FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071769488> ?p ?o ?g. }

• W2071769488 endingPage "11096" @default.
• W2071769488 startingPage "11091" @default.
• W2071769488 abstract "L-type Ca 2+ currents determine the shape of cardiac action potentials (AP) and the magnitude of the myoplasmic Ca 2+ signal, which regulates the contraction force. The auxiliary Ca 2+ channel subunits α 2 δ-1 and β 2 are important regulators of membrane expression and current properties of the cardiac Ca 2+ channel (Ca V 1.2). However, their role in cardiac excitation–contraction coupling is still elusive. Here we addressed this question by combining siRNA knockdown of the α 2 δ-1 subunit in a muscle expression system with simulation of APs and Ca 2+ transients by using a quantitative computer model of ventricular myocytes. Reconstitution of dysgenic muscle cells with Ca V 1.2 (GFP-α 1C ) recapitulates key properties of cardiac excitation–contraction coupling. Concomitant depletion of the α 2 δ-1 subunit did not perturb membrane expression or targeting of the pore-forming GFP-α 1C subunit into junctions between the outer membrane and the sarcoplasmic reticulum. However, α 2 δ-1 depletion shifted the voltage dependence of Ca 2+ current activation by 9 mV to more positive potentials, and it slowed down activation and inactivation kinetics approximately 2-fold. Computer modeling revealed that the altered voltage dependence and current kinetics exert opposing effects on the function of ventricular myocytes that in total cause a 60% prolongation of the AP and a 2-fold increase of the myoplasmic Ca 2+ concentration during each contraction. Thus, the Ca 2+ channel α 2 δ-1 subunit is not essential for normal Ca 2+ channel targeting in muscle but is a key determinant of normal excitation and contraction of cardiac muscle cells, and a reduction of α 2 δ-1 function is predicted to severely perturb normal heart function." @default.
• W2071769488 created "2016-06-24" @default.
• W2071769488 creator A5006384137 @default.
• W2071769488 creator A5040730141 @default.
• W2071769488 creator A5050650686 @default.
• W2071769488 creator A5051886871 @default.
• W2071769488 date "2007-06-26" @default.
• W2071769488 modified "2023-10-02" @default.
• W2071769488 title "Computer modeling of siRNA knockdown effects indicates an essential role of the Ca ²⁺ channel α ₂ δ-1 subunit in cardiac excitation–contraction coupling" @default.
• W2071769488 cites W1570544530 @default.
• W2071769488 cites W1604986533 @default.
• W2071769488 cites W1902878432 @default.
• W2071769488 cites W1982140992 @default.
• W2071769488 cites W1995550177 @default.
• W2071769488 cites W1998672281 @default.
• W2071769488 cites W2002546976 @default.
• W2071769488 cites W2016321175 @default.
• W2071769488 cites W2016547597 @default.
• W2071769488 cites W2019028325 @default.
• W2071769488 cites W2037100068 @default.
• W2071769488 cites W2048497026 @default.
• W2071769488 cites W2059372092 @default.
• W2071769488 cites W2062831895 @default.
• W2071769488 cites W2076930890 @default.
• W2071769488 cites W2078971375 @default.
• W2071769488 cites W2088072568 @default.
• W2071769488 cites W2106109601 @default.
• W2071769488 cites W2113564988 @default.
• W2071769488 cites W2125194963 @default.
• W2071769488 cites W2138773108 @default.
• W2071769488 cites W2156263771 @default.
• W2071769488 cites W2160862437 @default.
• W2071769488 cites W2165445451 @default.
• W2071769488 cites W2169550464 @default.
• W2071769488 cites W4322703181 @default.
• W2071769488 doi "https://doi.org/10.1073/pnas.0700577104" @default.
• W2071769488 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1904133" @default.
• W2071769488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17563358" @default.
• W2071769488 hasPublicationYear "2007" @default.
• W2071769488 type Work @default.
• W2071769488 sameAs 2071769488 @default.
• W2071769488 citedByCount "46" @default.
• W2071769488 countsByYear W20717694882012 @default.
• W2071769488 countsByYear W20717694882013 @default.
• W2071769488 countsByYear W20717694882014 @default.
• W2071769488 countsByYear W20717694882015 @default.
• W2071769488 countsByYear W20717694882016 @default.
• W2071769488 countsByYear W20717694882017 @default.
• W2071769488 countsByYear W20717694882018 @default.
• W2071769488 countsByYear W20717694882019 @default.
• W2071769488 countsByYear W20717694882020 @default.
• W2071769488 countsByYear W20717694882021 @default.
• W2071769488 countsByYear W20717694882022 @default.
• W2071769488 countsByYear W20717694882023 @default.
• W2071769488 crossrefType "journal-article" @default.
• W2071769488 hasAuthorship W2071769488A5006384137 @default.
• W2071769488 hasAuthorship W2071769488A5040730141 @default.
• W2071769488 hasAuthorship W2071769488A5050650686 @default.
• W2071769488 hasAuthorship W2071769488A5051886871 @default.
• W2071769488 hasBestOaLocation W20717694882 @default.
• W2071769488 hasConcept C104292427 @default.
• W2071769488 hasConcept C104317684 @default.
• W2071769488 hasConcept C12554922 @default.
• W2071769488 hasConcept C131584629 @default.
• W2071769488 hasConcept C134018914 @default.
• W2071769488 hasConcept C158617107 @default.
• W2071769488 hasConcept C163415756 @default.
• W2071769488 hasConcept C173396325 @default.
• W2071769488 hasConcept C181911157 @default.
• W2071769488 hasConcept C185592680 @default.
• W2071769488 hasConcept C190283241 @default.
• W2071769488 hasConcept C191897082 @default.
• W2071769488 hasConcept C192562407 @default.
• W2071769488 hasConcept C207200792 @default.
• W2071769488 hasConcept C2778996579 @default.
• W2071769488 hasConcept C55493867 @default.
• W2071769488 hasConcept C86803240 @default.
• W2071769488 hasConcept C95444343 @default.
• W2071769488 hasConceptScore W2071769488C104292427 @default.
• W2071769488 hasConceptScore W2071769488C104317684 @default.
• W2071769488 hasConceptScore W2071769488C12554922 @default.
• W2071769488 hasConceptScore W2071769488C131584629 @default.
• W2071769488 hasConceptScore W2071769488C134018914 @default.
• W2071769488 hasConceptScore W2071769488C158617107 @default.
• W2071769488 hasConceptScore W2071769488C163415756 @default.
• W2071769488 hasConceptScore W2071769488C173396325 @default.
• W2071769488 hasConceptScore W2071769488C181911157 @default.
• W2071769488 hasConceptScore W2071769488C185592680 @default.
• W2071769488 hasConceptScore W2071769488C190283241 @default.
• W2071769488 hasConceptScore W2071769488C191897082 @default.
• W2071769488 hasConceptScore W2071769488C192562407 @default.
• W2071769488 hasConceptScore W2071769488C207200792 @default.
• W2071769488 hasConceptScore W2071769488C2778996579 @default.
• W2071769488 hasConceptScore W2071769488C55493867 @default.
• W2071769488 hasConceptScore W2071769488C86803240 @default.
• W2071769488 hasConceptScore W2071769488C95444343 @default.
• W2071769488 hasIssue "26" @default.
• W2071769488 hasLocation W20717694881 @default.

• next