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• W2071894906 abstract "Three phase partitioning (TPP), an efficient bioseparation technique, was used to purify nattokinase from fermentation broth of Bacillus natto NRRL-3666. TPP is a relatively recent technique that uses combination of ammonium sulphate and t-butanol to precipitate protein from crude extracts. The precipitated protein forms interface between the lower aqueous layer and the upper organic layer. The key parameters that were studied for efficient purification were temperature, pH, concentration of ammonium sulphate and t-butanol. The pH of 8, temperature of 37 °C, ammonium sulphate 30% (w/v) and ratio of crude extract to t-butanol 1:1.5 gave the most desired results. A single stage TPP led to 5.6-fold purification with an activity recovery of 129.5%. Nattokinase was freeze dried by using different excipients. There was no effect on the secondary structure of nattokinase while using t-butanol (co-solvent) for freeze drying. But there was reduction in drying time as well as the nattokinase cake became more porous." @default.
• W2071894906 created "2016-06-24" @default.
• W2071894906 creator A5039717855 @default.
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• W2071894906 date "2014-06-01" @default.
• W2071894906 modified "2023-10-11" @default.
• W2071894906 title "Nattokinase purification by three phase partitioning and impact of t-butanol on freeze drying" @default.
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• W2071894906 doi "https://doi.org/10.1016/j.seppur.2014.04.011" @default.
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