Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071566197> ?p ?o ?g. }
- W2071566197 endingPage "24" @default.
- W2071566197 startingPage "14" @default.
- W2071566197 abstract "The effect of thioredoxin peroxidases on the protection of Ca2+-induced inner mitochondrial membrane permeabilization was studied in the yeast Saccharomyces cerevisiae using null mutants for these genes. Since deletion of a gene can promote several other effects besides the absence of the respective protein, characterizations of the redox state of the mutant strains were performed. Whole cellular extracts from all the mutants presented lower capacity to decompose H2O2 and lower GSH/GSSG ratios, as expected for strains deficient for peroxide-removing enzymes. Interestingly, when glutathione contents in mitochondrial pools were analyzed, all mutants presented lower GSH/GSSG ratios than wild-type cells, with the exception of ΔcTPxI strain (cells in which cytosolic thioredoxin peroxidase I gene was disrupted) that presented higher GSH/GSSG ratio. Low GSH/GSSG ratios in mitochondria increased the susceptibility of yeast to damage induced by Ca2+ as determined by membrane potential and oxygen consumption experiments. However, H2O2 removal activity appears also to be important for mitochondria protection against permeabilization because exogenously added catalase strongly inhibited loss of mitochondrial potential. Moreover, exogenously added recombinant peroxiredoxins prevented inner mitochondrial membrane permeabilization. GSH/GSSG ratios decreased after Ca2+ addition, suggesting that reactive oxygen species (ROS) probably mediate this process. Taken together our results indicate that both mitochondrial glutathione pools and peroxide-removing enzymes are key components for the protection of yeast mitochondria against Ca2+-induced damage." @default.
- W2071566197 created "2016-06-24" @default.
- W2071566197 creator A5011740095 @default.
- W2071566197 creator A5049076742 @default.
- W2071566197 creator A5051359796 @default.
- W2071566197 creator A5075100345 @default.
- W2071566197 date "2004-05-01" @default.
- W2071566197 modified "2023-09-29" @default.
- W2071566197 title "Glutathione and thioredoxin peroxidases mediate susceptibility of yeast mitochondria to Ca2+-induced damage" @default.
- W2071566197 cites W1270671049 @default.
- W2071566197 cites W1487157100 @default.
- W2071566197 cites W1546256108 @default.
- W2071566197 cites W1547929361 @default.
- W2071566197 cites W1550007121 @default.
- W2071566197 cites W1763161027 @default.
- W2071566197 cites W1845564569 @default.
- W2071566197 cites W1963778474 @default.
- W2071566197 cites W1970768581 @default.
- W2071566197 cites W1970881583 @default.
- W2071566197 cites W1978226123 @default.
- W2071566197 cites W1978240379 @default.
- W2071566197 cites W1988966589 @default.
- W2071566197 cites W1989284933 @default.
- W2071566197 cites W1991533782 @default.
- W2071566197 cites W1993055408 @default.
- W2071566197 cites W1997313112 @default.
- W2071566197 cites W1999392732 @default.
- W2071566197 cites W1999748325 @default.
- W2071566197 cites W2002204441 @default.
- W2071566197 cites W2002823937 @default.
- W2071566197 cites W2008608484 @default.
- W2071566197 cites W2013557200 @default.
- W2071566197 cites W2016862957 @default.
- W2071566197 cites W2018088135 @default.
- W2071566197 cites W2036302841 @default.
- W2071566197 cites W2038017519 @default.
- W2071566197 cites W2045753882 @default.
- W2071566197 cites W2046910842 @default.
- W2071566197 cites W2053002124 @default.
- W2071566197 cites W2056102666 @default.
- W2071566197 cites W2057818847 @default.
- W2071566197 cites W2063385957 @default.
- W2071566197 cites W2072623280 @default.
- W2071566197 cites W2086138681 @default.
- W2071566197 cites W2086445948 @default.
- W2071566197 cites W2087598382 @default.
- W2071566197 cites W2087811721 @default.
- W2071566197 cites W2089062179 @default.
- W2071566197 cites W2094844253 @default.
- W2071566197 cites W2098644206 @default.
- W2071566197 cites W2112696881 @default.
- W2071566197 cites W2113999599 @default.
- W2071566197 cites W2141949196 @default.
- W2071566197 cites W2148033110 @default.
- W2071566197 cites W2166788705 @default.
- W2071566197 cites W2167450976 @default.
- W2071566197 cites W2169805130 @default.
- W2071566197 cites W2169875902 @default.
- W2071566197 cites W238201244 @default.
- W2071566197 doi "https://doi.org/10.1016/j.abb.2004.03.005" @default.
- W2071566197 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15081889" @default.
- W2071566197 hasPublicationYear "2004" @default.
- W2071566197 type Work @default.
- W2071566197 sameAs 2071566197 @default.
- W2071566197 citedByCount "33" @default.
- W2071566197 countsByYear W20715661972013 @default.
- W2071566197 countsByYear W20715661972014 @default.
- W2071566197 countsByYear W20715661972015 @default.
- W2071566197 countsByYear W20715661972017 @default.
- W2071566197 countsByYear W20715661972019 @default.
- W2071566197 countsByYear W20715661972021 @default.
- W2071566197 countsByYear W20715661972023 @default.
- W2071566197 crossrefType "journal-article" @default.
- W2071566197 hasAuthorship W2071566197A5011740095 @default.
- W2071566197 hasAuthorship W2071566197A5049076742 @default.
- W2071566197 hasAuthorship W2071566197A5051359796 @default.
- W2071566197 hasAuthorship W2071566197A5075100345 @default.
- W2071566197 hasConcept C104317684 @default.
- W2071566197 hasConcept C143065580 @default.
- W2071566197 hasConcept C162008176 @default.
- W2071566197 hasConcept C181199279 @default.
- W2071566197 hasConcept C185592680 @default.
- W2071566197 hasConcept C2775838275 @default.
- W2071566197 hasConcept C2776151105 @default.
- W2071566197 hasConcept C2777576037 @default.
- W2071566197 hasConcept C2778175917 @default.
- W2071566197 hasConcept C2778979269 @default.
- W2071566197 hasConcept C2779222958 @default.
- W2071566197 hasConcept C28859421 @default.
- W2071566197 hasConcept C3623737 @default.
- W2071566197 hasConcept C48349386 @default.
- W2071566197 hasConcept C538909803 @default.
- W2071566197 hasConcept C55493867 @default.
- W2071566197 hasConcept C75385678 @default.
- W2071566197 hasConcept C86803240 @default.
- W2071566197 hasConcept C98539663 @default.
- W2071566197 hasConceptScore W2071566197C104317684 @default.
- W2071566197 hasConceptScore W2071566197C143065580 @default.