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- W2071707051 abstract "<h2>Abstract</h2> Because of the favourable effect of glycerol as a cryoprotective agent for the preservation of erythrocytes in the frozen state, the effect of glycerol on metabolism and functional capacity of platelets was examined to determine if this substance could also be used for the preservation of platelets. Platelet functions in the presence and absence of glycerol were studied by means of the ADP- and adrenaline-induced aggregation (BORN-test). In the presence of glycerol, higher ATP-levels were found in the platelets than in the controls, thus indicating a membrane stabilizing effect of glycerol. A maximal stabilization was obtained with 0.1 – 0.5 M glycerol. The membrane stabilizing effect of 0.3 M glycerol was paralleled by a 54% inhibition of ADP-induced aggregation and a 70% inhibition of adrenaline-induced aggregation. The spreading capacity of the platelets, which depends on an intact metabolism, decreased with increasing glycerol concentration. A 20% inhibition of the spreading capacity was obtained with 1.0 M glycerol. Platelet sediments prepared from platelet-rich plasma in the presence of glycerol could not be resuspended as readily as sediments prepared from pure platelet-rich plasma in the absence of glycerol." @default.
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- W2071707051 date "1975-06-01" @default.
- W2071707051 modified "2023-09-27" @default.
- W2071707051 title "Investigations on the preservation of human platelets I. The effect of glycerol on platelet metabolism and function" @default.
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- W2071707051 doi "https://doi.org/10.1016/0049-3848(75)90058-4" @default.
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