Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071827167> ?p ?o ?g. }
- W2071827167 endingPage "1177" @default.
- W2071827167 startingPage "1168" @default.
- W2071827167 abstract "ABSTRACT Mature human immunodeficiency virus type 1 (HIV-1) particles contain a cone-shaped core structure consisting of the internal ribonucleoprotein complex encased in a proteinaceous shell derived from the viral capsid protein. Because of their very low stability after membrane removal, HIV-1 cores have not been purified in quantities sufficient for structural and biochemical analysis. Based on our in vitro assembly experiments, we have developed a novel method for isolation of intact mature HIV-1 cores. Concentrated virus suspensions were briefly treated with nonionic detergent and immediately centrifuged in a microcentrifuge for short periods of time. The resuspended pellet was subsequently analyzed by negative-stain and thin-section electron microscopy and by immunoelectron microscopy. Abundant cone-shaped cores as well as tubular and aberrant structures were observed. Stereo images showed that core structures preserved their three-dimensional architecture and exhibited a regular substructure. Detailed analysis of 155 cores revealed an average length of ca. 103 nm, an average diameter at the base of ca. 52 nm, and an average angle of 21.3°. There was significant variability in all parameters, indicating that HIV cores are not homogeneous. Immunoblot analysis of core preparations allowed semiquantitative estimation of the relative amounts of viral and cellular proteins inside the HIV-1 core, yielding a model for the topology of various proteins inside the virion." @default.
- W2071827167 created "2016-06-24" @default.
- W2071827167 creator A5015447564 @default.
- W2071827167 creator A5030856723 @default.
- W2071827167 creator A5036005209 @default.
- W2071827167 creator A5038844784 @default.
- W2071827167 creator A5082177117 @default.
- W2071827167 date "2000-02-01" @default.
- W2071827167 modified "2023-10-16" @default.
- W2071827167 title "Biochemical and Structural Analysis of Isolated Mature Cores of Human Immunodeficiency Virus Type 1" @default.
- W2071827167 cites W1507261754 @default.
- W2071827167 cites W1528266646 @default.
- W2071827167 cites W1546130128 @default.
- W2071827167 cites W1571845954 @default.
- W2071827167 cites W1573886646 @default.
- W2071827167 cites W1579792152 @default.
- W2071827167 cites W1608128491 @default.
- W2071827167 cites W1630981685 @default.
- W2071827167 cites W1659719610 @default.
- W2071827167 cites W1664638011 @default.
- W2071827167 cites W1775967318 @default.
- W2071827167 cites W1866735636 @default.
- W2071827167 cites W1878167671 @default.
- W2071827167 cites W1900666052 @default.
- W2071827167 cites W1924577704 @default.
- W2071827167 cites W1941217386 @default.
- W2071827167 cites W1967406495 @default.
- W2071827167 cites W1969819429 @default.
- W2071827167 cites W1974956422 @default.
- W2071827167 cites W1975085160 @default.
- W2071827167 cites W1984806569 @default.
- W2071827167 cites W1985730918 @default.
- W2071827167 cites W1986232807 @default.
- W2071827167 cites W1995407171 @default.
- W2071827167 cites W2001243226 @default.
- W2071827167 cites W2015326137 @default.
- W2071827167 cites W2017190280 @default.
- W2071827167 cites W2020597127 @default.
- W2071827167 cites W2024534168 @default.
- W2071827167 cites W2028400386 @default.
- W2071827167 cites W2031739459 @default.
- W2071827167 cites W203606356 @default.
- W2071827167 cites W2039731241 @default.
- W2071827167 cites W2043467643 @default.
- W2071827167 cites W2050598058 @default.
- W2071827167 cites W2061714407 @default.
- W2071827167 cites W2062334646 @default.
- W2071827167 cites W2067109667 @default.
- W2071827167 cites W2073178398 @default.
- W2071827167 cites W2076634735 @default.
- W2071827167 cites W2076811542 @default.
- W2071827167 cites W2077455094 @default.
- W2071827167 cites W2078205352 @default.
- W2071827167 cites W2078960005 @default.
- W2071827167 cites W2083895131 @default.
- W2071827167 cites W2088458538 @default.
- W2071827167 cites W2092990233 @default.
- W2071827167 cites W2097074859 @default.
- W2071827167 cites W2099434032 @default.
- W2071827167 cites W2103069739 @default.
- W2071827167 cites W2103796054 @default.
- W2071827167 cites W2143430925 @default.
- W2071827167 cites W2144102385 @default.
- W2071827167 cites W2146187278 @default.
- W2071827167 cites W2150469985 @default.
- W2071827167 cites W2153097311 @default.
- W2071827167 cites W2153788348 @default.
- W2071827167 cites W2158213710 @default.
- W2071827167 cites W2169408873 @default.
- W2071827167 cites W2248336029 @default.
- W2071827167 cites W2467522500 @default.
- W2071827167 doi "https://doi.org/10.1128/jvi.74.3.1168-1177.2000" @default.
- W2071827167 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/111451" @default.
- W2071827167 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10627527" @default.
- W2071827167 hasPublicationYear "2000" @default.
- W2071827167 type Work @default.
- W2071827167 sameAs 2071827167 @default.
- W2071827167 citedByCount "188" @default.
- W2071827167 countsByYear W20718271672012 @default.
- W2071827167 countsByYear W20718271672013 @default.
- W2071827167 countsByYear W20718271672014 @default.
- W2071827167 countsByYear W20718271672015 @default.
- W2071827167 countsByYear W20718271672016 @default.
- W2071827167 countsByYear W20718271672017 @default.
- W2071827167 countsByYear W20718271672018 @default.
- W2071827167 countsByYear W20718271672019 @default.
- W2071827167 countsByYear W20718271672020 @default.
- W2071827167 countsByYear W20718271672021 @default.
- W2071827167 countsByYear W20718271672022 @default.
- W2071827167 countsByYear W20718271672023 @default.
- W2071827167 crossrefType "journal-article" @default.
- W2071827167 hasAuthorship W2071827167A5015447564 @default.
- W2071827167 hasAuthorship W2071827167A5030856723 @default.
- W2071827167 hasAuthorship W2071827167A5036005209 @default.
- W2071827167 hasAuthorship W2071827167A5038844784 @default.
- W2071827167 hasAuthorship W2071827167A5082177117 @default.
- W2071827167 hasBestOaLocation W20718271671 @default.
- W2071827167 hasConcept C120665830 @default.