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• W2071995145 abstract "The KdpD/KdpE two-component system of Escherichia coli regulates expression of the kdpFABC operon encoding the high affinity K+ transport system KdpFABC. The input domain of KdpD comprises a domain that belongs to the family of universal stress proteins (Usp). It has been previously demonstrated that UspC binds to this domain, resulting in KdpD/KdpE scaffolding under salt stress. However the mechanistic significance of this domain for signaling remains unclear. Here, we employed a domain swapping approach to replace the KdpD-Usp domain with four homologous domains or with the six soluble Usp proteins of E. coli.Full response to salt stress was only achieved with a chimera that contains UspC, probably due to unaffected scaffolding of the KdpD/KdpE signaling cascade by soluble UspC. Unexpectedly, chimeras containing either UspF or UspG not only prevented kdpFABC expression under salt stress but also under K+ limiting conditions, although these hybrid proteins exhibited kinase and phosphotransferase activities in vitro. These are the first KdpD derivatives that do not respond to K+ limitation due to alterations in the N-terminal domain. Analysis of the KdpD-Usp tertiary structure revealed that this domain has a net positively charged surface, while UspF and UspG are characterized by net negative surface charges.The Usp domain within KdpD not only functions as a binding surface for the scaffold UspC, but it is also important for KdpD signaling. We propose that KdpD sensing/signaling involves alterations of electrostatic interactions between the large N- and C-terminal cytoplasmic domains." @default.
• W2071995145 created "2016-06-24" @default.
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• W2071995145 date "2009-01-01" @default.
• W2071995145 modified "2023-10-07" @default.
• W2071995145 title "Domain swapping reveals that the N-terminal domain of the sensor kinase KdpD in Escherichia coli is important for signaling" @default.
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• W2071995145 doi "https://doi.org/10.1186/1471-2180-9-133" @default.
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