Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071788380> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2071788380 endingPage "3198" @default.
- W2071788380 startingPage "3193" @default.
- W2071788380 abstract "PRNP is the gene encoding prion protein whose misfolded and β-sheet-rich isoform is the infectious agent of transmissible spongiform encephalopathy (TSE). TSE, also called prion diseases, cause fatal neurodegenerative and transmissible disorders in human and animals. Among these diseases, bovine spongiform encephalopathy (BSE) has tremendous impact on economy and human health in the world. In the present study, we hypothesize suppression of the PRNP gene expression could raise resistance to BSE in cattle by using vector-based small interfering RNA (siRNA) expression systems. Therefore, the objective was to screen effective DNA-encoding short hairpin RNAs (shRNAs) which could knockdown the PRNP gene expression in bovine fibroblast cells. Human U6 promoter was employed to drive shRNA transcription from the DNA vector, and seven shRNAs, that designed to target coding region and 3′ untranslated region of the PRNP gene, were selected. Four out of seven shRNAs tested were found to be effective in inhibiting the PRNP gene expression, and the most significant suppression level was as much as 62.9% evidenced by real-time RT-PCR. Furthermore, the protein abundance was obviously reduced compared to the control. Overall, the present study demonstrated that vector-based siRNA expression systems is an efficient approach to knockdown the PRNP gene expression in bovine fibroblast cells and thereby provide donor cells for somatic cell nuclear cloning to produce cattle that is resistant to prion related diseases." @default.
- W2071788380 created "2016-06-24" @default.
- W2071788380 creator A5004235355 @default.
- W2071788380 creator A5031810848 @default.
- W2071788380 creator A5032341066 @default.
- W2071788380 creator A5036888170 @default.
- W2071788380 creator A5048965762 @default.
- W2071788380 creator A5052305380 @default.
- W2071788380 creator A5075869658 @default.
- W2071788380 creator A5086722810 @default.
- W2071788380 date "2009-10-11" @default.
- W2071788380 modified "2023-10-14" @default.
- W2071788380 title "Knockdown of the prion gene expression by RNA interference in bovine fibroblast cells" @default.
- W2071788380 cites W1488192195 @default.
- W2071788380 cites W1611419331 @default.
- W2071788380 cites W1972656052 @default.
- W2071788380 cites W1973673075 @default.
- W2071788380 cites W1974595102 @default.
- W2071788380 cites W1988967884 @default.
- W2071788380 cites W1992380356 @default.
- W2071788380 cites W1998904995 @default.
- W2071788380 cites W2000742874 @default.
- W2071788380 cites W2002615250 @default.
- W2071788380 cites W2042978244 @default.
- W2071788380 cites W2044004977 @default.
- W2071788380 cites W2056675674 @default.
- W2071788380 cites W2072244190 @default.
- W2071788380 cites W2086208402 @default.
- W2071788380 cites W2108775715 @default.
- W2071788380 cites W2118947905 @default.
- W2071788380 cites W2146900615 @default.
- W2071788380 cites W2147592113 @default.
- W2071788380 cites W2324660492 @default.
- W2071788380 doi "https://doi.org/10.1007/s11033-009-9900-0" @default.
- W2071788380 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19821149" @default.
- W2071788380 hasPublicationYear "2009" @default.
- W2071788380 type Work @default.
- W2071788380 sameAs 2071788380 @default.
- W2071788380 citedByCount "5" @default.
- W2071788380 countsByYear W20717883802012 @default.
- W2071788380 countsByYear W20717883802015 @default.
- W2071788380 crossrefType "journal-article" @default.
- W2071788380 hasAuthorship W2071788380A5004235355 @default.
- W2071788380 hasAuthorship W2071788380A5031810848 @default.
- W2071788380 hasAuthorship W2071788380A5032341066 @default.
- W2071788380 hasAuthorship W2071788380A5036888170 @default.
- W2071788380 hasAuthorship W2071788380A5048965762 @default.
- W2071788380 hasAuthorship W2071788380A5052305380 @default.
- W2071788380 hasAuthorship W2071788380A5075869658 @default.
- W2071788380 hasAuthorship W2071788380A5086722810 @default.
- W2071788380 hasBestOaLocation W20717883802 @default.
- W2071788380 hasConcept C104317684 @default.
- W2071788380 hasConcept C119056186 @default.
- W2071788380 hasConcept C150194340 @default.
- W2071788380 hasConcept C153911025 @default.
- W2071788380 hasConcept C159047783 @default.
- W2071788380 hasConcept C166703698 @default.
- W2071788380 hasConcept C173396325 @default.
- W2071788380 hasConcept C180754005 @default.
- W2071788380 hasConcept C190232843 @default.
- W2071788380 hasConcept C2778147599 @default.
- W2071788380 hasConcept C54355233 @default.
- W2071788380 hasConcept C67705224 @default.
- W2071788380 hasConcept C86803240 @default.
- W2071788380 hasConceptScore W2071788380C104317684 @default.
- W2071788380 hasConceptScore W2071788380C119056186 @default.
- W2071788380 hasConceptScore W2071788380C150194340 @default.
- W2071788380 hasConceptScore W2071788380C153911025 @default.
- W2071788380 hasConceptScore W2071788380C159047783 @default.
- W2071788380 hasConceptScore W2071788380C166703698 @default.
- W2071788380 hasConceptScore W2071788380C173396325 @default.
- W2071788380 hasConceptScore W2071788380C180754005 @default.
- W2071788380 hasConceptScore W2071788380C190232843 @default.
- W2071788380 hasConceptScore W2071788380C2778147599 @default.
- W2071788380 hasConceptScore W2071788380C54355233 @default.
- W2071788380 hasConceptScore W2071788380C67705224 @default.
- W2071788380 hasConceptScore W2071788380C86803240 @default.
- W2071788380 hasIssue "7" @default.
- W2071788380 hasLocation W20717883801 @default.
- W2071788380 hasLocation W20717883802 @default.
- W2071788380 hasLocation W20717883803 @default.
- W2071788380 hasOpenAccess W2071788380 @default.
- W2071788380 hasPrimaryLocation W20717883801 @default.
- W2071788380 hasRelatedWork W146197187 @default.
- W2071788380 hasRelatedWork W2031264259 @default.
- W2071788380 hasRelatedWork W2060001679 @default.
- W2071788380 hasRelatedWork W2088835981 @default.
- W2071788380 hasRelatedWork W2150505140 @default.
- W2071788380 hasRelatedWork W2162323474 @default.
- W2071788380 hasRelatedWork W2373539659 @default.
- W2071788380 hasRelatedWork W2413319726 @default.
- W2071788380 hasRelatedWork W2417676917 @default.
- W2071788380 hasRelatedWork W2508597590 @default.
- W2071788380 hasVolume "37" @default.
- W2071788380 isParatext "false" @default.
- W2071788380 isRetracted "false" @default.
- W2071788380 magId "2071788380" @default.
- W2071788380 workType "article" @default.