FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071572040> ?p ?o ?g. }

• W2071572040 endingPage "8999" @default.
• W2071572040 startingPage "8992" @default.
• W2071572040 abstract "The nickel−iron hydrogenase from Chromatium vinosum adsorbs at a pyrolytic graphite edge-plane (PGE) electrode and catalyzes rapid interconversion of H+(aq) and H2 at potentials expected for the half-cell reaction 2H+ ⇄ H2, i.e., without the need for overpotentials. The voltammetry mirrors characteristics determined by conventional methods, while affording the capabilities for exquisite control and measurement of potential-dependent activities and substrate−product mass transport. Oxidation of H2 is extremely rapid; at 10% partial pressure H2, mass transport control persists even at the highest electrode rotation rates. The turnover number for H2 oxidation lies in the range of 1500−9000 s-1 at 30 °C (pH 5−8), which is significantly higher than that observed using methylene blue as the electron acceptor. By contrast, proton reduction is slower and controlled by processes occurring in the enzyme. Carbon monoxide, which binds reversibly to the NiFe site in the active form, inhibits electrocatalysis and allows improved definition of signals that can be attributed to the reversible (non-turnover) oxidation and reduction of redox centers. One signal, at −30 mV vs SHE (pH 7.0, 30 °C), is assigned to the [3Fe-4S]+/0 cluster on the basis of potentiometric measurements. The second, at −301 mV and having a 1.5−2.5-fold greater amplitude, is tentatively assigned to the two [4Fe-4S]2+/+ clusters with similar reduction potentials. No other redox couples are observed, suggesting that these two sets of centers are the only ones in CO-inhibited hydrogenase capable of undergoing simple rapid cycling of their redox states. With the buried NiFe active site very unlikely to undergo direct electron exchange with the electrode, at least one and more likely each of the three iron−sulfur clusters must serve as relay sites. The fact that H2 oxidation is rapid even at potentials nearly 300 mV more negative than the reduction potential of the [3Fe-4S]+/0 cluster shows that its singularly high equilibrium reduction potential does not compromise catalytic efficiency." @default.
• W2071572040 created "2016-06-24" @default.
• W2071572040 creator A5010638664 @default.
• W2071572040 creator A5017462847 @default.
• W2071572040 creator A5044637051 @default.
• W2071572040 creator A5078374648 @default.
• W2071572040 creator A5081123043 @default.
• W2071572040 creator A5085638660 @default.
• W2071572040 date "1999-06-23" @default.
• W2071572040 modified "2023-10-16" @default.
• W2071572040 title "Catalytic Electron Transport in <i>Chromatium vinosum</i> [NiFe]-Hydrogenase: Application of Voltammetry in Detecting Redox-Active Centers and Establishing That Hydrogen Oxidation Is Very Fast Even at Potentials Close to the Reversible H⁺/H₂ Value" @default.
• W2071572040 cites W1551671769 @default.
• W2071572040 cites W1630260213 @default.
• W2071572040 cites W1981106248 @default.
• W2071572040 cites W1997005501 @default.
• W2071572040 cites W1997053191 @default.
• W2071572040 cites W2007856369 @default.
• W2071572040 cites W2011587949 @default.
• W2071572040 cites W2033583737 @default.
• W2071572040 cites W2040499879 @default.
• W2071572040 cites W2059081508 @default.
• W2071572040 cites W2065264552 @default.
• W2071572040 cites W2087875520 @default.
• W2071572040 cites W2092067755 @default.
• W2071572040 cites W2118492712 @default.
• W2071572040 cites W2150267644 @default.
• W2071572040 cites W2171071880 @default.
• W2071572040 cites W2178280253 @default.
• W2071572040 doi "https://doi.org/10.1021/bi990108v" @default.
• W2071572040 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10413472" @default.
• W2071572040 hasPublicationYear "1999" @default.
• W2071572040 type Work @default.
• W2071572040 sameAs 2071572040 @default.
• W2071572040 citedByCount "226" @default.
• W2071572040 countsByYear W20715720402012 @default.
• W2071572040 countsByYear W20715720402013 @default.
• W2071572040 countsByYear W20715720402014 @default.
• W2071572040 countsByYear W20715720402015 @default.
• W2071572040 countsByYear W20715720402016 @default.
• W2071572040 countsByYear W20715720402017 @default.
• W2071572040 countsByYear W20715720402018 @default.
• W2071572040 countsByYear W20715720402019 @default.
• W2071572040 countsByYear W20715720402020 @default.
• W2071572040 countsByYear W20715720402021 @default.
• W2071572040 countsByYear W20715720402022 @default.
• W2071572040 countsByYear W20715720402023 @default.
• W2071572040 crossrefType "journal-article" @default.
• W2071572040 hasAuthorship W2071572040A5010638664 @default.
• W2071572040 hasAuthorship W2071572040A5017462847 @default.
• W2071572040 hasAuthorship W2071572040A5044637051 @default.
• W2071572040 hasAuthorship W2071572040A5078374648 @default.
• W2071572040 hasAuthorship W2071572040A5081123043 @default.
• W2071572040 hasAuthorship W2071572040A5085638660 @default.
• W2071572040 hasConcept C129696212 @default.
• W2071572040 hasConcept C147789679 @default.
• W2071572040 hasConcept C161790260 @default.
• W2071572040 hasConcept C17525397 @default.
• W2071572040 hasConcept C178790620 @default.
• W2071572040 hasConcept C179104552 @default.
• W2071572040 hasConcept C185592680 @default.
• W2071572040 hasConcept C24840226 @default.
• W2071572040 hasConcept C2777107655 @default.
• W2071572040 hasConcept C2777869211 @default.
• W2071572040 hasConcept C2778343803 @default.
• W2071572040 hasConcept C33989665 @default.
• W2071572040 hasConcept C52859227 @default.
• W2071572040 hasConcept C55493867 @default.
• W2071572040 hasConcept C55904794 @default.
• W2071572040 hasConcept C75473681 @default.
• W2071572040 hasConcept C79115784 @default.
• W2071572040 hasConceptScore W2071572040C129696212 @default.
• W2071572040 hasConceptScore W2071572040C147789679 @default.
• W2071572040 hasConceptScore W2071572040C161790260 @default.
• W2071572040 hasConceptScore W2071572040C17525397 @default.
• W2071572040 hasConceptScore W2071572040C178790620 @default.
• W2071572040 hasConceptScore W2071572040C179104552 @default.
• W2071572040 hasConceptScore W2071572040C185592680 @default.
• W2071572040 hasConceptScore W2071572040C24840226 @default.
• W2071572040 hasConceptScore W2071572040C2777107655 @default.
• W2071572040 hasConceptScore W2071572040C2777869211 @default.
• W2071572040 hasConceptScore W2071572040C2778343803 @default.
• W2071572040 hasConceptScore W2071572040C33989665 @default.
• W2071572040 hasConceptScore W2071572040C52859227 @default.
• W2071572040 hasConceptScore W2071572040C55493867 @default.
• W2071572040 hasConceptScore W2071572040C55904794 @default.
• W2071572040 hasConceptScore W2071572040C75473681 @default.
• W2071572040 hasConceptScore W2071572040C79115784 @default.
• W2071572040 hasIssue "28" @default.
• W2071572040 hasLocation W20715720401 @default.
• W2071572040 hasLocation W20715720402 @default.
• W2071572040 hasOpenAccess W2071572040 @default.
• W2071572040 hasPrimaryLocation W20715720401 @default.
• W2071572040 hasRelatedWork W1502149024 @default.
• W2071572040 hasRelatedWork W1978267032 @default.
• W2071572040 hasRelatedWork W1999593514 @default.
• W2071572040 hasRelatedWork W2012207277 @default.
• W2071572040 hasRelatedWork W2022631658 @default.
• W2071572040 hasRelatedWork W2043704213 @default.

• next