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- W2071738951 abstract "Nitrogenase from cell-free extracts of bacteriods prepared from soybean root nodules has been resolved by anaerobic gel filtration chromatography into two components. The first, with an apparent molecular weight of 180 000 from V e data, contained iron and molybdenum; fractions containing it comprised approx. 6.5% of the bacteroid soluble protein and had a maximum specific activity of 443 nmoles/min per mg protein by the acetylene reduction assay. The second component contained iron and had an apparent molecular weight of 50 000; fractions containing it comprised about 12% of the bacteroid soluble protein and had a maximum specific activity of 71 nmoles/min per mg. Freshly prepared bacteroid extracts were found to be deficient in the second component of nitrogenase." @default.
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- W2071738951 date "1970-07-01" @default.
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- W2071738951 title "Gel filtration of nitrogenase from soybean root-nodule bacteroids" @default.
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- W2071738951 doi "https://doi.org/10.1016/0005-2795(70)90066-8" @default.
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