FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071610288> ?p ?o ?g. }

• W2071610288 endingPage "206" @default.
• W2071610288 startingPage "199" @default.
• W2071610288 abstract "The ATP-dependent transport of natural product drugs, e.g. vincristine, by multidrug resistance-associated protein (MRP1) requires reduced glutathione (GSH), whilst that of anionic substrates does not. The present results suggest, however, that GSH can modulate transport of anionic species. Efflux of fluorescent anionic substrates was measured from adherent MRP1-expressing human multidrug-resistant lung tumour cells, COR-L23/R, and drug-sensitive parental cells. As expected, much greater efflux of calcein, methylfluorescein-glutathione (GS-MF), and 2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) was observed from the resistant cells. Unexpectedly, lowering GSH levels in COR-L23/R cells by inhibiting GSH synthesis with buthionine sulfoximine decreased efflux of calcein and of GS-MF (3-fold and 1.6-fold) but not efflux of BCECF. Transport of the anionic conjugate dinitrophenyl-glutathione ([3H]DNP-SG) was investigated by following its uptake into inside-out plasma membrane vesicles prepared from the MRP1-expressing cells. At least 90% of the ATP-dependent uptake was blockable by the anti-MRP1 antibody QCRL-3 and 100 μM vincristine inhibited uptake but only in the presence of 1–3 mM GSH, suggesting MRP1 to be the protein primarily responsible for this transport. Agents shown to reduce efflux of calcein from resistant cells, i.e. indomethacin, MK-571, and probenecid, also inhibited [3H]DNP-SG uptakes, consistent with MRP1 being responsible for export of calcein. At concentrations achievable within cells, GSSG (70 μM) inhibited uptake whereas GSH (1 and 3 mM) enhanced uptake. We suggest that variations in both GSH and GSSG levels within cells may affect MRP1-mediated anion transport." @default.
• W2071610288 created "2016-06-24" @default.
• W2071610288 creator A5027013809 @default.
• W2071610288 creator A5030642912 @default.
• W2071610288 creator A5032037823 @default.
• W2071610288 creator A5054429563 @default.
• W2071610288 date "2001-07-01" @default.
• W2071610288 modified "2023-09-27" @default.
• W2071610288 title "Influences of glutathione on anionic substrate efflux in tumour cells expressing the multidrug resistance-associated protein, MRP111Abbreviations: BCECF, 2′,7′-bis-(2-carboxyethel)-5-(and-6)-carboxyfluorescein; BCECF-AM, 2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester; BSO, buthionine sulfoximine; calcein-AM, calcein acetoxymethyl ester; CMFDA, chloromethylfluorescein diacetate; DNP-SG, dinitrophenyl-S-glutathione; GS-MF, methylfluorescein-glutathione; MRP1, …" @default.
• W2071610288 cites W1502426706 @default.
• W2071610288 cites W1567645277 @default.
• W2071610288 cites W1965129145 @default.
• W2071610288 cites W1989517413 @default.
• W2071610288 cites W2000980801 @default.
• W2071610288 cites W2004804291 @default.
• W2071610288 cites W2012841076 @default.
• W2071610288 cites W2014051119 @default.
• W2071610288 cites W2028125003 @default.
• W2071610288 cites W2040906015 @default.
• W2071610288 cites W2046262741 @default.
• W2071610288 cites W2063576996 @default.
• W2071610288 cites W2065158529 @default.
• W2071610288 cites W2076623448 @default.
• W2071610288 cites W2080736726 @default.
• W2071610288 cites W2085452141 @default.
• W2071610288 cites W2092067397 @default.
• W2071610288 cites W2114195555 @default.
• W2071610288 cites W2147566151 @default.
• W2071610288 cites W2149994120 @default.
• W2071610288 cites W2150765758 @default.
• W2071610288 cites W2168095256 @default.
• W2071610288 cites W2290695793 @default.
• W2071610288 cites W2312791182 @default.
• W2071610288 cites W77471785 @default.
• W2071610288 doi "https://doi.org/10.1016/s0006-2952(01)00660-8" @default.
• W2071610288 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11389878" @default.
• W2071610288 hasPublicationYear "2001" @default.
• W2071610288 type Work @default.
• W2071610288 sameAs 2071610288 @default.
• W2071610288 citedByCount "37" @default.
• W2071610288 countsByYear W20716102882013 @default.
• W2071610288 countsByYear W20716102882014 @default.
• W2071610288 countsByYear W20716102882015 @default.
• W2071610288 countsByYear W20716102882017 @default.
• W2071610288 countsByYear W20716102882020 @default.
• W2071610288 countsByYear W20716102882022 @default.
• W2071610288 countsByYear W20716102882023 @default.
• W2071610288 crossrefType "journal-article" @default.
• W2071610288 hasAuthorship W2071610288A5027013809 @default.
• W2071610288 hasAuthorship W2071610288A5030642912 @default.
• W2071610288 hasAuthorship W2071610288A5032037823 @default.
• W2071610288 hasAuthorship W2071610288A5054429563 @default.
• W2071610288 hasConcept C104317684 @default.
• W2071610288 hasConcept C12554922 @default.
• W2071610288 hasConcept C149011108 @default.
• W2071610288 hasConcept C181199279 @default.
• W2071610288 hasConcept C185592680 @default.
• W2071610288 hasConcept C200082930 @default.
• W2071610288 hasConcept C2776872141 @default.
• W2071610288 hasConcept C2777476445 @default.
• W2071610288 hasConcept C41625074 @default.
• W2071610288 hasConcept C44312359 @default.
• W2071610288 hasConcept C538909803 @default.
• W2071610288 hasConcept C55493867 @default.
• W2071610288 hasConcept C57947595 @default.
• W2071610288 hasConcept C63443662 @default.
• W2071610288 hasConcept C86803240 @default.
• W2071610288 hasConceptScore W2071610288C104317684 @default.
• W2071610288 hasConceptScore W2071610288C12554922 @default.
• W2071610288 hasConceptScore W2071610288C149011108 @default.
• W2071610288 hasConceptScore W2071610288C181199279 @default.
• W2071610288 hasConceptScore W2071610288C185592680 @default.
• W2071610288 hasConceptScore W2071610288C200082930 @default.
• W2071610288 hasConceptScore W2071610288C2776872141 @default.
• W2071610288 hasConceptScore W2071610288C2777476445 @default.
• W2071610288 hasConceptScore W2071610288C41625074 @default.
• W2071610288 hasConceptScore W2071610288C44312359 @default.
• W2071610288 hasConceptScore W2071610288C538909803 @default.
• W2071610288 hasConceptScore W2071610288C55493867 @default.
• W2071610288 hasConceptScore W2071610288C57947595 @default.
• W2071610288 hasConceptScore W2071610288C63443662 @default.
• W2071610288 hasConceptScore W2071610288C86803240 @default.
• W2071610288 hasIssue "2" @default.
• W2071610288 hasLocation W20716102881 @default.
• W2071610288 hasLocation W20716102882 @default.
• W2071610288 hasOpenAccess W2071610288 @default.
• W2071610288 hasPrimaryLocation W20716102881 @default.
• W2071610288 hasRelatedWork W153838543 @default.
• W2071610288 hasRelatedWork W1973396928 @default.
• W2071610288 hasRelatedWork W1977495485 @default.
• W2071610288 hasRelatedWork W2013628781 @default.
• W2071610288 hasRelatedWork W2033429541 @default.
• W2071610288 hasRelatedWork W2054928659 @default.
• W2071610288 hasRelatedWork W2071610288 @default.
• W2071610288 hasRelatedWork W2149994120 @default.
• W2071610288 hasRelatedWork W2162380395 @default.
• W2071610288 hasRelatedWork W2287595641 @default.
• W2071610288 hasVolume "62" @default.

• next