Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072016303> ?p ?o ?g. }
- W2072016303 endingPage "271" @default.
- W2072016303 startingPage "266" @default.
- W2072016303 abstract "The type VI secretion system (T6SS) is a machine evolved by Gram-negative bacteria to deliver toxin effectors into target bacterial or eukaryotic cells. The T6SS is functionally and structurally similar to the contractile tail of the Myoviridae family of bacteriophages and can be viewed as a syringe anchored to the bacterial membrane by a transenvelope complex. The membrane complex is composed of three proteins: the TssM and TssL inner membrane components and the TssJ outer membrane lipoprotein. The TssM protein is central as it interacts with both TssL and TssJ, therefore linking the membranes. Using controlled trypsinolysis, a 32.4 kDa C-terminal fragment of enteroaggregative Escherichia coli TssM (TssM 32Ct ) was purified. A nanobody obtained from llama immunization, nb25, exhibited subnanomolar affinity for TssM 32Ct . Crystals of the TssM 32Ct –nb25 complex were obtained and diffracted to 1.9 Å resolution. The crystals belonged to space group P 6 4 , with unit-cell parameters a = b = 95.23, c = 172.95 Å. Molecular replacement with a model nanobody indicated the presence of a dimer of TssM 32Ct –nb25 in the asymmetric unit." @default.
- W2072016303 created "2016-06-24" @default.
- W2072016303 creator A5012234034 @default.
- W2072016303 creator A5013451849 @default.
- W2072016303 creator A5018688681 @default.
- W2072016303 creator A5018842385 @default.
- W2072016303 creator A5053471257 @default.
- W2072016303 creator A5062852451 @default.
- W2072016303 creator A5076532911 @default.
- W2072016303 creator A5085766942 @default.
- W2072016303 date "2015-02-19" @default.
- W2072016303 modified "2023-10-18" @default.
- W2072016303 title "Production, crystallization and X-ray diffraction analysis of a complex between a fragment of the TssM T6SS protein and a camelid nanobody" @default.
- W2072016303 cites W1537441966 @default.
- W2072016303 cites W1965670038 @default.
- W2072016303 cites W1974170290 @default.
- W2072016303 cites W1976555793 @default.
- W2072016303 cites W1995590346 @default.
- W2072016303 cites W1996433261 @default.
- W2072016303 cites W1998292458 @default.
- W2072016303 cites W2009335881 @default.
- W2072016303 cites W2012109285 @default.
- W2072016303 cites W2021643610 @default.
- W2072016303 cites W2023657507 @default.
- W2072016303 cites W2043186783 @default.
- W2072016303 cites W2053493806 @default.
- W2072016303 cites W2068911499 @default.
- W2072016303 cites W2076683915 @default.
- W2072016303 cites W2081991152 @default.
- W2072016303 cites W2090535305 @default.
- W2072016303 cites W2095553811 @default.
- W2072016303 cites W2108921801 @default.
- W2072016303 cites W2110770898 @default.
- W2072016303 cites W2122095711 @default.
- W2072016303 cites W2133394828 @default.
- W2072016303 cites W2144081223 @default.
- W2072016303 cites W2145859310 @default.
- W2072016303 cites W2146080841 @default.
- W2072016303 cites W2152627116 @default.
- W2072016303 cites W2154845344 @default.
- W2072016303 cites W2169610187 @default.
- W2072016303 cites W4248872320 @default.
- W2072016303 doi "https://doi.org/10.1107/s2053230x15000709" @default.
- W2072016303 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4356300" @default.
- W2072016303 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25760699" @default.
- W2072016303 hasPublicationYear "2015" @default.
- W2072016303 type Work @default.
- W2072016303 sameAs 2072016303 @default.
- W2072016303 citedByCount "6" @default.
- W2072016303 countsByYear W20720163032015 @default.
- W2072016303 countsByYear W20720163032017 @default.
- W2072016303 countsByYear W20720163032023 @default.
- W2072016303 crossrefType "journal-article" @default.
- W2072016303 hasAuthorship W2072016303A5012234034 @default.
- W2072016303 hasAuthorship W2072016303A5013451849 @default.
- W2072016303 hasAuthorship W2072016303A5018688681 @default.
- W2072016303 hasAuthorship W2072016303A5018842385 @default.
- W2072016303 hasAuthorship W2072016303A5053471257 @default.
- W2072016303 hasAuthorship W2072016303A5062852451 @default.
- W2072016303 hasAuthorship W2072016303A5076532911 @default.
- W2072016303 hasAuthorship W2072016303A5085766942 @default.
- W2072016303 hasBestOaLocation W20720163035 @default.
- W2072016303 hasConcept C104317684 @default.
- W2072016303 hasConcept C12554922 @default.
- W2072016303 hasConcept C146587185 @default.
- W2072016303 hasConcept C178790620 @default.
- W2072016303 hasConcept C185592680 @default.
- W2072016303 hasConcept C203036418 @default.
- W2072016303 hasConcept C2778827730 @default.
- W2072016303 hasConcept C41625074 @default.
- W2072016303 hasConcept C547475151 @default.
- W2072016303 hasConcept C55493867 @default.
- W2072016303 hasConcept C60987743 @default.
- W2072016303 hasConcept C86803240 @default.
- W2072016303 hasConceptScore W2072016303C104317684 @default.
- W2072016303 hasConceptScore W2072016303C12554922 @default.
- W2072016303 hasConceptScore W2072016303C146587185 @default.
- W2072016303 hasConceptScore W2072016303C178790620 @default.
- W2072016303 hasConceptScore W2072016303C185592680 @default.
- W2072016303 hasConceptScore W2072016303C203036418 @default.
- W2072016303 hasConceptScore W2072016303C2778827730 @default.
- W2072016303 hasConceptScore W2072016303C41625074 @default.
- W2072016303 hasConceptScore W2072016303C547475151 @default.
- W2072016303 hasConceptScore W2072016303C55493867 @default.
- W2072016303 hasConceptScore W2072016303C60987743 @default.
- W2072016303 hasConceptScore W2072016303C86803240 @default.
- W2072016303 hasIssue "3" @default.
- W2072016303 hasLocation W20720163031 @default.
- W2072016303 hasLocation W20720163032 @default.
- W2072016303 hasLocation W20720163033 @default.
- W2072016303 hasLocation W20720163034 @default.
- W2072016303 hasLocation W20720163035 @default.
- W2072016303 hasLocation W20720163036 @default.
- W2072016303 hasLocation W20720163037 @default.
- W2072016303 hasOpenAccess W2072016303 @default.
- W2072016303 hasPrimaryLocation W20720163031 @default.
- W2072016303 hasRelatedWork W1521888210 @default.
- W2072016303 hasRelatedWork W1896531654 @default.