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- W2071457012 abstract "A novel label-free strategy for the detection of bacteria was developed by using a specific antimicrobial peptide (AMP)-functionalized quartz crystal microbalance (QCM) electrode. This electrode interface was successfully applied to detect pathogenic Escherichia coli O157:H7 based on the specific affinity between the small synthetic antimicrobial peptide and the bacterial cell of pathogenic E. coli O157:H7. The concentrations of pathogenic E. coli O157:H7 were sensitively measured by the frequency response of the QCM with a detection limit of 0.4 cfu μL−1. The detection can be fulfilled within 10 min because it does not require germiculture process. On the other hand, if the specific antimicrobial peptides were immobilized on a gold electrode, this label-free strategy can also be performed by electrochemical impedance spectroscopy (EIS). Compared with QCM technique, the EIS measurement gives a lower sensitivity and needs a longer assay time. The combination of antimicrobial peptides with the real-time responses of QCM, as well as electronic read-out monitoring of EIS, may open a new way for the direct detection of bacteria." @default.
- W2071457012 created "2016-06-24" @default.
- W2071457012 creator A5042108591 @default.
- W2071457012 creator A5052057182 @default.
- W2071457012 date "2015-05-01" @default.
- W2071457012 modified "2023-09-27" @default.
- W2071457012 title "Label-free detection of pathogenic bacteria via immobilized antimicrobial peptides" @default.
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- W2071457012 doi "https://doi.org/10.1016/j.talanta.2015.01.015" @default.
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