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- W2071774174 abstract "For enzymatic hydrolyses of advanced synthetic intermediates, the use of an organic cosolvent is often warranted. As a model study, the cosolvent dependence (at 10 volume%) of the key enzymatic step in our asymmetric synthesis of (−)-podophyllotoxin was examined. At high protein concentrations and rt, DMSO and the polyethereal organic cosolvents dioxane, diglyme and triglyme emerged as the best organic cosolvents, in terms of yield, % ee and catalytic efficiency (Vmax/Km). However, only in 10% triglyme was optimal efficiency maintained at both lower protein concentration and higher temperature." @default.
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- W2071774174 date "1999-12-01" @default.
- W2071774174 modified "2023-09-24" @default.
- W2071774174 title "Hydrolytic enzymatic transformation of advanced synthetic intermediates: on the choice of the organic cosolvent" @default.
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- W2071774174 doi "https://doi.org/10.1016/s0957-4166(99)00509-1" @default.
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