FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071715768> ?p ?o ?g. }

• W2071715768 abstract "Epigenetics describes the heritable changes in gene function that occur independently to the DNA sequence. The molecular basis of epigenetic gene regulation is complex, but essentially involves modifications to the DNA itself or the proteins with which DNA associates. The predominant epigenetic modification of DNA in mammalian genomes is methylation of cytosine nucleotides (5-MeC). DNA methylation provides instruction to gene expression machinery as to where and when the gene should be expressed. The primary target sequence for DNA methylation in mammals is 5'-CpG-3' dinucleotides (Figure 1). CpG dinucleotides are not uniformly distributed throughout the genome, but are concentrated in regions of repetitive genomic sequences and CpG islands commonly associated with gene promoters (Figure 1). DNA methylation patterns are established early in development, modulated during tissue specific differentiation and disrupted in many disease states including cancer. To understand the biological role of DNA methylation and its role in human disease, precise, efficient and reproducible methods are required to detect and quantify individual 5-MeCs. This protocol for bisulphite conversion is the gold standard for DNA methylation analysis and facilitates identification and quantification of DNA methylation at single nucleotide resolution. The chemistry of cytosine deamination by sodium bisulphite involves three steps (Figure 2). (1) Sulphonation: The addition of bisulphite to the 5-6 double bond of cytosine (2) Hydrolic Deamination: hydrolytic deamination of the resulting cytosine-bisulphite derivative to give a uracil-bisulphite derivative (3) Alkali Desulphonation: Removal of the sulphonate group by an alkali treatment, to give uracil. Bisulphite preferentially deaminates cytosine to uracil in single stranded DNA, whereas 5-MeC, is refractory to bisulphite-mediated deamination. Upon PCR amplification, uracil is amplified as thymine while 5-MeC residues remain as cytosines, allowing methylated CpGs to be distinguished from unmethylated CpGs by presence of a cytosine C versus thymine T residue during sequencing. DNA modification by bisulphite conversion is a well-established protocol that can be exploited for many methods of DNA methylation analysis. Since the detection of 5-MeC by bisulphite conversion was first demonstrated by Frommer et al. and Clark et al., methods based around bisulphite conversion of genomic DNA account for the majority of new data on DNA methylation. Different methods of post PCR analysis may be utilized, depending on the degree of specificity and resolution of methylation required. Cloning and sequencing is still the most readily available method that can give single nucleotide resolution for methylation across the DNA molecule." @default.
• W2071715768 created "2016-06-24" @default.
• W2071715768 creator A5005951713 @default.
• W2071715768 creator A5016423170 @default.
• W2071715768 creator A5041095680 @default.
• W2071715768 creator A5068810437 @default.
• W2071715768 date "2011-10-21" @default.
• W2071715768 modified "2023-10-17" @default.
• W2071715768 title "DNA Methylation: Bisulphite Modification and Analysis" @default.
• W2071715768 cites W1541216017 @default.
• W2071715768 cites W1589013348 @default.
• W2071715768 cites W183213617 @default.
• W2071715768 cites W1868185103 @default.
• W2071715768 cites W1966546601 @default.
• W2071715768 cites W1966707975 @default.
• W2071715768 cites W1968263601 @default.
• W2071715768 cites W1969014507 @default.
• W2071715768 cites W1969073965 @default.
• W2071715768 cites W1970288624 @default.
• W2071715768 cites W1975364471 @default.
• W2071715768 cites W1979273476 @default.
• W2071715768 cites W1994465394 @default.
• W2071715768 cites W2002006058 @default.
• W2071715768 cites W2019516356 @default.
• W2071715768 cites W2023280439 @default.
• W2071715768 cites W2025312397 @default.
• W2071715768 cites W2029683459 @default.
• W2071715768 cites W2033244804 @default.
• W2071715768 cites W2049174408 @default.
• W2071715768 cites W2051974123 @default.
• W2071715768 cites W2063110349 @default.
• W2071715768 cites W2072204379 @default.
• W2071715768 cites W2075106532 @default.
• W2071715768 cites W2076234267 @default.
• W2071715768 cites W2078144419 @default.
• W2071715768 cites W2082550660 @default.
• W2071715768 cites W2082777953 @default.
• W2071715768 cites W2087651356 @default.
• W2071715768 cites W2096287539 @default.
• W2071715768 cites W2098192588 @default.
• W2071715768 cites W2098362718 @default.
• W2071715768 cites W2106680700 @default.
• W2071715768 cites W2107081820 @default.
• W2071715768 cites W2114168826 @default.
• W2071715768 cites W2127829299 @default.
• W2071715768 cites W2128062614 @default.
• W2071715768 cites W2133596460 @default.
• W2071715768 cites W2133841021 @default.
• W2071715768 cites W2135157239 @default.
• W2071715768 cites W2139122477 @default.
• W2071715768 cites W2144131970 @default.
• W2071715768 cites W2144430600 @default.
• W2071715768 cites W2146614883 @default.
• W2071715768 cites W2147329127 @default.
• W2071715768 cites W2148405421 @default.
• W2071715768 cites W2158194917 @default.
• W2071715768 cites W2158470091 @default.
• W2071715768 cites W2164326183 @default.
• W2071715768 cites W2165266865 @default.
• W2071715768 cites W2182868165 @default.
• W2071715768 doi "https://doi.org/10.3791/3170" @default.
• W2071715768 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3227193" @default.
• W2071715768 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22042230" @default.
• W2071715768 hasPublicationYear "2011" @default.
• W2071715768 type Work @default.
• W2071715768 sameAs 2071715768 @default.
• W2071715768 citedByCount "46" @default.
• W2071715768 countsByYear W20717157682012 @default.
• W2071715768 countsByYear W20717157682013 @default.
• W2071715768 countsByYear W20717157682014 @default.
• W2071715768 countsByYear W20717157682015 @default.
• W2071715768 countsByYear W20717157682016 @default.
• W2071715768 countsByYear W20717157682017 @default.
• W2071715768 countsByYear W20717157682018 @default.
• W2071715768 countsByYear W20717157682019 @default.
• W2071715768 countsByYear W20717157682020 @default.
• W2071715768 countsByYear W20717157682021 @default.
• W2071715768 countsByYear W20717157682022 @default.
• W2071715768 countsByYear W20717157682023 @default.
• W2071715768 crossrefType "journal-article" @default.
• W2071715768 hasAuthorship W2071715768A5005951713 @default.
• W2071715768 hasAuthorship W2071715768A5016423170 @default.
• W2071715768 hasAuthorship W2071715768A5041095680 @default.
• W2071715768 hasAuthorship W2071715768A5068810437 @default.
• W2071715768 hasBestOaLocation W20717157681 @default.
• W2071715768 hasConcept C104317684 @default.
• W2071715768 hasConcept C116114238 @default.
• W2071715768 hasConcept C136953436 @default.
• W2071715768 hasConcept C140173407 @default.
• W2071715768 hasConcept C145782189 @default.
• W2071715768 hasConcept C150194340 @default.
• W2071715768 hasConcept C153911025 @default.
• W2071715768 hasConcept C181199279 @default.
• W2071715768 hasConcept C185592680 @default.
• W2071715768 hasConcept C189235521 @default.
• W2071715768 hasConcept C190727270 @default.
• W2071715768 hasConcept C2775926025 @default.
• W2071715768 hasConcept C2776023528 @default.
• W2071715768 hasConcept C33288867 @default.
• W2071715768 hasConcept C37080517 @default.

• next