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• W2071816960 endingPage "2475" @default.
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• W2071816960 abstract "Rhodopsin is the best-understood member of the large G protein-coupled receptor (GPCR) superfamily. The G-protein amplification cascade is triggered by poorly understood light-induced conformational changes in rhodopsin that are homologous to changes caused by agonists in other GPCRs. We have applied the antibody imprint method to light-activated rhodopsin in native membranes by using nine monoclonal antibodies (mAbs) against aqueous faces of rhodopsin. Epitopes recognized by these mAbs were found by selection from random peptide libraries displayed on phage. A new computer algorithm, FINDMAP, was used to map the epitopes to discontinuous segments of rhodopsin that are distant in the primary sequence but are in close spatial proximity in the structure. The proximity of a segment of the N-terminal and the loop between helices VI and VIII found by FINDMAP is consistent with the X-ray structure of the dark-adapted rhodopsin. Epitopes to the cytoplasmic face segregated into two classes with different predicted spatial proximities of protein segments that correlate with different preferences of the antibodies for stabilizing the metarhodopsin I or metarhodopsin II conformations of light-excited rhodopsin. Epitopes of antibodies that stabilize metarhodopsin II indicate conformational changes from dark-adapted rhodopsin, including rearrangements of the C-terminal tail and altered exposure of the cytoplasmic end of helix VI, a portion of the C-3 loop, and helix VIII. As additional antibodies are subjected to antibody imprinting, this approach should provide increasingly detailed information on the conformation of light-excited rhodopsin and be applicable to structural studies of other challenging protein targets." @default.
• W2071816960 created "2016-06-24" @default.
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• W2071816960 date "2003-11-01" @default.
• W2071816960 modified "2023-10-09" @default.
• W2071816960 title "Constraints on the conformation of the cytoplasmic face of dark-adapted and light-excited rhodopsin inferred from antirhodopsin antibody imprints" @default.
• W2071816960 cites W1026154262 @default.
• W2071816960 cites W115878226 @default.
• W2071816960 cites W1481130100 @default.
• W2071816960 cites W1482237901 @default.
• W2071816960 cites W1497874406 @default.
• W2071816960 cites W1526151739 @default.
• W2071816960 cites W1526269615 @default.
• W2071816960 cites W1567745743 @default.
• W2071816960 cites W1591942886 @default.
• W2071816960 cites W1592870802 @default.
• W2071816960 cites W1594214770 @default.
• W2071816960 cites W160255425 @default.
• W2071816960 cites W1799572558 @default.
• W2071816960 cites W1858237937 @default.
• W2071816960 cites W1969303095 @default.
• W2071816960 cites W1970736649 @default.
• W2071816960 cites W1974597502 @default.
• W2071816960 cites W1975558038 @default.
• W2071816960 cites W1975960076 @default.
• W2071816960 cites W1978727971 @default.
• W2071816960 cites W1980645803 @default.
• W2071816960 cites W1980823970 @default.
• W2071816960 cites W1981619807 @default.
• W2071816960 cites W1981916724 @default.
• W2071816960 cites W1982581618 @default.
• W2071816960 cites W1983635965 @default.
• W2071816960 cites W1983822014 @default.
• W2071816960 cites W1986402769 @default.
• W2071816960 cites W1988787099 @default.
• W2071816960 cites W1989389355 @default.
• W2071816960 cites W1989764592 @default.
• W2071816960 cites W1994501723 @default.
• W2071816960 cites W1995804959 @default.
• W2071816960 cites W2003947345 @default.
• W2071816960 cites W2010194803 @default.
• W2071816960 cites W2010649076 @default.
• W2071816960 cites W2012638283 @default.
• W2071816960 cites W2014576184 @default.
• W2071816960 cites W2015176049 @default.
• W2071816960 cites W2015642465 @default.
• W2071816960 cites W2016441306 @default.
• W2071816960 cites W2018376949 @default.
• W2071816960 cites W2023038974 @default.
• W2071816960 cites W2026991751 @default.
• W2071816960 cites W2027408247 @default.
• W2071816960 cites W2031543235 @default.
• W2071816960 cites W2034890791 @default.
• W2071816960 cites W2041208984 @default.
• W2071816960 cites W2042170041 @default.
• W2071816960 cites W2052152476 @default.
• W2071816960 cites W2056139298 @default.
• W2071816960 cites W2057543968 @default.
• W2071816960 cites W2060654606 @default.
• W2071816960 cites W2065143804 @default.
• W2071816960 cites W2066180439 @default.
• W2071816960 cites W2067104956 @default.
• W2071816960 cites W2073110681 @default.
• W2071816960 cites W2075770210 @default.
• W2071816960 cites W2082006283 @default.
• W2071816960 cites W2082611727 @default.
• W2071816960 cites W2083445427 @default.
• W2071816960 cites W2084429764 @default.
• W2071816960 cites W2094747541 @default.
• W2071816960 cites W2095572398 @default.
• W2071816960 cites W2103967018 @default.
• W2071816960 cites W2111297610 @default.
• W2071816960 cites W2113585001 @default.
• W2071816960 cites W2122259597 @default.
• W2071816960 cites W2124961362 @default.
• W2071816960 cites W2129579237 @default.
• W2071816960 cites W2130049582 @default.
• W2071816960 cites W2130479394 @default.
• W2071816960 cites W2130920644 @default.
• W2071816960 cites W2133524076 @default.
• W2071816960 cites W2135176361 @default.
• W2071816960 cites W2142779916 @default.
• W2071816960 cites W2149963584 @default.
• W2071816960 cites W21816278 @default.
• W2071816960 cites W2323899541 @default.
• W2071816960 cites W4251074911 @default.
• W2071816960 cites W4251828057 @default.
• W2071816960 doi "https://doi.org/10.1110/ps.03233703" @default.
• W2071816960 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2366960" @default.

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