FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071630659> ?p ?o ?g. }

• W2071630659 endingPage "986" @default.
• W2071630659 startingPage "978" @default.
• W2071630659 abstract "Abstract The two homologous proteins ribonuclease A and onconase fold through conserved initial contacts but differ significantly in their thermodynamic stability. A disulfide bond is located in the folding initiation site of onconase (the C‐terminal part of the protein molecule) that is missing in ribonuclease A, whereas the other three disulfide bonds of onconase are conserved in ribonuclease A. Consequently, the deletion of this C‐terminal disulfide bond (C87–C104) allows the impact of the contacts in this region on the folding of onconase to be studied. We found the C87A/C104A‐onconase variant to be less active and less stable than the wild‐type protein, whereas the tertiary structure, which was determined by both X‐ray crystallography and NMR spectroscopy, was only marginally affected. The folding kinetics of the variant, however, were found to be changed considerably in comparison to wild‐type onconase. Proton exchange experiments in combination with two‐dimensional NMR spectroscopy revealed differences in the native‐state dynamics of the two proteins in the folding initiation site, which are held responsible for the changed folding mechanism. Likewise, the molecular dynamics simulation of the unfolding reaction indicated disparities for both proteins. Our results show that the high stability of onconase is based on the efficient stabilization of the folding initiation site by the C‐terminal disulfide bond. The formation of the on‐pathway intermediate, which is detectable during the folding of the wild‐type protein and promotes the fast and efficient refolding reaction, requires the presence of this covalent bond." @default.
• W2071630659 created "2016-06-24" @default.
• W2071630659 creator A5008646033 @default.
• W2071630659 creator A5026624370 @default.
• W2071630659 creator A5027275474 @default.
• W2071630659 creator A5029954648 @default.
• W2071630659 creator A5030019260 @default.
• W2071630659 creator A5041481556 @default.
• W2071630659 creator A5065393453 @default.
• W2071630659 creator A5073272119 @default.
• W2071630659 creator A5074351885 @default.
• W2071630659 date "2010-04-27" @default.
• W2071630659 modified "2023-10-13" @default.
• W2071630659 title "Impact of the C-terminal Disulfide Bond on the Folding and Stability of Onconase" @default.
• W2071630659 cites W1562498268 @default.
• W2071630659 cites W1583149693 @default.
• W2071630659 cites W1589439832 @default.
• W2071630659 cites W1593987163 @default.
• W2071630659 cites W1965826247 @default.
• W2071630659 cites W1969310616 @default.
• W2071630659 cites W1976161355 @default.
• W2071630659 cites W1984772318 @default.
• W2071630659 cites W1986191025 @default.
• W2071630659 cites W1990581038 @default.
• W2071630659 cites W1991135485 @default.
• W2071630659 cites W1994286350 @default.
• W2071630659 cites W1994354095 @default.
• W2071630659 cites W1995017064 @default.
• W2071630659 cites W1999360732 @default.
• W2071630659 cites W2000840258 @default.
• W2071630659 cites W2004330902 @default.
• W2071630659 cites W2008585649 @default.
• W2071630659 cites W2008708467 @default.
• W2071630659 cites W2023238772 @default.
• W2071630659 cites W2023717652 @default.
• W2071630659 cites W2029882922 @default.
• W2071630659 cites W2030337863 @default.
• W2071630659 cites W2035015160 @default.
• W2071630659 cites W2035199159 @default.
• W2071630659 cites W2040982165 @default.
• W2071630659 cites W2042299681 @default.
• W2071630659 cites W2044361715 @default.
• W2071630659 cites W2044713411 @default.
• W2071630659 cites W2045973688 @default.
• W2071630659 cites W2051293563 @default.
• W2071630659 cites W2053584679 @default.
• W2071630659 cites W2068243799 @default.
• W2071630659 cites W2071077620 @default.
• W2071630659 cites W2071319158 @default.
• W2071630659 cites W2072342298 @default.
• W2071630659 cites W2073130207 @default.
• W2071630659 cites W2073693436 @default.
• W2071630659 cites W2075505388 @default.
• W2071630659 cites W2076430296 @default.
• W2071630659 cites W2078971466 @default.
• W2071630659 cites W2079606633 @default.
• W2071630659 cites W2082393524 @default.
• W2071630659 cites W2089434536 @default.
• W2071630659 cites W2090338919 @default.
• W2071630659 cites W2091765441 @default.
• W2071630659 cites W2093381148 @default.
• W2071630659 cites W2109184569 @default.
• W2071630659 cites W2114320763 @default.
• W2071630659 cites W2117728222 @default.
• W2071630659 cites W2122199548 @default.
• W2071630659 cites W2131625617 @default.
• W2071630659 cites W2137745009 @default.
• W2071630659 cites W2144081223 @default.
• W2071630659 cites W2149038136 @default.
• W2071630659 cites W2149313395 @default.
• W2071630659 cites W2169821755 @default.
• W2071630659 doi "https://doi.org/10.1002/cbic.200900773" @default.
• W2071630659 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20349493" @default.
• W2071630659 hasPublicationYear "2010" @default.
• W2071630659 type Work @default.
• W2071630659 sameAs 2071630659 @default.
• W2071630659 citedByCount "22" @default.
• W2071630659 countsByYear W20716306592012 @default.
• W2071630659 countsByYear W20716306592013 @default.
• W2071630659 countsByYear W20716306592014 @default.
• W2071630659 countsByYear W20716306592017 @default.
• W2071630659 countsByYear W20716306592021 @default.
• W2071630659 countsByYear W20716306592022 @default.
• W2071630659 countsByYear W20716306592023 @default.
• W2071630659 crossrefType "journal-article" @default.
• W2071630659 hasAuthorship W2071630659A5008646033 @default.
• W2071630659 hasAuthorship W2071630659A5026624370 @default.
• W2071630659 hasAuthorship W2071630659A5027275474 @default.
• W2071630659 hasAuthorship W2071630659A5029954648 @default.
• W2071630659 hasAuthorship W2071630659A5030019260 @default.
• W2071630659 hasAuthorship W2071630659A5041481556 @default.
• W2071630659 hasAuthorship W2071630659A5065393453 @default.
• W2071630659 hasAuthorship W2071630659A5073272119 @default.
• W2071630659 hasAuthorship W2071630659A5074351885 @default.
• W2071630659 hasConcept C104317684 @default.
• W2071630659 hasConcept C119599485 @default.
• W2071630659 hasConcept C121332964 @default.
• W2071630659 hasConcept C127413603 @default.

• next