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- W2071771574 endingPage "e0117225" @default.
- W2071771574 startingPage "e0117225" @default.
- W2071771574 abstract "Light microscopy enables noninvasive imaging of fluorescent species in biological specimens, but resolution is generally limited by diffraction to ~200–250 nm. Many biological processes occur on smaller length scales, highlighting the importance of techniques that can image below the diffraction limit and provide valuable single-molecule information. In recent years, imaging techniques have been developed which can achieve resolution below the diffraction limit. Utilizing one such technique, fluorescence photoactivation localization microscopy (FPALM), we demonstrated its ability to construct super-resolution images from single molecules in a living zebrafish embryo, expanding the realm of previous super-resolution imaging to a living vertebrate organism. We imaged caveolin-1 in vivo, in living zebrafish embryos. Our results demonstrate the successful image acquisition of super-resolution images in a living vertebrate organism, opening several opportunities to answer more dynamic biological questions in vivo at the previously inaccessible nanoscale." @default.
- W2071771574 created "2016-06-24" @default.
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- W2071771574 date "2015-02-03" @default.
- W2071771574 modified "2023-09-27" @default.
- W2071771574 title "Nanoscale Imaging of Caveolin-1 Membrane Domains In Vivo" @default.
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- W2071771574 doi "https://doi.org/10.1371/journal.pone.0117225" @default.
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