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- W2071776158 abstract "Au cours de la mise au point d'une méthode immunoenzymatique de dépistage des Escherichia coli porteurs d'adhésines CFA/I ou CFA/II dans des selles diarrhéiques, la méthode de fixation des anticorps sur le polystyrène par covalence a été comparée à l'adsorption passive. La fixation covalente des anticorps par covalence permet de conserver les plaques 9 semaines au lieu de 6 par simple adsorption. La sensibilité pour les deux techniques est de 105 bactéries/ml pour l'antigène CFA/I et 106 pour le CFA/II. The enzyme linked immunosorbent assay to detect colonization factor antigens in feces is described. The fixation by passive adsorption or covalent binding of the anti-CFA/I and anti-CFA/II antibodies on the polystyrene of the plates of microtitration is studied. The covalent binding allow to conserve the reactivity of the plates 9 weeks instead of 6 with a passive adsorption. The sensitivity of the both methods are of 105 bacteria/ml for CFA/I and 106 bacteria/ml for CFA/II." @default.
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- W2071776158 date "1988-01-01" @default.
- W2071776158 modified "2023-09-28" @default.
- W2071776158 title "Etude de la realisation d'un immunoadsorbant par covalence pour le depistage immunoenzymatique dans les selles humaines des Escherichia coli porteurs des adhesines CFA/I ou CFA/II" @default.
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- W2071776158 doi "https://doi.org/10.1016/s0399-077x(88)80049-0" @default.
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