Matches in SemOpenAlex for { <https://semopenalex.org/work/W2072048351> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2072048351 endingPage "115" @default.
- W2072048351 startingPage "111" @default.
- W2072048351 abstract "Prior experience with cultures of cerebrospinal fluid lymphocytes indicated a need to develop methods for culturing small numbers of cells. Peripheral blood lymphocytes (PBL) were obtained from 20 normal volunteers. Standard microcultures using 100 X 10(3) PBL/0.2 ml and cultures with 50, 25 and 12.5 X 10(3) in 0.1 ml or 0.2 ml were established in RPMI 1640 with autologous plasma. These cultures were incubated with PHA (1--30 microgram) for 3, 4 and 5 days, pulsed with [3H]thymidine and harvested. In unstimulated cultures, cpm declined linearly with decreasing cell numbers. Standard cultures (100 X 10(3) PBL/0.2 ml) had maximal PHA stimulation (80,916 +/- 6394) at day 3 with 30 microgram PHA. Other 0.2 ml cultures had lower cpm. By culturing 25 X 10(3) PBL in 0.1 ml for 3 days cpm were 82,874 +/- 6875 with 30 microgram PHA and 77,153 +/- 6022 with 15 microgram PHA and were similar to standard cultures. Similar cpm were seen with 12.5 X 10(3) PBL in 0.1 ml after 4 days with 30 micrograms of PHA (80,838 +/- 6674) and with 15 micrograms of PHA (72,860 +/- 6243), and also after 5 days with 30 micrograms of PHA (86,703 +/- 6732) and with 15 micrograms of PHA (74,066 +/- 6388). The maximal response (126,578 +/- 6580) was seen with 25 X 10(3) PBL/0.1 ml at day 4 with 30 micrograms of PHA. By decreasing culture volume to 0.1 ml and increasing time, the number of cells necessary to give PHA responses similar to standard cultures can be reduced by 75--88%." @default.
- W2072048351 created "2016-06-24" @default.
- W2072048351 creator A5073396044 @default.
- W2072048351 date "1979-08-01" @default.
- W2072048351 modified "2023-10-16" @default.
- W2072048351 title "Lymphocyte cultures with small numbers of cells" @default.
- W2072048351 cites W1539061345 @default.
- W2072048351 cites W18589478 @default.
- W2072048351 cites W1974148619 @default.
- W2072048351 cites W1992890491 @default.
- W2072048351 cites W2024657291 @default.
- W2072048351 cites W2059638832 @default.
- W2072048351 cites W2069177404 @default.
- W2072048351 cites W2081902426 @default.
- W2072048351 cites W2088964195 @default.
- W2072048351 cites W2338826776 @default.
- W2072048351 doi "https://doi.org/10.1016/0022-1759(79)90060-7" @default.
- W2072048351 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/383846" @default.
- W2072048351 hasPublicationYear "1979" @default.
- W2072048351 type Work @default.
- W2072048351 sameAs 2072048351 @default.
- W2072048351 citedByCount "0" @default.
- W2072048351 crossrefType "journal-article" @default.
- W2072048351 hasAuthorship W2072048351A5073396044 @default.
- W2072048351 hasConcept C126322002 @default.
- W2072048351 hasConcept C153911025 @default.
- W2072048351 hasConcept C16685009 @default.
- W2072048351 hasConcept C185592680 @default.
- W2072048351 hasConcept C202751555 @default.
- W2072048351 hasConcept C24998067 @default.
- W2072048351 hasConcept C2777108182 @default.
- W2072048351 hasConcept C2777761686 @default.
- W2072048351 hasConcept C43617362 @default.
- W2072048351 hasConcept C51296783 @default.
- W2072048351 hasConcept C54355233 @default.
- W2072048351 hasConcept C55493867 @default.
- W2072048351 hasConcept C71924100 @default.
- W2072048351 hasConcept C81885089 @default.
- W2072048351 hasConcept C86803240 @default.
- W2072048351 hasConceptScore W2072048351C126322002 @default.
- W2072048351 hasConceptScore W2072048351C153911025 @default.
- W2072048351 hasConceptScore W2072048351C16685009 @default.
- W2072048351 hasConceptScore W2072048351C185592680 @default.
- W2072048351 hasConceptScore W2072048351C202751555 @default.
- W2072048351 hasConceptScore W2072048351C24998067 @default.
- W2072048351 hasConceptScore W2072048351C2777108182 @default.
- W2072048351 hasConceptScore W2072048351C2777761686 @default.
- W2072048351 hasConceptScore W2072048351C43617362 @default.
- W2072048351 hasConceptScore W2072048351C51296783 @default.
- W2072048351 hasConceptScore W2072048351C54355233 @default.
- W2072048351 hasConceptScore W2072048351C55493867 @default.
- W2072048351 hasConceptScore W2072048351C71924100 @default.
- W2072048351 hasConceptScore W2072048351C81885089 @default.
- W2072048351 hasConceptScore W2072048351C86803240 @default.
- W2072048351 hasIssue "2" @default.
- W2072048351 hasLocation W20720483511 @default.
- W2072048351 hasLocation W20720483512 @default.
- W2072048351 hasOpenAccess W2072048351 @default.
- W2072048351 hasPrimaryLocation W20720483511 @default.
- W2072048351 hasRelatedWork W1508014848 @default.
- W2072048351 hasRelatedWork W1579921607 @default.
- W2072048351 hasRelatedWork W1997681068 @default.
- W2072048351 hasRelatedWork W2021206498 @default.
- W2072048351 hasRelatedWork W2034290576 @default.
- W2072048351 hasRelatedWork W2059655161 @default.
- W2072048351 hasRelatedWork W207131278 @default.
- W2072048351 hasRelatedWork W2077466322 @default.
- W2072048351 hasRelatedWork W2415525173 @default.
- W2072048351 hasRelatedWork W4313310028 @default.
- W2072048351 hasVolume "29" @default.
- W2072048351 isParatext "false" @default.
- W2072048351 isRetracted "false" @default.
- W2072048351 magId "2072048351" @default.
- W2072048351 workType "article" @default.