites W2087764276 @default.
- W2071388714 cites W2090942656 @default.
- W2071388714 cites W2102505853 @default.
- W2071388714 cites W2140181964 @default.
- W2071388714 cites W2160198948 @default.
- W2071388714 cites W2169182359 @default.
- W2071388714 cites W2325413481 @default.
- W2071388714 cites W83807906 @default.
- W2071388714 doi "https://doi.org/10.1016/j.bbmt.2013.10.001" @default.
- W2071388714 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24120379" @default.
- W2071388714 hasPublicationYear "2013" @default.
- W2071388714 type Work @default.
- W2071388714 sameAs 2071388714 @default.
- W2071388714 citedByCount "3" @default.
- W2071388714 countsByYear W20713887142018 @default.
- W2071388714 countsByYear W20713887142019 @default.
- W2071388714 countsByYear W20713887142021 @default.
- W2071388714 crossrefType "journal-article" @default.
- W2071388714 hasAuthorship W2071388714A5025511668 @default.
- W2071388714 hasAuthorship W2071388714A5047289156 @default.
- W2071388714 hasAuthorship W2071388714A5060292533 @default.
- W2071388714 hasAuthorship W2071388714A5070187198 @default.
- W2071388714 hasBestOaLocation W20713887141 @default.
- W2071388714 hasConcept C10205521 @default.
- W2071388714 hasConcept C126322002 @default.
- W2071388714 hasConcept C137061746 @default.
- W2071388714 hasConcept C150903083 @default.
- W2071388714 hasConcept C153911025 @default.
- W2071388714 hasConcept C159654299 @default.
- W2071388714 hasConcept C16685009 @default.
- W2071388714 hasConcept C167672396 @default.
- W2071388714 hasConcept C201750760 @default.
- W2071388714 hasConcept C202751555 @default.
- W2071388714 hasConcept C203014093 @default.
- W2071388714 hasConcept C207001950 @default.
- W2071388714 hasConcept C26291073 @default.
- W2071388714 hasConcept C2779015954 @default.
- W2071388714 hasConcept C28328180 @default.
- W2071388714 hasConcept C2911091166 @default.
- W2071388714 hasConcept C553184892 @default.
- W2071388714 hasConcept C55493867 @default.
- W2071388714 hasConcept C71924100 @default.
- W2071388714 hasConcept C86803240 @default.
- W2071388714 hasConcept C8891405 @default.
- W2071388714 hasConcept C95444343 @default.
- W2071388714 hasConceptScore W2071388714C10205521 @default.
- W2071388714 hasConceptScore W2071388714C126322002 @default.
- W2071388714 hasConceptScore W2071388714C137061746 @default.
- W2071388714 hasConceptScore W2071388714C150903083 @default.
- W2071388714 hasConceptScore W2071388714C153911025 @default.
- W2071388714 hasConceptScore W2071388714C159654299 @default.
- W2071388714 hasConceptScore W2071388714C16685009 @default.
- W2071388714 hasConceptScore W2071388714C167672396 @default.
- W2071388714 hasConceptScore W2071388714C201750760 @default.
- W2071388714 hasConceptScore W2071388714C202751555 @default.
- W2071388714 hasConceptScore W2071388714C203014093 @default.
- W2071388714 hasConceptScore W2071388714C207001950 @default.
- W2071388714 hasConceptScore W2071388714C26291073 @default.
- W2071388714 hasConceptScore W2071388714C2779015954 @default.
- W2071388714 hasConceptScore W2071388714C28328180 @default.
- W2071388714 hasConceptScore W2071388714C2911091166 @default.
- W2071388714 hasConceptScore W2071388714C553184892 @default.
- W2071388714 hasConceptScore W2071388714C55493867 @default.
- W2071388714 hasConceptScore W2071388714C71924100 @default.
- W2071388714 hasConceptScore W2071388714C86803240 @default.
- W2071388714 hasConceptScore W2071388714C8891405 @default.
- W2071388714 hasConceptScore W2071388714C95444343 @default.
- W2071388714 hasIssue "12" @default.
- W2071388714 hasLocation W20713887141 @default.
- W2071388714 hasLocation W20713887142 @default.