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• W2071524230 endingPage "136" @default.
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• W2071524230 abstract "Nasopharyngeal carcinoma (NPC) is one of the high population malignant tumors among Chinese in southern China and southeast Asia. Epstein–Barr virus (EBV) is a human B lymphotropic herpes virus which is known to be closely associated with NPC. EBV DNA polymerase is a key enzyme during EBV replication and is measured by its radioactivity. The addition of phorbol 12-myristate 13-acetate to Raji cell cultures led to a large increase in EBV DNA polymerase, which was purified by sequential DEAE–cellulose, phosphocellulose and DNA–cellulose column chromatography. Four tannins were isolated from the active fractions of Eugenia uniflora L., which were tested for the inhibition of EBV DNA polymerase. The results showed the 50% inhibitory concentration (IC50) values of gallocatechin, oenothein B, eugeniflorins D1 and D2 were 26.5 62.3, 3.0 and 3.5 μM, respectively. Furthermore, when compared with the positive control (phosphonoacetic acid), an inhibitor of EBV replication, the IC50 value was 16.4 μM. In view of the results, eugeniflorins D1 and D2 are the potency principles in the inhibition of EBV DNA polymerase from E. uniflora." @default.
• W2071524230 created "2016-06-24" @default.
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• W2071524230 date "2000-06-01" @default.
• W2071524230 modified "2023-09-27" @default.
• W2071524230 title "EBV DNA polymerase inhibition of tannins from Eugenia uniflora" @default.
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• W2071524230 doi "https://doi.org/10.1016/s0304-3835(00)00353-0" @default.
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