FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071897480> ?p ?o ?g. }

• W2071897480 endingPage "161" @default.
• W2071897480 startingPage "153" @default.
• W2071897480 abstract "Aquaporins (AQPs) are a family of channels permeable to water and some small solutes. In mammals, 13 members (AQP0-AQP12) have been found. AQP8 is widely distributed in many tissues and organs. Previous studies in frog oocytes suggested that AQP8 was permeable to water, urea and ammonium, but no direct characterization had yet been reported.We expressed recombinant rAQP8, hAQP8 and mAQP8 (rat, human and mouse AQP8 respectively) in yeast, purified the proteins to homogeneity and reconstituted them into proteoliposomes. Although showing high sequence similarity, AQP8 proteins from the three species had to be purified with different detergents prior to reconstitution. In stopped-flow studies, all three AQP8 proteoliposomes showed water permeability, which was inhibited by mercuric chloride and rescued by 2-mercaptoethanol. rAQP8 and hAQP8 proteoliposomes did not transport glycerol or urea but were permeable to formamide, which was also inhibited by mercuric chloride. In the oocyte transport assay, hAQP8-injected oocytes showed significantly higher [14C]methylammonium uptake than water-injected oocytes.In the present study, we successfully purified rAQP8, hAQP8 and mAQP8 proteins and characterized their biochemical and biophysical properties. All three AQP8 proteins transport water. rAQP8 and hAQP8 are not permeable to urea or glycerol. Moreover, hAQP8 is permeable to ammonium analogues (formamide and methylammonium). Our results suggest that AQP8 may transport ammonium in vivo and physiologically contribute to the acid-base equilibrium." @default.
• W2071897480 created "2016-06-24" @default.
• W2071897480 creator A5017989879 @default.
• W2071897480 creator A5041024027 @default.
• W2071897480 creator A5072803870 @default.
• W2071897480 creator A5088903163 @default.
• W2071897480 creator A5077975447 @default.
• W2071897480 date "2006-03-01" @default.
• W2071897480 modified "2023-10-10" @default.
• W2071897480 title "Purification and functional characterization of aquaporin-8" @default.
• W2071897480 cites W1479762169 @default.
• W2071897480 cites W1985938141 @default.
• W2071897480 cites W1987371378 @default.
• W2071897480 cites W1988357787 @default.
• W2071897480 cites W1995572705 @default.
• W2071897480 cites W1998548799 @default.
• W2071897480 cites W1999340580 @default.
• W2071897480 cites W2003879331 @default.
• W2071897480 cites W2010182284 @default.
• W2071897480 cites W2011357793 @default.
• W2071897480 cites W2020669819 @default.
• W2071897480 cites W2022231200 @default.
• W2071897480 cites W2027153868 @default.
• W2071897480 cites W2028556330 @default.
• W2071897480 cites W2046419770 @default.
• W2071897480 cites W2052922251 @default.
• W2071897480 cites W2061678845 @default.
• W2071897480 cites W2093371144 @default.
• W2071897480 cites W2095251996 @default.
• W2071897480 cites W2096526128 @default.
• W2071897480 cites W2110015614 @default.
• W2071897480 cites W2151427090 @default.
• W2071897480 doi "https://doi.org/10.1042/bc20050026" @default.
• W2071897480 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15948717" @default.
• W2071897480 hasPublicationYear "2006" @default.
• W2071897480 type Work @default.
• W2071897480 sameAs 2071897480 @default.
• W2071897480 citedByCount "93" @default.
• W2071897480 countsByYear W20718974802012 @default.
• W2071897480 countsByYear W20718974802013 @default.
• W2071897480 countsByYear W20718974802014 @default.
• W2071897480 countsByYear W20718974802015 @default.
• W2071897480 countsByYear W20718974802016 @default.
• W2071897480 countsByYear W20718974802017 @default.
• W2071897480 countsByYear W20718974802018 @default.
• W2071897480 countsByYear W20718974802019 @default.
• W2071897480 countsByYear W20718974802020 @default.
• W2071897480 countsByYear W20718974802021 @default.
• W2071897480 countsByYear W20718974802022 @default.
• W2071897480 countsByYear W20718974802023 @default.
• W2071897480 crossrefType "journal-article" @default.
• W2071897480 hasAuthorship W2071897480A5017989879 @default.
• W2071897480 hasAuthorship W2071897480A5041024027 @default.
• W2071897480 hasAuthorship W2071897480A5072803870 @default.
• W2071897480 hasAuthorship W2071897480A5077975447 @default.
• W2071897480 hasAuthorship W2071897480A5088903163 @default.
• W2071897480 hasConcept C12554922 @default.
• W2071897480 hasConcept C127413603 @default.
• W2071897480 hasConcept C145822097 @default.
• W2071897480 hasConcept C154280991 @default.
• W2071897480 hasConcept C178790620 @default.
• W2071897480 hasConcept C185592680 @default.
• W2071897480 hasConcept C2778270854 @default.
• W2071897480 hasConcept C2779660836 @default.
• W2071897480 hasConcept C2779870022 @default.
• W2071897480 hasConcept C2780365088 @default.
• W2071897480 hasConcept C2780881558 @default.
• W2071897480 hasConcept C2988574769 @default.
• W2071897480 hasConcept C2992163139 @default.
• W2071897480 hasConcept C515207424 @default.
• W2071897480 hasConcept C54355233 @default.
• W2071897480 hasConcept C55493867 @default.
• W2071897480 hasConcept C57581600 @default.
• W2071897480 hasConcept C86803240 @default.
• W2071897480 hasConcept C87717796 @default.
• W2071897480 hasConcept C95444343 @default.
• W2071897480 hasConceptScore W2071897480C12554922 @default.
• W2071897480 hasConceptScore W2071897480C127413603 @default.
• W2071897480 hasConceptScore W2071897480C145822097 @default.
• W2071897480 hasConceptScore W2071897480C154280991 @default.
• W2071897480 hasConceptScore W2071897480C178790620 @default.
• W2071897480 hasConceptScore W2071897480C185592680 @default.
• W2071897480 hasConceptScore W2071897480C2778270854 @default.
• W2071897480 hasConceptScore W2071897480C2779660836 @default.
• W2071897480 hasConceptScore W2071897480C2779870022 @default.
• W2071897480 hasConceptScore W2071897480C2780365088 @default.
• W2071897480 hasConceptScore W2071897480C2780881558 @default.
• W2071897480 hasConceptScore W2071897480C2988574769 @default.
• W2071897480 hasConceptScore W2071897480C2992163139 @default.
• W2071897480 hasConceptScore W2071897480C515207424 @default.
• W2071897480 hasConceptScore W2071897480C54355233 @default.
• W2071897480 hasConceptScore W2071897480C55493867 @default.
• W2071897480 hasConceptScore W2071897480C57581600 @default.
• W2071897480 hasConceptScore W2071897480C86803240 @default.
• W2071897480 hasConceptScore W2071897480C87717796 @default.
• W2071897480 hasConceptScore W2071897480C95444343 @default.
• W2071897480 hasIssue "3" @default.
• W2071897480 hasLocation W20718974801 @default.

• next