Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071464513> ?p ?o ?g. }
- W2071464513 endingPage "384" @default.
- W2071464513 startingPage "361" @default.
- W2071464513 abstract "1. We have used laser micromass analysis (l.a.m.m.a.) to investigate Ca uptake and release in intact 'red' rod photoreceptors in the dark-adapted retina of the toad, Bufo marinus. 2. With l.a.m.m.a. it is possible to measure separately the concentrations of each of the Ca isotopes. Rods normally containing almost exclusively 40Ca can be incubated in Ringer solution containing the stable isotopes 42Ca or 44Ca. In this way, the movements of Ca into and out of the rod can be separately determined. 3. When rods are incubated in darkness in high 44Ca (up to 20 mM), large amounts of 44Ca accumulate in the outer segment at a rate which increases with increasing external 44Ca concentration. However, this 44Ca appears not to exchange with the 40Ca originally present within the rod. This result suggests that the 40Ca may be sequestered within a pool which normally exchanges slowly with external Ca. 4. We explored Ca exchange in high-Ca solutions in more detail with double-isotope labelling. In these experiments, rods were first pre-loaded with Ca of one isotope (42Ca) and then incubated in Ringer solution containing another (44Ca). We could then measure separately the rate of exchange of the pre-loaded 42Ca with the 44Ca in the Ringer solution and with the 40Ca originally present within the rod in the sequestered pool. 5. These experiments show that the pre-loaded-Ca exchanges rapidly with Ca in the Ringer solution, at least in part by Ca-Ca exchange, but much more slowly with the Ca originally present within the rod. Thus Ca in the outer segments can exist in (at least) two pools: one which exchanges rapidly across the plasma membrane and is probably Ca free or loosely bound within the cytosol, and another which exchanges slowly and is probably Ca within the disks. 6. Although Ca sequestered within the outer segment normally exchanges quite slowly, it can be rapidly released if the extracellular free Ca is buffered to low levels with EGTA. The rate-limiting step for Ca release under these conditions appears not to be Na-Ca exchange, since the rate of Ca efflux is unchanged if the Na in the Ringer solution is substituted with choline. 7. Ca can also be released from the sequestered pool if rods are incubated in Ringer solution containing 100 or 500 microM-IBMX (3-isobutyl-1-methylxanthine).(ABSTRACT TRUNCATED AT 400 WORDS)" @default.
- W2071464513 created "2016-06-24" @default.
- W2071464513 creator A5044248191 @default.
- W2071464513 creator A5045905817 @default.
- W2071464513 date "1987-08-01" @default.
- W2071464513 modified "2023-09-26" @default.
- W2071464513 title "Calcium in dark-adapted toad rods: evidence for pooling and cyclic-guanosine-3'-5'-monophosphate-dependent release." @default.
- W2071464513 cites W111988334 @default.
- W2071464513 cites W1563310899 @default.
- W2071464513 cites W1964253397 @default.
- W2071464513 cites W1968726533 @default.
- W2071464513 cites W1974191268 @default.
- W2071464513 cites W1978789853 @default.
- W2071464513 cites W1981151574 @default.
- W2071464513 cites W1996438246 @default.
- W2071464513 cites W2021091114 @default.
- W2071464513 cites W2022450451 @default.
- W2071464513 cites W2023104948 @default.
- W2071464513 cites W2025364863 @default.
- W2071464513 cites W2035567650 @default.
- W2071464513 cites W2037969105 @default.
- W2071464513 cites W2040047419 @default.
- W2071464513 cites W2045546014 @default.
- W2071464513 cites W2065540757 @default.
- W2071464513 cites W2066453053 @default.
- W2071464513 cites W2072569332 @default.
- W2071464513 cites W2074563689 @default.
- W2071464513 cites W2075769143 @default.
- W2071464513 cites W2075855004 @default.
- W2071464513 cites W2080454286 @default.
- W2071464513 cites W2082873326 @default.
- W2071464513 cites W2085855700 @default.
- W2071464513 cites W2089386102 @default.
- W2071464513 cites W2099030341 @default.
- W2071464513 cites W2106781808 @default.
- W2071464513 cites W2123874680 @default.
- W2071464513 cites W2132194645 @default.
- W2071464513 cites W2144993030 @default.
- W2071464513 cites W2152821561 @default.
- W2071464513 cites W2177086883 @default.
- W2071464513 cites W2396674895 @default.
- W2071464513 doi "https://doi.org/10.1113/jphysiol.1987.sp016661" @default.
- W2071464513 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1192085" @default.
- W2071464513 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2445976" @default.
- W2071464513 hasPublicationYear "1987" @default.
- W2071464513 type Work @default.
- W2071464513 sameAs 2071464513 @default.
- W2071464513 citedByCount "25" @default.
- W2071464513 crossrefType "journal-article" @default.
- W2071464513 hasAuthorship W2071464513A5044248191 @default.
- W2071464513 hasAuthorship W2071464513A5045905817 @default.
- W2071464513 hasBestOaLocation W20714645131 @default.
- W2071464513 hasConcept C121332964 @default.
- W2071464513 hasConcept C12554922 @default.
- W2071464513 hasConcept C134018914 @default.
- W2071464513 hasConcept C142724271 @default.
- W2071464513 hasConcept C162184086 @default.
- W2071464513 hasConcept C164304813 @default.
- W2071464513 hasConcept C178790620 @default.
- W2071464513 hasConcept C185592680 @default.
- W2071464513 hasConcept C189701845 @default.
- W2071464513 hasConcept C204787440 @default.
- W2071464513 hasConcept C2778166480 @default.
- W2071464513 hasConcept C2779027399 @default.
- W2071464513 hasConcept C2909474611 @default.
- W2071464513 hasConcept C519063684 @default.
- W2071464513 hasConcept C59822182 @default.
- W2071464513 hasConcept C62520636 @default.
- W2071464513 hasConcept C71924100 @default.
- W2071464513 hasConcept C86803240 @default.
- W2071464513 hasConceptScore W2071464513C121332964 @default.
- W2071464513 hasConceptScore W2071464513C12554922 @default.
- W2071464513 hasConceptScore W2071464513C134018914 @default.
- W2071464513 hasConceptScore W2071464513C142724271 @default.
- W2071464513 hasConceptScore W2071464513C162184086 @default.
- W2071464513 hasConceptScore W2071464513C164304813 @default.
- W2071464513 hasConceptScore W2071464513C178790620 @default.
- W2071464513 hasConceptScore W2071464513C185592680 @default.
- W2071464513 hasConceptScore W2071464513C189701845 @default.
- W2071464513 hasConceptScore W2071464513C204787440 @default.
- W2071464513 hasConceptScore W2071464513C2778166480 @default.
- W2071464513 hasConceptScore W2071464513C2779027399 @default.
- W2071464513 hasConceptScore W2071464513C2909474611 @default.
- W2071464513 hasConceptScore W2071464513C519063684 @default.
- W2071464513 hasConceptScore W2071464513C59822182 @default.
- W2071464513 hasConceptScore W2071464513C62520636 @default.
- W2071464513 hasConceptScore W2071464513C71924100 @default.
- W2071464513 hasConceptScore W2071464513C86803240 @default.
- W2071464513 hasIssue "1" @default.
- W2071464513 hasLocation W20714645131 @default.
- W2071464513 hasLocation W20714645132 @default.
- W2071464513 hasLocation W20714645133 @default.
- W2071464513 hasOpenAccess W2071464513 @default.
- W2071464513 hasPrimaryLocation W20714645131 @default.
- W2071464513 hasRelatedWork W1981783083 @default.
- W2071464513 hasRelatedWork W1982546453 @default.
- W2071464513 hasRelatedWork W2015073828 @default.
- W2071464513 hasRelatedWork W2060571654 @default.