FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071530132> ?p ?o ?g. }

• W2071530132 endingPage "165" @default.
• W2071530132 startingPage "159" @default.
• W2071530132 abstract "Membranes with suitable mechanical properties, chemical stability, and macroporosity which have a selectivity for trypsin were prepared by coupling a ligand (p-aminobenzamidine) to cross-linked chitosan membranes via a spacer (succinic acid). The chitosan membranes were cross-linked with ethylene glycol diglycidyl ether in order to avoid their dissolution in acidic media. The cross-linked chitosan membranes were then covalently coupled with succinic anhydride, at ambient temperature, to obtain hydrophilic spacers with carboxyl terminals. The amino groups of the chitosan membranes which have not reacted with the succinic anhydride were blocked with acetic anhydride to avoid the nonspecific interactions. Finally the ligand p-aminobenzamidine (PAB) was incorporated via the coupling between the carboxyl groups of the succinic acid and the amino groups of PAB, using 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide (EDAC) as the activating agent. A PAB density of 2.7 × 10-3 mol/g of chitosan was achieved. Since the PAB−chitosan membranes have a much higher affinity toward trypsin than toward chymotrypsin, they have been employed for the purification of crude trypsin solutions. A purification factor > 7, a specific activity > 9500 BAEE units/mg, and activity yields between 60 and 80% were obtained. These results suggest that the PAB−chitosan affinity membrane can be efficient for trypsin purification." @default.
• W2071530132 created "2016-06-24" @default.
• W2071530132 creator A5033543687 @default.
• W2071530132 creator A5037903674 @default.
• W2071530132 date "1998-01-01" @default.
• W2071530132 modified "2023-10-07" @default.
• W2071530132 title "Trypsin Purification by <i>p</i>-Aminobenzamidine Immobilized on Macroporous Chitosan Membranes" @default.
• W2071530132 cites W1805851852 @default.
• W2071530132 cites W1972295378 @default.
• W2071530132 cites W1975933303 @default.
• W2071530132 cites W1978844591 @default.
• W2071530132 cites W1980663466 @default.
• W2071530132 cites W1981756316 @default.
• W2071530132 cites W1994822835 @default.
• W2071530132 cites W1996852601 @default.
• W2071530132 cites W2014930031 @default.
• W2071530132 cites W2021331272 @default.
• W2071530132 cites W2025331052 @default.
• W2071530132 cites W2043101031 @default.
• W2071530132 cites W2051487106 @default.
• W2071530132 cites W2066931414 @default.
• W2071530132 cites W2084034925 @default.
• W2071530132 cites W2086409740 @default.
• W2071530132 cites W2086982477 @default.
• W2071530132 cites W2089416853 @default.
• W2071530132 cites W2091642154 @default.
• W2071530132 cites W2104220801 @default.
• W2071530132 cites W2107186186 @default.
• W2071530132 cites W2904214728 @default.
• W2071530132 cites W4293247451 @default.
• W2071530132 doi "https://doi.org/10.1021/ie970466z" @default.
• W2071530132 hasPublicationYear "1998" @default.
• W2071530132 type Work @default.
• W2071530132 sameAs 2071530132 @default.
• W2071530132 citedByCount "34" @default.
• W2071530132 countsByYear W20715301322012 @default.
• W2071530132 countsByYear W20715301322013 @default.
• W2071530132 countsByYear W20715301322016 @default.
• W2071530132 countsByYear W20715301322017 @default.
• W2071530132 countsByYear W20715301322019 @default.
• W2071530132 countsByYear W20715301322021 @default.
• W2071530132 crossrefType "journal-article" @default.
• W2071530132 hasAuthorship W2071530132A5033543687 @default.
• W2071530132 hasAuthorship W2071530132A5037903674 @default.
• W2071530132 hasConcept C10138342 @default.
• W2071530132 hasConcept C162324750 @default.
• W2071530132 hasConcept C166595027 @default.
• W2071530132 hasConcept C178790620 @default.
• W2071530132 hasConcept C180577832 @default.
• W2071530132 hasConcept C181199279 @default.
• W2071530132 hasConcept C184133267 @default.
• W2071530132 hasConcept C185592680 @default.
• W2071530132 hasConcept C188027245 @default.
• W2071530132 hasConcept C2775997276 @default.
• W2071530132 hasConcept C2776436638 @default.
• W2071530132 hasConcept C2777516009 @default.
• W2071530132 hasConcept C2777735259 @default.
• W2071530132 hasConcept C2779684741 @default.
• W2071530132 hasConcept C2779732960 @default.
• W2071530132 hasConcept C2780340462 @default.
• W2071530132 hasConcept C2781041046 @default.
• W2071530132 hasConcept C41625074 @default.
• W2071530132 hasConcept C43617362 @default.
• W2071530132 hasConcept C54400483 @default.
• W2071530132 hasConcept C55493867 @default.
• W2071530132 hasConceptScore W2071530132C10138342 @default.
• W2071530132 hasConceptScore W2071530132C162324750 @default.
• W2071530132 hasConceptScore W2071530132C166595027 @default.
• W2071530132 hasConceptScore W2071530132C178790620 @default.
• W2071530132 hasConceptScore W2071530132C180577832 @default.
• W2071530132 hasConceptScore W2071530132C181199279 @default.
• W2071530132 hasConceptScore W2071530132C184133267 @default.
• W2071530132 hasConceptScore W2071530132C185592680 @default.
• W2071530132 hasConceptScore W2071530132C188027245 @default.
• W2071530132 hasConceptScore W2071530132C2775997276 @default.
• W2071530132 hasConceptScore W2071530132C2776436638 @default.
• W2071530132 hasConceptScore W2071530132C2777516009 @default.
• W2071530132 hasConceptScore W2071530132C2777735259 @default.
• W2071530132 hasConceptScore W2071530132C2779684741 @default.
• W2071530132 hasConceptScore W2071530132C2779732960 @default.
• W2071530132 hasConceptScore W2071530132C2780340462 @default.
• W2071530132 hasConceptScore W2071530132C2781041046 @default.
• W2071530132 hasConceptScore W2071530132C41625074 @default.
• W2071530132 hasConceptScore W2071530132C43617362 @default.
• W2071530132 hasConceptScore W2071530132C54400483 @default.
• W2071530132 hasConceptScore W2071530132C55493867 @default.
• W2071530132 hasIssue "1" @default.
• W2071530132 hasLocation W20715301321 @default.
• W2071530132 hasOpenAccess W2071530132 @default.
• W2071530132 hasPrimaryLocation W20715301321 @default.
• W2071530132 hasRelatedWork W1970248427 @default.
• W2071530132 hasRelatedWork W2027634528 @default.
• W2071530132 hasRelatedWork W2076809730 @default.
• W2071530132 hasRelatedWork W2102511969 @default.
• W2071530132 hasRelatedWork W2498456940 @default.
• W2071530132 hasRelatedWork W2961251311 @default.
• W2071530132 hasRelatedWork W2992251384 @default.
• W2071530132 hasRelatedWork W3166507656 @default.

• next