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• W2071434627 abstract "Vaccination protocols that utilize dendritic cells (DCs) to elicit therapeutic immunity against tumors are the subject of intense research. Given that the capacity of DCs to cross-present antigens is physiologically low, there is considerable interest to develop strategies that enhance that pathway. In order to best exploit the enhanced cross-presentation of antigens bound to heat shock protein 70 (HSP70), we analysed melanoma cell preparations for their HSP70 expression. Western blotting revealed strong upregulation of HSP70 after heat-killing in contrast to UV-B irradiation. When the uptake of heat-killed necrotic cells by DCs at various levels of maturation was assessed, 61 +/- 7% of immature DCs (iDCs) internalized fluorescence-labelled necrotic material. Apoptotic material from UV-B-irradiated cells was internalized by only 48 +/- 5% of iDCs. Maturation-inducing cytokines did not affect the uptake when added simultaneously with the tumor cell preparations. Loading DCs with heat-necrotic or apoptotic melanoma cells slightly reduced CD83 expression while leaving CD208 (DC-LAMP) expression unchanged. As determined by IFN-gamma-detecting enzyme-linked-immunospot assays, iDCs loaded with heat-killed melanoma cells activated autologous T cells most effectively when used without any further maturation, whereas DCs loaded with apoptotic material required maturation. In conclusion, HSP70-expressing melanoma cells could be generated by heat-killing. Loading iDCs with heat-killed melanoma cells resulted in a superior priming of autologous T cells in vitro." @default.
• W2071434627 created "2016-06-24" @default.
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• W2071434627 date "2010-02-01" @default.
• W2071434627 modified "2023-09-25" @default.
• W2071434627 title "Enhanced T-cell activation by immature dendritic cells loaded with HSP70-expressing heat-killed melanoma cells" @default.
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• W2071434627 doi "https://doi.org/10.1111/j.1600-0625.2009.00962.x" @default.
• W2071434627 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19758341" @default.
• W2071434627 hasPublicationYear "2010" @default.
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