FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2071936366> ?p ?o ?g. }

• W2071936366 endingPage "218" @default.
• W2071936366 startingPage "207" @default.
• W2071936366 abstract "IncHI1 plasmids are one of the few plasmids known to mediate multiple antibiotic resistance in Salmonella typhi. These plasmids are temperature-sensitive for transfer and R27 is the prototype plasmid. DNA sequencing within the Tra2 region of R27, encoding genes involved in mating pair formation, identified trhC encoding the TrhC protein of 90,000 Da, which was visualized using an in vitro transcription/translation system. Expression of the TrhC protein also identified two smaller protein products of approximately 23 and 25 kDa which are predicted to be protease digestion products. The migration of these smaller products changed when the reactions were run at 28 vs 37 degrees C. The TrhC protein contained a putative nucleotide triphosphate-binding protein and exhibited sequence similarities with several other proteins implicated in bacterial conjugation, including TraC (F), TraB (pKM101), VirB4 (Ti), TrbE (RP4). Phylogenetic analysis showed TrhC was most closely related to TraC. Mini-Tn10 insertions in trhC generated transfer defective mutants, and no pili were specified by the trhC mutants. The trhC gene appeared to be a hot spot for transposon insertion as 37.5% mapped into this open reading frame. One trhC mutant, pDT2990, was able to be complemented by a cloned trhC gene giving a transfer frequency of 1 x 10(-3) transconjugants per recipient in an 18-h mating, whereas the wild-type transfer frequency of R27 was 1 x 10(-2) transconjugants per recipient." @default.
• W2071936366 created "2016-06-24" @default.
• W2071936366 creator A5005307958 @default.
• W2071936366 creator A5041987249 @default.
• W2071936366 creator A5067262682 @default.
• W2071936366 creator A5082599033 @default.
• W2071936366 creator A5088889403 @default.
• W2071936366 date "1999-05-01" @default.
• W2071936366 modified "2023-09-26" @default.
• W2071936366 title "Sequencing and Characterization of Salmonella typhi Plasmid R27 (Incompatibility Group HI1) trhC, a Transfer Gene Encoding a Potential Nucleoside Triphosphate-Binding Domain" @default.
• W2071936366 cites W124504601 @default.
• W2071936366 cites W1482870182 @default.
• W2071936366 cites W1516724217 @default.
• W2071936366 cites W1519108518 @default.
• W2071936366 cites W1563064375 @default.
• W2071936366 cites W1570512758 @default.
• W2071936366 cites W1586072175 @default.
• W2071936366 cites W1670015181 @default.
• W2071936366 cites W180568440 @default.
• W2071936366 cites W1841890078 @default.
• W2071936366 cites W1855475827 @default.
• W2071936366 cites W1866548929 @default.
• W2071936366 cites W1912451872 @default.
• W2071936366 cites W1931518744 @default.
• W2071936366 cites W1945265201 @default.
• W2071936366 cites W1973396871 @default.
• W2071936366 cites W1993999527 @default.
• W2071936366 cites W1994389014 @default.
• W2071936366 cites W2010642258 @default.
• W2071936366 cites W2019410656 @default.
• W2071936366 cites W2029591287 @default.
• W2071936366 cites W2029987769 @default.
• W2071936366 cites W2030793012 @default.
• W2071936366 cites W2035007261 @default.
• W2071936366 cites W2036765224 @default.
• W2071936366 cites W2040581338 @default.
• W2071936366 cites W2043680413 @default.
• W2071936366 cites W2044555340 @default.
• W2071936366 cites W2052438423 @default.
• W2071936366 cites W2055043387 @default.
• W2071936366 cites W2058688042 @default.
• W2071936366 cites W2063450941 @default.
• W2071936366 cites W2087196806 @default.
• W2071936366 cites W2090649262 @default.
• W2071936366 cites W2103937037 @default.
• W2071936366 cites W2104017821 @default.
• W2071936366 cites W2106882534 @default.
• W2071936366 cites W2111472993 @default.
• W2071936366 cites W2127114049 @default.
• W2071936366 cites W2137565578 @default.
• W2071936366 cites W2138270253 @default.
• W2071936366 doi "https://doi.org/10.1006/plas.1999.1394" @default.
• W2071936366 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10366526" @default.
• W2071936366 hasPublicationYear "1999" @default.
• W2071936366 type Work @default.
• W2071936366 sameAs 2071936366 @default.
• W2071936366 citedByCount "9" @default.
• W2071936366 countsByYear W20719363662016 @default.
• W2071936366 countsByYear W20719363662019 @default.
• W2071936366 crossrefType "journal-article" @default.
• W2071936366 hasAuthorship W2071936366A5005307958 @default.
• W2071936366 hasAuthorship W2071936366A5041987249 @default.
• W2071936366 hasAuthorship W2071936366A5067262682 @default.
• W2071936366 hasAuthorship W2071936366A5082599033 @default.
• W2071936366 hasAuthorship W2071936366A5088889403 @default.
• W2071936366 hasConcept C104317684 @default.
• W2071936366 hasConcept C143065580 @default.
• W2071936366 hasConcept C153911025 @default.
• W2071936366 hasConcept C167625842 @default.
• W2071936366 hasConcept C174832134 @default.
• W2071936366 hasConcept C22744801 @default.
• W2071936366 hasConcept C2994552716 @default.
• W2071936366 hasConcept C47289529 @default.
• W2071936366 hasConcept C4918238 @default.
• W2071936366 hasConcept C54355233 @default.
• W2071936366 hasConcept C547475151 @default.
• W2071936366 hasConcept C86803240 @default.
• W2071936366 hasConceptScore W2071936366C104317684 @default.
• W2071936366 hasConceptScore W2071936366C143065580 @default.
• W2071936366 hasConceptScore W2071936366C153911025 @default.
• W2071936366 hasConceptScore W2071936366C167625842 @default.
• W2071936366 hasConceptScore W2071936366C174832134 @default.
• W2071936366 hasConceptScore W2071936366C22744801 @default.
• W2071936366 hasConceptScore W2071936366C2994552716 @default.
• W2071936366 hasConceptScore W2071936366C47289529 @default.
• W2071936366 hasConceptScore W2071936366C4918238 @default.
• W2071936366 hasConceptScore W2071936366C54355233 @default.
• W2071936366 hasConceptScore W2071936366C547475151 @default.
• W2071936366 hasConceptScore W2071936366C86803240 @default.
• W2071936366 hasIssue "3" @default.
• W2071936366 hasLocation W20719363661 @default.
• W2071936366 hasLocation W20719363662 @default.
• W2071936366 hasOpenAccess W2071936366 @default.
• W2071936366 hasPrimaryLocation W20719363661 @default.
• W2071936366 hasRelatedWork W1571771606 @default.
• W2071936366 hasRelatedWork W1927614236 @default.
• W2071936366 hasRelatedWork W1933230878 @default.
• W2071936366 hasRelatedWork W1976052285 @default.

• next