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- W2071496543 abstract "Synaptophysin (SY) is an integral membrane protein of presynaptic small (30–80-nm) translucent vesicles also present in dispersed neuroendocrine cells. As the occurrence of this type of vesicle is specific for two major pathways of differentiation, the neuronal and neuroendocrine-epithelial information on the regulation of SY synthesis should contribute to an understanding of regulatory principles common to both pathways. Isolation and comparison of the complete rat and human single-copy genes showed that despite the difference in size (16kb in rat vs. 13 kb in man) intron/exon boundaries are precisely conserved. Surprisingly, intron VI is located in the 3'-noncoding region in both species. The major transcriptional start point, as determined by primer extension and S1-nuclease protection analyses in rat pheochromocytoma-derived PC 12 cells and rat brain, mapped to a site 27 nt 5' of the first methionine codon. Unexpectedly, the 5' upstream region is devoid of any TATA or CAAT boxes, but shows instead typical features of ‘housekeeping’ genes i.e., G + C-rich islands and four Spl-binding motifs. Using ‘nuclear run-on’ assays, we have identified examples in which SY synthesis is regulated at the transcriptional level. Reporter gene constructs showed that approx. 1.2kb of the immediate upstream region contains promoter/enhancer elements that were, however, insufficient to confer cell-type specific expression, whereas sequences farther upstream were able to suppress thymidine kinase promoter activity in a cell-type-dependent fashion." @default.
- W2071496543 created "2016-06-24" @default.
- W2071496543 creator A5029898558 @default.
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- W2071496543 date "1991-03-01" @default.
- W2071496543 modified "2023-10-16" @default.
- W2071496543 title "The synaptophysin-encoding gene in rat and man is specifically transcribed in neuroendocrine cells" @default.
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- W2071496543 doi "https://doi.org/10.1016/0378-1119(91)90127-w" @default.
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