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- W2071433490 abstract "The effect of temperature on Aeromonas hydrophila infection in goldfish, Carassius auratus, was studied using A. hydrophila strain A-3500. After comparison of four different infection methods, subcutaneous injection was selected. Different test temperatures were also tested and higher mortality was observed at 17 and 25°C during a 15-day period. SDS-PAGE analysis of outer membrane proteins prepared from A. hydrophila cultured at 10, 17, 25 and 32°C in formulated salt water showed different protein profiles. For example, a 40-kDa band was found only at 17 and 25°C. Phagocytic rates of A. hydrophila by goldfish macrophages at 10, 17, 25 and 32°C were 20.46 ± 2.07, 16.15 ± 1.39, 15.94 ± 1.85 and 22.22 ± 2.49%, respectively. The results indicated that temperature affects both the cell membrane structure of A. hydrophila and phagocytic activity of goldfish macrophages, resulting in varying fish mortality when infected at different temperatures." @default.
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- W2071433490 date "2001-12-17" @default.
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- W2071433490 title "The effect of temperature on Aeromonas hydrophila infection in goldfish, Carassius auratus" @default.
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- W2071433490 doi "https://doi.org/10.1046/j.1439-0426.2001.00291.x" @default.
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